砄bjective: To study the rule of the degradation of HA artificial bone(HAB). Methods: The samples of HAB were immersed in PBS or distilled water (DW),the changes of the shape, weight, compressive strength of the samples and pH value of the immersion solutions were measred at the intervals of 2 or 4 weeks until 28 weeks. Results: At 4 weeks, HAB began to be degraded, 8 weeks later, the speed of the degradation slowed down. From 4 to 12 weeks, the compressive strength decreased rapidly. The pH value of the immersion solutions decreased from 2 to 12 weeks,but increased from 12 to 28 weeks when it was close to the neutral value. Conclusion: HA artificial bone can be degraded in PBS solution, and the degradation can cause noticeable changes of the compressive strength of the material and pH value of the immersion solution.

0.05),respectively. Conclusion: The results indicate that the multidrug resistance of MEC-1/5Fu may be related to the higher expression of P-gp170 protein.

Objective:To study the effects of 17 beta-estradiol (E2) on the proliferation and metastasis potential of salivary gland mucoepidermoid carcinoma M_3SP_4 cells. Methods:The effects of E2 on the potential of proliferation and metastasis of M_3SP_4 cells were investigated by cell counting, MTT assay, clonegenetic assay, in vivo tumour growth and metastasis assay in nude mice.The expression of VEGF,c-erbB-2,Ki-67 and P16 in M_3SP_4 cells was examined by immunohistochemistry assay. Results:E2 increased the proliferation of M_3SP_4 cells at 10~-10-10~-5 mol/L. E2 at 10~-7 mol/L showed the strongest effects.After treatment with E2 at 10~-7 mol/L,the population doubling time of M_3SP_4 cells decreased by 10.8%,clonegenecity increased by 225%, VEGF,c-erbB-2 and Ki-67 expression increased,P16 decreased.In the in vivo assay,the tumour growth increased by 65% and metastasis increased by 609% in nude mice treated with E2 at 0.005 2 mg/d. Conclusion:E2 may stimulate the proliferation and metastasis potential of M_3SP_4 cells.

0.05), on the 4th day 7.56?6.87 and 10.00?7.07 (P0.05). After therapy all the data of electrocardiogram, blood routine examination and blood biochemical test of the cases were without clinical significance. Conclusion:50 g/L amlexanox is effective and safe in the treatment of RAU.

Objective:To investigate the effects of 17-? estradiol (E2) on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells.Methods:The effects of E2 on the proliferation of SAcc83 cells were studied by MTT assay and flow cytometry.The invasion potential of the cells was investigated with chicken embryo heart invasion assay.The in vivo growth and metastasis of SAcc83 cell induced tumor were studied in ovary-resected female nude mice. Results:E2 at 10 -7 ,10 -8 and 10 -9 mol/L promoted the proliferation of SAcc83 cells. E2 at 10 -7 mol/L showed the strongest growth stimulation effects and increased the cell proliferation by 34.4%.With exposure time of 1,2 and 3 d it increased the proliferation index by 33.3%,40.9% and 17.8% respectively;with the exposure time of 12,18 and 24 h,it increased S phase cells in cell cycle by 12.3%,3.3% and 9.7% respectively. Neoplasm developed in all nude mice after subcutaneous transplantation of 106 cells.In the mice treated with 0.2 ml of E2 at 9.55 ?10 -5 mol/L every day for 15 days neoplasm volume doubling time was 61.5 h,that in control mice 76.5 h.In lung metastasis trial, 42 days after tail injection of the cells the metastasis foci in E2 treated mice inreased by 41.2%.Conclusion:E2 may increase the proliferation and metastasis of adenoid cystic carcinoma cells.

Objective:To establish a culture method of human salivary gland epithelial cells and to study their growth characteristics in vitro.Methods:Tissue explant technic was employed to culture human salivary gland epithelial cells in serum free medium (SFM),1∶1 DMEM/F12 and 1∶1 DMEM/F12 containing 25 ml/L fetal boven serum (FBS) respectively.The morphology of the cultured cells was observed by phase contract microscope.The cell growth was studied by cell counting.The cells were identified by HE staining,PAS staining and SP staining.Results:Growth of human salivary gland epithelial cells was observed in primary culture in the three kinds of medium in all 10 cases. The cultured cells were epidermoid, positive for cytokeratin, negative for Vimentin and positive for PAS staining. The cells in SFM could be subcultured for five passages,while only for one passage in the other two kinds of medium.Conclusion:SFM is superior to serum free 1∶1 DMEM/F12 or 1∶1 DMEM/F12 containing 25 ml/L FBS for the culture of human salivary gland epithelial cells.

7;there were 32 b ands in tumor group. ③SDS PAGE showed that the number of protein bands with relative molecular mass of 77 000,50 000-52 000,38 000-30 000 increased in the tumour group. Conclusion: In the saliva of periodontitis indivauals there are more basic proteins,the relative molecular mass of the prot ein in the saliva of patients with tumor is different from that of health contro ls.

Objeact:To evaluate the effects of the exogenous PTEN gene on morphology of the highly metastatic mucoepidermoid carcinoma cell line M3SP2. Methods: Morphological observation of vehicle transfected M3SP2-pBp cells and PTEN transfected M3SP2-PTEN cells was performed with inverted microscope, HE staining and optical microscope, scanning electronic microscope and transmisson electronic microscope. Results: Compared with the control cells of M3SP2-pBp, the exogenous PTEN expressing cells M3SP2-PTEN showed morphological changes, such as vacuole denaturalization, shrinkage, less microvillus, chondriosome swelling, lysosome amalgamation, and chromatin agglutination. Conclusion: The exogenous PTEN gene may induce denaturalization, necrosis, and apoptosis of the highly metastatic mucoepidermoid carcinoma cells.

Objective:To evaluate the effect of gtfB specific antisense phosphorothioate-modified oligodeoxyribonucleotides(PS-ODNs) on S.mutans sucrose-dependent adherence.Methods:Antisense oligodeoxyribonucleotide targeted to gtfB sequence 709~726 bp(PS-ODN1) and 3 479~3 497 bp(PS-ODN2) were synthesized.Natural genetic transformation of S.mutans with PS-ODN1 and PS-ODN2 was respectively performed.Adhesion of S.mutans to saliva coated hydroxyapatite was examined by crystal violet staining and destain with absolute ethanol.The absorbance at 620 nm was measured by plate reader(the absorbance value derived from the wells without sucrose was used as background and was subtracted).Results:The adhesion ability of the strains treated with antisense PS-ODN was significantly lower than that of the control(P

0.05).Conclusion:Secnidazole is effective and safe in the treatment of pericoronitis.