Objective To analyze the hereditary background of common species from Rhodiola L. in Yunnan Province. Methods Fifty-nine samples of six populations in three natural medicinal species from Rhodiola L. were studied by RAPD method. Results The percentage of polymorphic bands (PPB) of three populations from R. crenulata were 37.97%, 46.15%, and 39.45% respectively; the Nei's gene diversities (h) were 0.110 3, 0.143 7, and 0.137 1, respectively; the Shannon's information indes (I) were 0.173 6, 0.222 1, and 0.206 7, respectively; at the special level, the PPB was 70.47%, I was (0.258 3); the genetic differentiation (G_(st)) was 0.263 0. The PPB of two populations from R. fastigiata were 43.67% and 44.42%, I were 0.215 3 and 0.217 4, respectively; at the special level, the PPB was 65.51%, G_(st) and I were 0.313 2 and 0.313 9, respectively; the PPB of R. yunnanensis was 36.97%, I was 0.188 1. Conclusion RAPD molecular marker could be used to molecular authentication and genetic diversity analysis of medicinal species from Rhodiola L.

Objective To investigate the application of heat-clearing and detoxifying Liangxue prescription decocting-free granules in puerperal infection and its clinical curative effect.Methods 97 cases patients with puerperal infection were chosen in the hospital from Dec.2012 to Dec.2014, according to the digital method, which were randomly divided into experimental group (n=49) and control group (n=48).The two groups were given metronidazole injection 250 mL/d, and 0.2 g mesylate levofloxacin added into 5% glucose injection 250 mL, intravenous drip, twice per day.On the basis of above therapy, experimental group were treated with heat-clearing and detoxifying Liangxue prescription decocting-free granules, orally, twice per day, one bag each time.Both of two groups were treated for 2 weeks as one course.Clinical symptom score, hemodynamic indexes, inflammatory factor, blood biochemical indexes and efficacy were observed and compared between two groups.Results Total effective rate of experimental group was 95.92%, which was significantly higher than 75.00% of control group ( U =3.58, P<0.01 ) .After treatment, the cell count of white blood cell ( WBC) and neutrophilic granulocyte ( GRAN ) , values of middle cell percentage (%MON ) , neutrophilic granulocyte ratio (%GRAN ) , and lymphocyte percentage (%LYM) in both of two groups significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the serum IL-6 and TNF-αlevels in two groups decreased compared with before treatment ( P<0.05 ) , and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the hemodynamic indexes including cell aggregation index, whole blood viscosity ( high, medium and low shear ) and plasma viscosity significantly improved compared with before treatment than control group ( P<0.05 ) , and the above indexes in experimental group significantly improved compared with control group (P<0.05).After treatment, the score of hypogastralgia, chills and fever, uterine body tender and a funicular tenderness of attachment area, lochia score and total score significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group significantly were lower than control group ( P<0.05 ) .Conclusion The heat-clearing and detoxifying Liangxue prescription decocting-free granules can significantly improve the inflammatory symptoms in treatment of puerperal infection, and its mechanism of action may improve microcirculation, reduce inflammatory reaction.The clinical efficacy of therapy is obviously better than western medicine treatment alone, which can be popularized in clinical application.

No abstract available.
Humans ; Hypogonadism* ; Male*

No abstract available.
Growth Hormone* ; Receptors, Somatotropin*

Objective To investigate the characteristic changes of auditory brainstem response (ABR) of nor‐mal hearing infants .Methods A total of 212 cases (327 ears) normal hearing infants were enrolled for ABR test . According to age ,167 ears was in 1~3 months age group ,105 ears was in 3~6 months age group ,55 ears was in 6~12 months age group .Each group consisted of ABR date were statistically analyzed .Results For 75 dB nHL click sound stimulus ,the peak latency and interpeak latency of Ⅲ ,Ⅴ ,Ⅰ — Ⅲ ,Ⅲ — Ⅴ ,Ⅰ — Ⅴwaves of normal hearing infants progressively shortened as they grew older except the wave Ⅰ .The ABR thresholds were in significantly difference among the groups .The amplitude ratio of wave V/I increased with the grewing age .Each group of data showed statistically significant differences .When the stimulus intensity reached at thresholds ,the occurrence rate of wave I ,III and V were different as they grew older .Conclusion Infants with auditory nervous system was in con‐stant development and improvement ,all parts of the auditory pathway of neural development was not balanced .

[ABSTRACT]OBJECTIVEThe influnence of observation on the antisense cytokeratin 13 (CK13) gene in nasopharyngeal carcinoma HNE1 cell transplantation tumor radiation sensitivity.METHODSHNE1 cell lines can be divided into control group: the control group (HNE1 cell) and lentivirus (transfection slow virus empty carrier) group and experimental group: HNE1-anti-CK13a (transfection antisense CK13a slow virus) and HNE1-anti-CK13b (transfection antisense slow virus CK13b) four groups, set up a corresponding animal model, after radiotherapy by flow cytometry, immunohistochemistry, PCR, Tunel method and Westernblotting detection.RESULTSThe cell cyclede tection of plasmid transfection slow virus group compared with control group after radiotherapy G2/M phase of the block were significantly prolonged; Immunohistochemical results showed emigration tumor CK13 expression decreased in the experimental group; Tunel method to detect apoptosis necrosis rate found that the experimental group,apoptosis rate significantly decreased; Western blotting detection caspase3 apoptosis markers. PCR to detect CDC25mRNA level decreased obviously.CONCLUSIONAntisense CK13 gene by regulating the cell cycle and apoptosis can reduce HNE1 transplantation tumor radiotherapy of nasopharyngeal carcinoma (NPC) sensitivity, and with the caspase 3 apoptotic pathways and CDC25 signaling pathways.

The family of HSP60 belongs to heat shock proteins with highly species conservatism and some important biologic functions. It can help other proteins for their assembling, folding and translocating, and plays a role in protecting cells against injuries and other types of stress. In addition, HSP60 is frequently recognized by the immune system as predominant antigens during infections and the progression of certain autoimmune diseases and might provide a novel strategy for the development of immunotherapeutics. This review focuses on distribution, molecular chaperone mechanism, function and gene expression regulation of HSP60. [

Objective To assess the ability of the new software SonoAVC to measure follicular volume and to compare these volume calculations with those made by conventional methods. Methods Three-dimensional ultrasound imaging was used to acquire volumetric data from the ovaries of 50 women undergoing controlled ovarian stimulation as part of in-vitro fertilization treatment. SonoAVC was used to automatically measure the volume of follicles and virtual organ computer-aided analysis(VOCAL) was also applied to measure volume, which was used as the standard of volume measurement. And the three perpendicular diameters (xyz diameters) of follicles were manually measured by conventional two-dimensional(2D) ultrasound, which were used to estimate volume using the sphere formula. And the validity of each technique was compared using limits of agreement. Results One hundred and seventy-six follicles were studied. SonoAVC and VOCAL provided highly accurate and repeatable follicular volume measurements in all cases. Volumes estimated from manually derived follicular diameter measurements were less accurate and repeatable. VOCAL took more time in follicular volume of measurement than SonoAVC and conventional 2D displays. Conclusions SonoAVC provides highly accurate, repeatable,and efficient measurements of follicular volume.

The ras-mitogen activeted protein kinase pathway is responsible for regulation of many cel-lular processes, including differentiation, cytoskeletal organization, and protein trafficking. Much attention has been paid to them and their role in cancer therapy recently, and its inhibitor pathways are applied to clinical tes-ting, such as, Raf kinse inhibitors, MEK inhibitor, Src kinase inhibitor.

Objective To analyze the diagnostic value of CT and MRI in spinal trauma.Methods 41 cases of spinal trauma in patients with spinal CT scan and MRI image data were retrospectively analyzed and compared.Results MRI examination revealed 41 cases of spinal fractures,CT showed 38 cases of fracture; MRI examination revealed 10 cases of bone contusion,CT scan found 1 case of bone contusion;MRI examination revealed 15 cases of anterior longitudinal ligament injury,12 cases occurred posterior longitudinal ligament injury,CT scan found that the anterior longitudinal ligament and posterior longitudinal ligament injury of 2 cases of MRI detection rate was significantly higher than CT(P ＜ 0.05).120 bone chips CT findings,MRI examination revealed 83 cases of bone chips,the difference was statistically significant(P ＜ 0.05).Conclusion CT and MRI of spinal trauma are two commonlyways of imaging examination.There are advantages and disadvantages of CT examination of the bone chips shift and so can be clearly shown MRI for ligament injury.Bone contusion can be clearly demonstrated for the general compression fractures in MRI examination.Commimcted fracture should be added to do a CT scan.