The theory of planned behavior is used to study physician's behavior of clinical pathway compliance,on which the model of decision making mechanism for such compliance was built.This aims at exploring the relationship between the behavioral risk factors,behavioral belief and behavioral intention, and the occurrence,and recommend on further regulation of physicians'behavior and policies in hospital management.

By searching relevant data from the PubMed database, Chinese National Knowledge Infrastructure (CNKI) database and Wanfang database, a comprehensive analysis and review regarding acupuncture for cerebral ischemia reperfusion injury (CIRI) in recent 10 years were performed. The results showed that acupuncture could inhibit the inflammatory reaction, reduce oxidative stress injury, restrain brain edema formation, inhibit apoptosis, promote neural and vascular regeneration, etc. Acupuncture methods used included electroacupuncture, scalp acupuncture, eye acupuncture and "consciousness-restoring resuscitation needling", etc. The existing problem was that the intervention action of acupuncture was mainly focused on inhibiting inflammatory reaction and oxidative stress injury, and the study on apoptosis and neural and vascular regeneration was needed. It is suggested that from the aspect of multiple target points, the intervention mechanism of acupuncture for CIRI should be systemically studied in the future, which could provide new idea for clinical diagnosis and treatment on ischemic cerebrovascular diseases.
Acupuncture Therapy ; history ; Animals ; Apoptosis ; Brain Ischemia ; history ; surgery ; History, 21st Century ; Humans ; Oxidative Stress ; PubMed ; Reperfusion Injury ; etiology ; history ; physiopathology ; therapy

Objective: To study the effect of down-regulation of eukaryotic translation initiation factor 4E (EIF 4E ) induced by the siRNA expression plasmid on the proliferation and cell cycle of human breast cancer cell line MDA-MB-231. Methods: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed and then transfected into breast cancer MDA-MB-231 cells by LipofectAMINE2000. The expressions of EIF4E and cyclin D1 mRNAs and proteins were examined by RT-PCR, Western blotting and the immunocytochemistry method, respectively. The MTT method, plate colony formation assay and flow cytometry were performed to detect the proliferation ability, cell colony formation rate and cell cycle distribution of MDA-MB-231 cells transfected with siRNA expression plasmid. Results: The siRNA expression plasmid pGPU6/GFP/Neo-EIF4E was constructed successfully. The expressions of EIF4E and cyclin D1 mRNAs and proteins were all decreased markedly after transfection with pGPU6/GFP/Neo-EIF4E (P <0.05). The growth of MDA-MB-231 cells in the pGPU6/GFP/Neo-EIF4E-tranfected group was inhibited and the cell colony formation rate was statistically decreased (P<0.05) as compared with those in the blank control and the pGPU6/GFP-transfected groups. The result of flow cytometry showed that the percentage of cells in G1 phase in the pGPU6/GFP/Neo-EIF4E-tranfected group was higher than that in the blank control group (71.30%±0.47% vs 53.10%±0.43%); however, the percentage of cells in S phase was signifcantly lower (12.53%±0.13% vs 26.47%±0.38%, P <0.05). Conclusion: EIF 4E siRNA can downregulate EIF 4E gene expression in human breast cancer MDA-MB-231 cells, suppress cell proliferation to some extent, and increase the number of cells in G1 phase with a decreased number of cells in S phase. EIF 4E gene may become one of the important molecular targets to breast cancer. Copyright© 2011 by the Editorial Board of Tumor.

Objective To inverstigte the feasibility of video-assisted thoracoscopic surgery(VATS) be used in grass-roots hospital. Methods 46 patients were treated with video-assisted thoracoscopic surgery or video-assisted minithoracotomy surgery (VAMT) including bullectomy, lobectomy or wedge resection of lung, lung volume reduction surgery, esophageal tumor resection, clearance of hemathorax,partial pericardial,et al. Results There was no operative mortality. Complications occurred in 2 cases(4.3%). All patients were perfectly recovered by treatment. The overall follow-up was 6 months. Conclusion VATS is a safe,effective and new technique of thoracic surgery. More attentions must be payed to prevent and reduce complication in order to generalize the use of grass-roots hospital.

Objective To treat the sunken upper eyelids in the midd le and older patients with a modified procedure so as to improve esthetic appeara nce of the patients. Methods Autolipoparticles collected from patients' abdomen were injected into the sunken upper eyelids beneath the o rbital septum. 3ml of the lipoparticles were injected for each upper eyelid as u sual. The blepharoplasty was also performed for most of the patients with lower eyelids bag before or during the operation. Results The decrepit phenomena of eyes in all the patients were improved by the operations. Owing to the phenomena that the sunken upper eyelids were associated with the fa t bags of lower eyelid in most patients, it was inferable that the sunken upper eyelids were probably caused by the tissues ptosis in the orbit. Con clusions The sunken upper eyelids associated with fat bags of lower eyelids are the decrepit phenomena of eyes in middle age and old people. The sun ken upper eyelids can be corrected by the method of autolipoparticle injection. After the operation the appearance of patients' eyes is obviously improved. The method is easily performed and its curative effect is reliable.

Objective: To examine T-lymphocyte subsets of peripheral blood and splenic cells in rat with acute pancreatitis(AP),to investigate the changes of immune functional status in the development of acute pancreatitis.Methods: AP model was developed in adult male rats by retrograde injection of 4% sodium taurocholate in the pancreatic duct.T-lymphocyte subsets( CD3,CD4,CD8) of peripheral blood and splenic cells was measured by Flow Cytometer.Results: There was no singificant change in the stage of MAP.In the stage of SAP,the levels of CD3 and CD4 and CD4/CD8 of peripheral blood were decreased,the decrease of T-lymphocyte subsets in splenic cells was more obvious than those in peripheral blood.Conclusion:Spleen shows a negative immunologic function in the stage of SAP and promote the development of AP.

Total and water soluble selenium in soil, and total, tetravalent and he-xavalent selenium in drinking water were determined fluorimetrically by 2, 3-diaminonaphthalene. The digestive mixture was composed of sulphuric, perchloric acids and sodium molybdate 0.2 g of soil and 20 ml of water samples were sufficient for analysis when their selenium concentrations were at the level of 0.1 ppm and 1 ppb respectively. Coefficient of variation and recovery for soil was 12% and 92 ?6%, and for water was 4.3-5.8% and 98 ?2% respectively. Tetravalent and hexavalent selenium content could be measured differentially by the addition or omission of hydrochloric acid in ordinary water samples containing only small amounts of organic matter. In addition to tetravalent selenium substantial amounts of hexavalent selenium may occur occasionally in drinking water and it is thus necessary to add hydrochloric acid to the final digests to completely reduce hexavalent selenium to the tetravalent state for the estimation of total selenium in water samples.

Objective To observe the clinical efficacy of docetaxel and nedaplatin (NDP)in treatment of patients with epithelial ovarian cancer. Methods 50 cases with epithelial ovarian cancer collected in Shifang People’s Hospital from 2009 January to 2010 January were randomly divided into treatment group and control group,each had 25 cases.Treatment group were given docetaxel and nedaplatin,while control group were given carboplatin. The efficacy and adverse reactions in two groups were observed and compared.Results The recent efficacy in two groups were 76.00% and 52.00%, respectively,and the difference was significant (P <0.05 ).Compared with control group, the incidence of leucopenia was 36.00% and thrombocytopenia was 24.00% in treatment group,there was no significant difference between them.The incidences of nausea,vomiting and alopecia in treatment group were 12.00%,8.00% and 44.00%,which lower than that in control group (40%,28% and 64%),the differences between two groups were all significant(P<0.05 ).The two years survival rates in treatment group was 37.5%,and 28% in control group,there was no significant difference between two groups.Conclusion Docetaxel and nedaplatin has good clinical efficacy in treatment of epithelial ovarian cancer.

Biological ultrasonic (US),photoacoustic (PA) and magnetoacoustic (MA) imaging techniques had similar imaging principles and complementary characteristics.Combined US,PA or MA imaging can provide joint images of biological tissues which present complementary information for more comprehensive characterization of tissues in contrast to single imaging technique.The pathological tissues could be precisely located and their morphology and composition could be accurately identified.This review presented current progresses in combined PA-US,MA-US and US-PA-MA imaging techniques,espeically endoscopic imaging,focusing on the technical aspects of integration and their applications in biomedicine.

OBJECTIVE:To observe therapeutic efficacy and safety of nedaplatin combined with paclitaxel in the treatment of advanced cervical cancer. METHODS:Totally 100 patients with advanced cervical cancer were randomly divided into observation group(50 cases)and control group(50 cases). Both groups were given 6MV linear accelerator radiotherapy combined with intra-cavitary irradiation. Based on it,control group was additionally given Cisplatin injection 20 mg/m2,d1+Paclitaxel injection 35 mg/m2,d1 intravenously within 3 h. Observation group was additionally given Nedaplatin for injection 20 mg/m2,d1+Paclitaxel injection intravenously(same usage and dosage as control group). A treatment course lasted for a week,and both groups received 6 courses of treatment. Short-term efficacies of 2 groups were observed,and the levels of vascular endothelial growth factor A(VEGF-A), VEGF-C and VEGF-D,lymphatic microvessel density(LVD),microvessel density(MVD),toxic reaction were also observed be-fore and after treatment. RESULTS: Total response rate(52.00% vs. 32.00%)and disease control rate(86.00% vs. 66.00%)of ob-servation group were significantly higher than those of control group,with statistical significance(P<0.05). After treatment,the levels of VEGF-A,VEGF-C and VEGF-D,LVD,MVD in 2 groups were significantly lower than before treatment,and the obser-vation group was significantly lower than the control group,with statistical significance(P<0.05). The incidence of thrombocyto-penia in observation group was significantly higher than control group,and the incidence of nausea and vomiting was significantly lower than control group,with statistical significance(P<0.05). CONCLUSIONS: Nedaplatin combined with paclitaxel can im-prove short-term efficacy of patients with advanced cervical cancer,reduce gastrointestinal reaction,VEGF level and inhibit the generation of tumor vessel,but great importance should be attached to platelet toxic reaction.