Objective To compare the content of saponin in Bark of Silktree Albizziae and Bark of Silktree Albizziae kaldora derived from different collection periods.Method Spectrophotometry was used to determine the contents of saponin.Result The contents of saponin in one-year Bark of Silktree Albizziae is different from two-year Bark of Silktree Albizziae(P0.05).The contents of saponin in multi-year Bark of Silktree Albizziae is different from multi-year Bark of Silktree Albizziae kaldora(P

Objective:To identify differential proteins expression of seminal plasma of varicocele patients and normal person,providing evidence for further exploring the molecular mechanism of infertility caused by varicocele.Methods:The differential proteins expression of seminal plasma of varicocele patients and normal person were characterized by means of two-dimensional gel electrophoresis(2-DE).Results:The well-resolved,reproducible 2-DE seminal plasma were obtained.The 38 differential protein spots were identified.Conclusion:The male infertility might due to the expression and modification of some proteins in seminal plasma of varicocele patient.Further analysis on the different proteins'expression helps revealing molecule mechanism of varicocele causing male infertility.

Objective:To study the influence of rt-PA and urokinase on the expression of matrix metalloproteinase(MMP-9) and hemorrhagic transformation after thrombolysis therapy in rats.Methods:A focal cerebral ischemia model of rat was established through embolization of left middle cerebral artery with autologous blood clots.rt-PA and urokinase were intravenously administrated 6 hours after ischemia at a dose of 10 mg/kg and 4 000 ?/kg.At the 24th hour after ischemia,all rats were killed.The expression of MMP-9 was detected with immunohistochemical staining and cerebral hemorrhage was quantified by spectrophotometer assay.Results:The expression of MMP-9 was increased after ischemia,and MMP-9 was significantly further upregulated after rt-PA and urokinase had been intravenously administrated.The average optical density(AOD) of MMP-9 in the sham operated group was 0.086?0.008,and increased to 0.158?0.010 after cerebral ischemia for 24 hours and then further increased to 0.225?0.034 and 0.228?0.022 in the rt-PA and urokinase thrombolytic therapy groups.Transformation and quantified intracerebral hemorrhage were significantly higher in the therapy groups as compared with the ischemic group.However,there was no difference between rt-PA group and urokinase group.Conclusion:rt-PA and urokinase treatment both increase the levels of MMP-9 after ischemia.MMP-9 might be involved in the hemorrhagic transformation after thrombolytic therapy with rt-PA and urokinase.

Objective:Evaluate the relationship of complications and indwelling time of double-J ureteral stents after ureterscopic holmium-laser lithotripsy. Methods:170 patients were divided into two groups randomly. groupⅠ(stents indwelling 4 weeks),groupⅡ(stents indwelling 2 weeks).Complications of two groups were compared. Results:During the indwelling time,two groups presented urinary irritative sypmtoms were 81(93%)and 51(61%)P

AIM: To observe the adverse effect and clinical efficiency of Y-shape plate internal fixation for treating comminuted distal humeral fracture. METHODS: A retrospective analysis was conducted in 18 patients with the comminuted distal humeral fracture,who were admitted in the First Affiliated Hospital of Nanyang Medical College from January 2003 to December 2006. All patients were treated with Y-shape stainless steel or Ti-alloy plate in 3-7 holes,then the response of materials and host was observed. The curative efficiency was assayed with Cassebaum score,and the healing of fractures was detected with X-ray film. During follow-ups,the pain and joint motion were observed. RESULTS: The mean operational time was 134 minutes,the mean value of intraoperative blood loss was 172 mL,and all the incisions were healed well. All the patients were followed up for 6 months,and the mean time for the fracture healing was 14 weeks. One case appeared delayed healing in the resection lesion,and Kirschner's wire was loosened in 2 cases. There was no infection,local inflammatory reaction or rejection reaction occurred. Among 18 patients,11 got excellent results,4 good,2 fair,1 poor,and the rate of excellent and good efficiency was 83.3%. CONCLUSION: Y-shape plate after implantation shows good biocompatibility with the human body,with the manifestations of good reduction of comminuted distal humeral fracture,firm internal fixation,and satisfactory functional recovery of elbow joint.

Objective: Metrological verification for the medical emergency vehicle terminal 3G system, in order to facilitate the accurate positioning, route correctly, timely treatment, high success rate, and increase the credibility and effectiveness of treatment process records provided in the verification process or perform the burden of adducing evidences for emergency first-aid course. Methods:Metrological verification the positioning error, azimuth error and velocity error of emergency medical 3G system terminal. Results:The vehicle terminal measurement device and measurement method, which can satisfy the requirements of verification terminal technology and provide a valid certificate. Conclusion:Metrological verification has the very good function to emergency medical vehicles to successfully treat patients and subsequent verification and perform the burden of adducing evidences.

Objective To compare the content of polysaccharide in Herba Scutellariae Barbatae derived from different places. Method Anthrone-sulphuric acid colrimetry was used. Result The contents of polysaccharide in Herba Scutellariae Barbatae derived from Mojiang Yunnan, Nantong Jiangsu, Huaning Hunan, Yongding Fujian, Lixin Anhui, Jiaoling Guangdong, Nanchang Jiangxi, Longsheng Guangxi, Honghu Hubei and Qingyuan Zhejiang were 8.12%, 7.61%, 6.61%, 6.07%, 5.84%, 4.61%, 3.15%, 2.69%, 2.65% and 2.34%, respectively. Conclusion The content of polysaccharide in Herba Scutellariae Barbatae from different places is quite different, and the highest is the one from Mojiang Yunnan.

Acute myeloid leukemia is a disease of malignant clone of the hematopoietic stem cell.The abnormity of molecular biology plays an important role in the pathogenesis of acute myeloid leukemia.More and more molecular abnormalities provide the basis of prognostic evaluation and target therapy.

Hematopoietic stem cell transplantation (HSCT) has become the frontline therapy for patients with multiple myeloma (MM), and for young patients with primary MM, early autologous stem cell transplantation (ASCT) is a standard treatment. In the era of novel drugs, the application of new pharmaceutics in the consolidation and maintenance therapy before and after HSCT can improve the efficacy of HSCT. The novel agents pose a challenge to the status of HSCT in relapsed and refractory myeloma. This article summarizes the recent clinical studies of HSCT in the era of novel agents to clarify the role of HSCT in treatment of MM.

Objective The purpose of this study is to investigate CD 46 expression in advanced invasive breast cancer and in normal breast tissues , and to analyze the correlation between CD 4 expression and effect of neoadjuvant chemotherapy ,exploring its clinical significance for the prognostic value of neoadjuvant chemotherapy in breast cancer .Methods Immunohistochemistry was employed to detect CD 46 expression of tissue samples from 60 patients with invasive breast cancer and 30 normal breasts .We further analyzed the relationship between effect of neoadjuvant chemotherapy and CD 46 expression ,and the prognostic significance for the survival .Rseults CD46-positive rate in breast cancer was apparently higher than that in normal breast tissues (81.67% vs. 46.67%,P<0.05).After neoadjuvant chemotherapy,the CD46 expression was significantly decreased (P <0 .05).There was a negative correlation between CD 46 expression and efficacy of chemotherapy ( P<0.05). CD46 expression in breast cancer could be affected in cancer invasion ,lymph node metastasis(P<0.05).Con-clusion CD46 expression in breast cancer patients is higher than that in healthy people ,and was negatively cor-related with efficacy of adjuvant chemotherapy .The increasing rate of CD 46 positive expression has predictive val-ue for the poor prognosis of patients .