1.Rapid determination of fetal Y-chromosome with polymerase chain reaction.
Sung Ho KANG ; Kyu Byung JUNG ; Ho Won HAN ; Young Chul KIM ; Sung Il NOH ; Ki Suk OH ; In Kwon HAN ; In Gul MOON
Korean Journal of Obstetrics and Gynecology 1993;36(3):321-325
No abstract available.
Polymerase Chain Reaction*
2.The Clinical Applicability of PCR and FISH in the Detection of Y-chromosome from Fetal Nucleated Red Blood Cells in Maternal Blood.
Jae Hyun CHUNG ; Kwan Ja JI ; Soon Ha YANG ; Jung Mi OH ; Cheong Rae ROH ; Young Kyu MOON ; Syng Wook KIM ; Je Ho LEE
Korean Journal of Obstetrics and Gynecology 1997;40(12):2692-2697
No abstract available.
Erythrocytes*
;
Polymerase Chain Reaction*
3.Detection of male-specific DNA by polymerase chain reaction.
Korean Journal of Perinatology 1993;4(3):391-400
No abstract available.
DNA*
;
Polymerase Chain Reaction*
4.Detection of HPV in cervical scrape specimens of cervical neoplasia using the polymerase chain reaction.
Seung Chul KIM ; Hak soon KIM ; Ju Cheol SONG ; Seo Ok KANG ; Young Bum CHA ; In Kwon HAN ; In Geol MOON ; Won Hee HAN ; Chong Taek PARK
Korean Journal of Obstetrics and Gynecology 1992;35(9):1269-1279
No abstract available.
Polymerase Chain Reaction*
5.Determination of sex by polymerase chain reaction (I).
Sang Hun CHA ; Tai Ho CHO ; Yong Sang SONG ; Hyo Pyo LEE
Korean Journal of Obstetrics and Gynecology 1991;34(11):1568-1573
No abstract available.
Polymerase Chain Reaction*
6.Detection of hepatitis viral nucleic acid sequences using polymerase chain reaction.
Korean Journal of Infectious Diseases 1991;23(4):229-233
No abstract available.
Hepatitis*
;
Polymerase Chain Reaction*
7.Sequencing of Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium and application for PCR to differentiate them
Journal of Preventive Medicine 2005;15(5):36-41
All Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium code for phase 1 of H antigen (H:1) (d, a, b, c and i antigen respectively). The genes were sequenced on Sanger's principle by automatic sequenser (ABI, 3100 Avant, Genetic Analyser). The primer pairs for the Salmonella species were designed on the basis of the collected sequences. The results showed that PCR with these primers can clearly differentiate the five Salmonella strains, especially between S. paratyphi B and S. typhimurium.
Polymerase Chain Reaction
;
Salmonella
8.Some application of PCR in microbiology
Journal of Practical Medicine 2002;435(11):25-27
In microbiology, PCR was applied very early and widely step by step to diagnose the etiology of the infection. Especially in case of the culture of microorganism was unsuccessfully implemented or is very difficult or patient used the antibiotic before admission because PCR can be implemented in the dead microorganism. PCR contributes to verify more correctly.
microbiology
;
Polymerase Chain Reaction
9.Detection of human papillomaviruses in cervical interepithelial neoplasia and invasive carcinoma by in situ polymerase chain reaction.
Joon Cheol PARK ; Tae Sang KIM ; Dong Ja KIM ; Han Ik BAE ; Jeong Ran KIM
Korean Journal of Obstetrics and Gynecology 2000;43(10):1738-1743
No abstract available.
Humans*
;
Polymerase Chain Reaction*
10.Rapid detection of Respiratory Pathogens using a Multiplex PCR assay among hospitalized children with Acute Respiratory Infection
Katherine B. Javier ; Josephine Anne Navoa-Ng ; Nikki Cotoco-Chu
Pediatric Infectious Disease Society of the Philippines Journal 2021;22(2):55-65
Background:
Acute respiratory infection (ARI) is a major cause of morbidity and mortality among children worldwide however, local data on the etiologic diagnosis of ARI are limited.
Objectives:
To determine the prevalence and the most commonly detected respiratory pathogens using a multiplex PCR assay, known as the Respiratory Panel, among hospitalized children with ARI and compare their clinical and laboratory differences.
Methods:
This is a cross sectional study of children with ARI who were tested with a multiplex PCR assay. Retrospective chart review was done on these patients admitted from January 2018 to February 2020.
Results:
There were 47 charts reviewed, mean age was 4.2 years old. Out of 47 patients, 36 (76.6%) tested positive for a pathogen. Respiratory syncytial virus (RSV) being the most common followed by Influenza A/H1-2009 and Human metapneumovirus (hMPV). Two patients had viral co-infections and no bacteria were detected on all subjects. 61.7% patients were started on antibiotics on admission. Fever and cough were the most common sign and symptom, respectively. Normal WBC (68% with neutrophilic predominance) and platelet were detected in 72.3% and 70.2% of patients, respectively; 50% of patients had normal CRP and 60.5% had abnormal findings on chest x-ray. Only the presence of chest x-ray findings was found to have a higher probability of yielding a positive Respiratory Panel p=0.27.
Conclusion
Among admitted patients with ARI, 76.6% tested positive for a respiratory pathogen. All were caused by viruses presenting as nonspecific manifestations – fever and cough. Clinical manifestations, CBC and CRP showed no association with the Respiratory Panel result while abnormal chest x-ray had a higher probability of yielding a positive Respiratory Panel result.
Multiplex Polymerase Chain Reaction
Result Analysis
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