Objective To explore the diagnostic value of carcino embryonic antigen (CEA),squamous cell carcinoma antigen(SCC),human papilloma virus-E7 (HPV-E7) in cervical carcinoma.Methods A total of 107 cases of women patients treated in hospital from July 2013 to July 2015 accorded to the pathological examination results were divided into cervical cancer group 60 cases and CIN group 47 cases,another 50 cases of healthy people were selected as control group, and serum expression levels of HPV-E7, CEA and SCC in the three groups were detected by enzyme-linked immunosorbent assay.Results The serum HPV-E7, CEA and SCC in cervical cancer group were significantly higher than those in CIN group and control group (P<0.05).There was no significant difference in serum HPV-E7, CEA and SCC between CIN group and control group.The levels of serum HPV-E7, CEA and SCC in stage I-II were significantly lower than those of stage III-IV in patients with cervical cancer, and the difference with statistically significant between two groups(P<0.05).The area under the ROC of HPV-E7 was significantly higher than that of CEA and SCC (Z=2.914,2.951, P<0.05), and there was no significant difference in the area under the ROC between CEA and SCC (Z=1.580,P=0.057).Conclusion The serum HPV-E7, CEA and SCC in cervical cancer patients are significantly higher, the diagnosis value of HPV-E7 is higher which is expected to become one of the effective indicators of cervical cancer diagnosis.

Objective To investigate characteristics of emotional intelligence(EI)and the ways of occupational stress coping in nurses as well as the predictive validity of EI on coping strategies.Methods 464 nurses from general hospitals were collected and evaluated with psychometric instruments including:Wong's Emotional Intelligence Scale(WEIS)and Nurse Ways of Coping Questionnaire.Data were conducted by Logistic regress analysis according to key themes.Results (1)Nurses' demographic characteristics had effects to the ways of occupational stress coping and emotional intelligence.However,there was no statistical difference of El varied with education levels(P＞0.05).(2)Both problems solving and positive recognition are main coping styles of nurses towards occupational stress.Either avoidance or self-reproach was seldom coping strategies of nurses toward occupational pressures(2.60±0.55;2.60±0.75;1.37±0.69;respectively).(3)El was correlated with coping styles(r=-0.123～0.253,P＜0.05,P＜0.01).Conclusion Four dimensions of EI positively or negatively predicted six kinds of occupational stress coping strategies separately.

Objective To study the prevalence of chronic kidney disease(CKD) and risk factors among adults received examina-tion in Yongchuan district of Chongqing .Methods 10 280 residents(older than 18 years) were selected ,who had health examina-tion in the Yongchuan Affiliated Hospital ,Chongqing Medical University ,Yongchuan Hospital of Traditional Chinese Medicine and Yongchuan people's hospital from January 2012 to December 2013 .Patients with CKD were identified by the examination dates .All of them were conducted with a questionnaire survey ,physical examination ,and renal damage and related risk indicator factors moni-toring for more than 3 months .Results Eligible dates of 10 021 subjects were enrolled in the study .The prevalence of albuminuria was 5 .1% ,hematuria in 8 .3% ,reduced renal function in 1 .4% .The CKD population prevalence rate was 15 .7% ,and the recogni-tion was 6 .4% .The Logistic regression model showed that hyperuricemia ,nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age contributed to the development of CKD .Conclusion The prevalence of chronic kidney disease is 15 .7% and the recognition is 6 .4% in urban healthy examination population of Yongchuan .Independent risk factors associated with CKD are hyperuricemia , nephrolithiasis ,diabetes mellitus ,hypertension ,obesity and age .

Thrombolytic therapy is a safe and effective way to cure thrombosis. Developing the safe,effective and cheap therapeutic thromolytic agents are important to prevent and cure thrombosis. In recent years,several fibrinolytic enzymes have been found in the resources of Asian traditional fermented foods,such as Japanese natto,Korea Chungkook-Jang,Chinese Douchi and fermented shrimp paste. These fibrinolytic enzymes secreted by food-grade microorganisms are safe and effective,and are potent thrombolytic agents. The physical and biochemical properties,thrombolytic characteristics,and the potential applications of these fibrinolytic enzymes from Asian fermented food,are reviewed.

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

OBJECTIVE: To explore the institutional pricing factors influencing the improvement of drug quality and stimulate the reasonable alignment of the drug quality and price. METHODS: clarify the interrelationship between the connotations of drug quality, drug quality and drug price with the assistance of the questionnaire formulated according to the findings of structured interviews with the experts based on the descriptive statistical analysis and structural equation modeling analysis of the 25 institutional factors influencing drug prices. RESULTS: The fact that drug price exerts impact on the improvement of drug quality is not a mere function of the drug pricing rules. Factors such as the business bribes in drug dealership, the large number of drug distribution links, the high fees and the ranking of pharmaceutical benefits scheme as well as the internal cost management of the enterprise are some of the key factors that affect the pricing policies. CONCLUSION: The price regulations and policies should be adjusted with clarifying the internal cost management policies as the focus. The pricing policies should be made to encourage the retraining initiatives, energy reservation and emissions reduction as well as the consistency between the drug quality and prices so as to resolve the issue of lacking the driving force to enhance the drug qualities in the manufacturing process.

Objective To investigate the effects of different processing methods on the appearance, quality components and processing efficiency of Lonicera macranthoides that produced in Guizhou Province, in order to provide basis for the optimization of processing methods. Methods Eleven processing methods were used such as the traditional method group (sun drying, sun drying after steaming, drying in shade, and drying in shade after steaming), constant temperature drying group (50, 60, 70, and 80 ℃) and variable temperature drying group (50→60 ℃, 50→70 ℃, 50→80 ℃). Then the indicators such as color, aroma, moisture, total ash, acid insoluble ash, chlorogenic acid, total saponins, processing time, and drying rate were measured. Results According to all the indicators, the comprehensive correlation degree of the 50 ℃ and the variable temperature drying method was between 0.94 and 0.96, which was much higher than other methods, and the comprehensive correlation degree of 50→60 ℃ degrees was the highest, reaching 0.959 8. Conclusion In conclusion variable temperature drying method is a better processing method for L. macranthoides. This method can be used as an important technical means for large-scale processing.

Objective: To design and prepare intensive training models for key steps of complicated laparoscopic urology surgery,and to use them for training of new learners. Methods: Based on the key steps for laparoscopic surgery in urology, such as radical prostatectomy, nephron-sparing surgery (NSS) and pyeloplasty for ureteropelvic junction obstruction (UPJO), we prepared the models of retropubic vesicourethral anastomosis, partial nephrectomy and pyeloplasty UPJO using intestines of pigs and chickens, kidney of pigs, swim bladder and ureter of pigs, etc. The models were used to train the new learners and the training outcomes were observed. Results: The models of retropubic vesicourethral anastomosis, partial nephrectomy and pyeloplasty UPJO were successfully prepared, and they satisfactorily simulated various procedures; they costed less, were easy to make, and had rich resources. The models were suitable for training of new learners. After intensive training with the models, the new learners could master the skills and became more self-confident. Conclusion: We have successfully prepared the models for intensive training of the key steps of complicated laparoscopic surgery in urology; the model can meet the demand for training of the new learners.

Objective To explore the risk factors of asthma in children with bronchiclitics.Methods One hundred and two cases hospita-lized patients with bronchiolitis were analyzed and followed-up for 2-7 years,items of follow-up included sex,morbidity age,food feeding,severity of disease,atopy physique,family history of atopy/asthma,environment exposure,passive smoking,and so on.The factors were carried on the Logistic regression analysis to determine the influence on later years to having recurrent asthma.Results Forty-seven cases had transferred asthma,which was remarkably higher than the domestic urban survey data(0.12%-3.34%).The multi-factors Logistic regression analysis demonstrated that environment(OR 6.099,95%CI 1.824～20.396),passive smoking(OR 49.222,95%CI 8.219-294.774),atopy physique (OR 45.548,95%CI 6.799-305.117)and family history of atopy/asthma(OR 47.921,95%CI 8.626-266.226)were the risk facter of asthma( Pa