Dioxin,as a kind of strong carcinogenic substance,a kind of environment hormone with tremendous harm,can get into human body through food chain and accumulate in fat of animals and persons,forming serious threat to human health. Dioxin has been included in the list of monitoring objects in food part of the global environment monitoring program as a new environmental pollutant by World Health Organization. In this paper,the toxicity,the source of dioxin and its impact on human health and prevention and control measures are introduced.

A briefly introduction of action mechanism of preservatives,deterioration caused by microorganism and the commonly used preservatives in cosmetics were presented in this paper. Sample pretreatment method and instrumental analysis method were summarized also. The popular preservatives in cosmetics were parabens. The consumption of new preservatives in cosmetics (iodopropynyl butylcarbamate,diazolidinyl urea,imidazolidinyl urea) is increasing. The analysis method of preservatives by the high-performance liquid chromatography was most widespread. Regarding kathon,the sensitivity of gas chromatography is higher than that of high-performance liquid chromatography. The other analysis methods are semi-quantitative thin layer chromatography and simultaneously determination water-soluble and the fat soluble preservatives capillary electrophoresis. The methods for determination of 55 limited preservatives of Hygienic Standard for Cosmetics(in 2002 version) are still not satisfactory,more methods should be developed.

The organophosphorus insecticides including a large group of insecticides were widely used in the world in the agriculture and household. Over 80% of the pesticides used in China are organophosphorus pesticides. Workers and the general population may be exposed to insecticides mixtures through water,air and the residue of food. After entering into human body,the insecticide mixtures can cause a combined toxicity. In this paper,the effects and toxicity mechanisms of combined exposures on health were expounded.

Recently,to assess the reproductive effects of bisphenol A (BPA),the environmental estrogen,has become a focus of stusy. Investigations,to date,have focused on the effects on the reproductive system including the morphogenesis of tissues and organs,endocrine and the toxic mechanisms of action,particularly in males. Based on the study results concerning BPA’s impacts on male reproduction,the further study directions in this field were presented in this paper.

The present situation of environment polluted by phthalate esters,the main kinds of phthalate esters in the air samples and water samples and the pretreatment and analysis technology of phthalate esters in different environment were reviewed in the present article. In the air samples ,di(2-ethylhexy) phthalate(DEHP),di-n-butyl phthalate(DBP) and dioctyl phthalate(DOP) were included,while diethyl phthalate(DEP),di(2-ethylhexy) phthalate(DEHP),dipropyl phthalate(DPrP),butyl benzyl phthalate(BBP),dioctyl phthalate(DOP) and dimethyl phthalate(DMP) could be mainly detected in the water samples.

Objective To develop an ion chromatogram method for determination of fluoride in the sea food and meat. Methods Under the acid condition,fluorine could be separated by distillation,then fluorine is absorbed by washing liquid. The electricity lead detection-ion chromatogram with Ionpac AS14 column was employed to determine the content of fluoride,3.5 mmol/L of sodium carbonate-1.0 mmol/L of soda as the washing liquid at 1.2 ml/ min. Results The linear range was 0.05-1.0 mg/L,the relative coefficient (r) was more than 0.999 7 (n=6),the lowest detection limit was 0.3 mg/kg,the relative standard deviation was 1.00%-3.85%,the recovery rates were 88.1%-99.0%. Conclusion This method presents a good effect of pretreatment and fluorine ion seperation and with a satisfactory recovery rate,precision and detection limit.

Objective To establish a method for rapid determination of the lead in air by water phase of solubilisation dithizone photometry. Methods The lead in air was determined by water phase solubilisation dithizone photometry with Tween20 as the solubilizer. Results The linear range was 1.0-100 mg/L(r≥0.999),RSDs were 1.36%～9.57%,the recovery rates were 97.4%-98.3%,the lowest detected concentration was 0.003 mg/m3. There was no significant difference between the result of this method and that of colorimetry. Conclusion The method was rapid,accurate and sensitive,this method is suitable for rapid determination of lead in the air.

Objective To establish a simultaneous determination method of cyclohexane,cyclohexanone and cyclohexanol in workplace air. Methods To collect the cyclohexane,cyclohexanone and cyclohexanol from the workplace air by pumping the air through the active carbon tube,resolve them with alcohol and then test the content of them by capillary gas chromatography. Results It was proved that these samples were separated effectively. The final measurement range of cyclohexane,cyclohexanone and cyclohexanol was 30-1 000 ?g/ml,20-1 000 ?g/ml and 20-880 ?g/ml respectively,the linear equation was y=257.4x+987.1 (r=0.999 9),y=239.9x+664.6 (r=0.9999 ) and y=192.4x+607.1 (r=0.999 9) respectively,the detection limit of was 1.5 ?g/ml,0.5 ?g/ml and 1.0 ?g/ml respectively. The minimum detectable concentration was 1.0 mg/m3,0.3 mg/m3 and 0.7 mg/m3 respectively (collected 1.5 L air sample). The efficiency of resolution was 90.1%,91.2% and 93.1% respectively. Conclusion The method is suitable for the simultaneous determination of the cyclohexane,cyclohexanone and cyclohexanol in the workplace air.

Objective To study the efficiency of the compound active carbon filter in reducing the content of free radicals in the smoke of cigarettes. Methods 9 trademarks of cigarettes installed the compound active carbon filters were collected and ESR was used to analyze the content of free radicals in the particle and gas phases. Results The results showed that the efficiency of the compound active carbon filters to reduce the content of free radicals in the cigarette smoke was not identical,some samples even showed an inverse result. Conclusion Based on the results of the present paper,it is not considered that the compound active carbon filter can always reduce the free radicals in the smoke of cigarettes.

Objective To study the effect of the low free radical cigarettes (test cigarettes) on the blood biochemical parameters,lung and polychromatophilic red cell micronucleus in rats. Methods 36 SD rats were randomly divided into the blank control group,the comparison smoke group and the experimental smoke group (low free radical cigarettes),12 in each. Let the rats smoke passively for 30 day,daily 12 cigarettes,then the determination of blood biochemical parameters and the polychromatophilic red cell micronucleus rate in the bone marrow,the pathological examination of the trachea and the lung tissues were conducted. Results Compared with the blank control,no significant changes were seen in the blood biochemical parameters and the polychromatophilic red cell micronucleus rate. The pathological examination of the lung tissues indicated that the pathological changes in the experimental smoke group were slight compared with the comparison smoke group. Conclusion No seriously adverse effects of low free radical cigarettes on the lung tissues of rats have been seen in the present study,that indicates that maybe the low free radical cigarette has an advantage in the health safety.