One hundred fifteen surgical specimens were studied by gram-stain, aerobic and anaerobic cultu- res, as well as gas chromatography, respectively. One hundred seventy six strains, including ninety five aerobic and eighty one anaerobic strains, were isolated. The results suggested that diagnosis of anaerobic bacteria by gas chromatography could be accomplished within 1 h. The specificity of this method was 98.11% and the sensitivity, 90.47%. In addition, the early identification of anaerobic bacteria could be made also. There is higher value for rapid diagnosis of anaerobic infection with gas chromatography.

Objective: To explore correlation between serum uric acid (UA) level and heart failure severity in patients with chronic pulmonary heart disease (CPHD).Methods:According to New York Heart Association (NYHA) cardiac function classification, a total of 98 CPHD patients were divided into class I~II group (n=36), class III group (n=31) and class IV group (n=31).Another 30 healthy subjects were enrolled as healthy control group simultaneously.Serum UA level was measured in all groups.Real time three-dimensional color Doppler echocardiography was used to measure left ventricular ejection fraction (LVEF) and left ventricular end-diastolic dimension (LVEDd), and the results were compared among all groups.Results: Compared with healthy control group, there were significant rise in serum UA level[(342.4±101.7) μmol/L vs.(459.6±110.3) μmol/L]and incidence rate of hyperuricemia (33.33% vs.58.16%) in CPHD group, P<0.05 both;along with NYHA class increased, there were significant rise in serum UA level[(350.7±103.4) μmol/L vs.(422.3±106.7) μmol/L vs.(491.8±113.2) μmol/L], LVEDd [(59.2±10.1)mm vs.(64.3±9.8)mm vs.(68.9±10.6)mm], and significant reduction in LVEF[(37.7±9.8)% vs.(31.9±8.6)% vs.(28.4±8.1)%]in CPHD patients, P<0.05 all;linear correlation analysis indicated that serum UA level was significant positively correlated with LVEDd (r=0.67, P=0.026), and significant inversely correlated with LVEF (r=-0.86, P=0.033).Conclusion: Serum uric acid level can be used as a reliable index evaluating heart failure severity in patients with chronic pulmonary heart disease.

To study the clinical features and diagnosis of synovitis,acne,pustulosis,hyperostosis,osteitis syndrome(SAPHO) syndrome.One case of SAPHO syndrome was reported and the related data of SAPHO syndrome were reviewed.The main clinical features of the patient were articulatio carpi synovitis,acne,cervical rib hyperostosis,articulatio sternoclavicularis and osteitis,So the diagnosis of SAPHO syndrome was made.Though SAPHO syndrome is rare with yet unknown prevalence,it still can be seen in clinical practice,and can be diagnosed by careful examination.

Objective To explore the specific causes of common peroneal nerve injury, characteristics of such injured nerve's electrophysiological performance and its diagnostic value.Methods The clinical and neurophys-iological examination data of 7 patients with common peroneal nerve injury were retrospectively analyzed.Results The patients of common peroneal nerve injury cause for long time squatting down.Compared with the healthy side, potential amplitude of the deep peroneal nerve or the superficial proneal nerve of the injured side changes more obviously.On the average the amplitude fell by about 50% with the largest drop by 88%.EMC indicated that the muscle controlled by injured nerve has spontaneous potential,reduced recruitment and so on.Conclusion Long time squatting down was relatively rare cause for common peroneal nerve injury.Neural physiological detection could provide more specific positioning diagnosis and differential diagnosis for common peroneal nerve injury,had better clinical application value.

Objective To observe the therapeutic effect of eucalyptol limonene and pinene enteric soft capsules on elder community acguired pneumonin (CAP) patients.Methods Use the retrospective cohort study methods,233 patients,above 65 year old,were breught into the study,101 patients were in the eucalyptol limonene and pinene enteric soft capsules group and 132 patients were in the control group.The clinical symptoms and length of stay in hospital were recorded.Results The improvement of clinical symptoms on the fifth day of the eucalyptol limonene and pinene enteric soft capsules group was better than that of control group (U =2.37,P ＜ 0.05).And the length of stay in hospital of the eucalyptol limonene and pinene entetic soft capsules group was shorter(U =3.06,P ＜ 0.05).Conclusion Eucalyptol limonene and pinene enteric soft capsules can quickly improve the elder CAP patients clinical symptoms and shorten the length of stay in hospital.

Tumor Markers(Tumor Markers,TM) as a member of the tumor microenvironment components,change with the tumor microenvironment and the functional status of the body.Chinese medicine can improve the overall regulation of tumor micro-environment impact on the TM,which to some extent,the dynamic detection of TM can be reflected in the earlier short-term medical treatment of cancer patients.Based on TM,we clarified the relationship of differentiating disease and differentiating syndrome,type of syndrome and histology pathology type for tumor.This will provide new theoretical foundation for clinical anti-tumor studies from the microcosmic scope.

OBJECTIVE:To analyze the current status of counterfeit in China and indicate the difficultie of identification.METHODS:The main characteristics of counterfeit drugs were discussed by representative cases.RESULTS & CONCLUSION:It is suggested that the department of the drug administrave should establish a more scientific,reasonable standardization procedure of conterfeit drugs identification aiming directly at the characteristics of counterfeit drugs,which was more sufficient,intensive and practicable legal evidence for defining,fighting and preventing counterfeit drugs.

Neoadjuvant chemotherapy is one of the common means in multidisciplinary treatment of locally advanced breast cancer,with anthracyclines and taxanes as main chemotherapeutic drugs.In recent years,molecular target agents have significantly improved the efficacy of chemotherapy,as well as have provided more choices and ideas for clinical treatment.Currently,the widely studied molecular targeted drugs include trastuzumab,lapatinib,pertuzumab,T-DM1 and bevacizumab.

Exosomes are cell-derived vesicles that contain protein and RNA from the same source and function. The vesicle has a diam-eter between 30 and 100 nm. Exosomes are the natural carriers and have been recently used as drug delivery system for cancer treat-ment. MicroRNAs (miRNAs) function in RNA silencing and regulation of gene expression. They can exist as intracellular and extracellu-lar miRNAs. Extracellular miRNAs can function as secreted signaling molecules that affect receptor cell phenotype. They can also re-flect molecular changes in donor cells. Therefore, extracellular miRNAs can be potentially used for diagnosis and therapeutics. Studies show that the volume of exosomes in cancer patients' blood is higher than that in normal controls. The ability to package cancer-relat-ed miRNAs is a biological function of exosomes. In conclusion, specific miRNAs transferred by exosomes may play an important role in the pathogenesis of tumor or cancer. This paper presents a summary of research on exosomes as the carrier of miRNAs in the develop-ment and treatment of cancer.

Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.