Contrast-enhanced ultrasound(CEUS)can afford more information of the vascularization of pancreatic tumors.Adenocarcinomas are often hypovascularized as compared with surrounding tissue,while neuroendocrine tumors are hypervascularized lesions.The most common enhancement pattern of masses associated with pancreatitis ale isovascularizatiom CEUS can improve the diagnosis and differential diagnosis ability in pancreatic tumors.The clinical application value of CEUS is significant.

Objective To study the effects of γ-aminobutyric acid ( GABA) and sertraline on the CABAA(α) receptor and GABAB receptor of hippocampus neuron in rats of depression induced by acute stress. Methods The cognitive function of male SD (Sprague-Dawley) rats was screened through Y-maze. Then rats were randomly divided into five groups. Except control group,rats were injected intraperitoneally with double-distilled, GABA,sertraline or GABA + sertraline respectively. They were exposed to the forced-swimming stress test,which was to make acute stress model of depression. The space memory function in the maze was measured. Then the number of GABA-like immunoreactive neurons in hippocampus CA1 ,CA3 ,DG areas of rats were investigated with immunohistochemist. Results Compared with the model group,the immobility time in the forced-swimming test and the latency in the maze could be reduced significantly after pretreatment with GABA and sertraline (P < 0.01). The positive cell populations of CA1 ,CA3 region and DG gate region in the model group were significantly reduced compared with the control group (P<0.01). GABA could only improve the positive cell populations of GABAB receptor in CA3 region (P<0.01). In the sertraline group,the positive cell populations in hippocampus were increased obviously more than it in model group(P<0.01). The positive cell populations of hippocampus in the GABA + sertraline group in CA1 ( (82.83±8.72),(78.08±5.67)),in CA3((92.83±9.35),(76.00±3.97)),in the gate of DG( (35.00 ±1.41) ,(33.33±4.36)) increased significantly to the other groups (P< 0.05 or 0.01). Conclusion Ectogenic GABA could improve the sertraline' s effects of antagonizing acute depression effectively and the space memory function,and its mechanism may be involved in strengthening the expression of GABAA(α) receptor and GABAB receptor in hippocampus.

Objective To investigate the application of ureteral catheter in complicated gynecological laparoscopic surgery for the prevention of ureteral injury . Methods A total of 182 cases of gynecological laparoscopic surgery performed from January 2009 to November 2011 were selected as study group .Bilateral ureteral catheters were inserted under cystoscopy , and then laparoscopic surgery was performed under the instruction of the catheter .The catheter was immediately removed after the surgery .A total of 200 cases of laparoscopic surgery were selected as control group and preoperative ureteral catheterization was not applied in this group .The incidence rate of ureteral injury was compared between the two groups . Results The success rate of bilateral ureteral catheterization in the study group was 98.9%(180/182)and no ureteral injury occurred .In the control group, ureteral injury was found in 2 cases, which were converted to open surgery for ureter repair and double J stent was retained .The 2 cases recovered 2 months after the surgery.Bladder injury was found in 2 cases during the surgery , which received immediate repair assisted by surgeons from the department of urinary surgery and recovered 2 months after postoperative catheterization . No statistical difference was found in complication rate between the two groups ( P=0.125 ) . Conclusion The application of ureteral catheterization in complicated gynecological laparoscopic surgery can reduce the risk of intraoperative ureteral injury .

With acupuncture depth as a perspective, some related issues are discussed in this paper. Firstly, the possible reasons of divergence on needling depth are explained. Secondly, by literature retrieval, the advantages and disadvantages of the clinical efficacy of deep needling and shallow needling are explored, and the essential value of shallow needling is emphasized in promoting acupuncture. Thirdly, due to the placebo acupuncture that led to the controversy of the curative effect of acupuncture, it is crucial to establish a method of modern system of scientific research which should be suitable for the development of acupuncture. Finally, the "cun" in acupuncture is inappropriate to transform into millimeter, and the standardization of acupuncture should focus on the improvement of curative efficacy. From the acupuncture depth, it is clear that there are plenty of problems in acupuncture, which could not only limit the development of acupuncture, but also affect the future of acupuncture.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Needles

Adult ; Humans ; Laryngeal Neoplasms ; Male ; Neurofibroma ; Vocal Cords ; pathology

Chromatography, Ion Exchange ; Hemoglobins, Abnormal ; analysis ; Humans ; Male

Child ; Fibroma ; Humans ; Male ; Nasal Cavity ; pathology ; Nose Neoplasms

Objective: To establish an HPLC-MS/MS method for the determination of hypericin in rat plasma. Methods: The sample was analyzed by HPLC-MS/MS after the addition of internal standard and then protein precipitation using acetonitrile. The sepa-ration was carried out on an Ultimate C18 column (150 mm × 2. 1 mm,5. 0 μm). The mobile phase was composed of acetonitrile∶ 5 mmol·L-1 ammoniumacetate (containing 0. 1% formic acid) (90∶10) at a flow rate of 0. 5 ml·min-1 under 35℃. The detection was performed with multiple teactions monitoring ( MRM) using an electrospray ionization ( ESI) . The precursor/product ion transitions were monitored at m/z 503. 2→m/z 405. 1 for hypericin and m/z 355. 0→m/z 41. 9 for the internal standard pioglitazone ( anion mode). Results:The good linearity of hypericin was obtained within the range of 0. 1-13. 2 ng·ml-1. The correlation coefficient was more than 0. 99 and the lower limit of quantification was 0. 1 ng·ml-1. The extraction recovery was within the range of 84. 19%-98. 71%. The precision of intra-and inter-day was below 18. 47%. Conclusion: The method is fast, sensitive and accurate, which provides research basis for the clinical further study of hypericin.

Objective To study the effects of γ-aminobutyric acid (GABA) and sertraline on the cognition functions in acute stressed rats.Methods Y-type maze were used to test the cognitive function of Male SD (Sprague-Dawley) rats.They were exposed to the forced-swimming stress test,which was to make acute stress model of depression.Except control group,rats were pretreated with different doses of GABA and/or sertraline,then the time of immobility in the forced-swimming test and latency in the maze were observed individual.Results Compared with the model group[(1404.33±129.46)s] ,GABA could decrease the immobility time obviously in the forced-swimming test [(432.33±187.64) s ＞ (332.50±217.23) s,P ＜ 0.01].Otherwise the immobility time was prolonged with 0.50 g/kg GABA + 10 mg/kg sertraline (1086.17±411.80) s (P ＜ 0.05).Compared with the control group(105.00±32.18)s,the latency in the maze was prolonged remarkably in the model group (179.17±8.54) s(P＜ 0.05).Pretreatment with 10 mg/kg sertraline(100.30±21.01) s or GABA and sertraline groups (69.83±16.89) s,(86.83±13.11) s (P ＜ 0.05 or 0.01) could decrease the latency.Conclusion GABA could play its role of antagonizing acute depression.Meanwhile,pretreatment with appropriate doses of GABA and sertraline not ordy could decrease doses of two drugs but also improve the cognitive function in the acute stress rats.

Objective To observe the application effect of the Iodophors hygro-gauze on tracheotomy wound dressing. Methods Fifty patients who underwent tracheotomy with Ⅲ incision, were chosen to be divided into the control group and the observation group with 25 in each group. With saline water cleaning incision and surrounding skin, then disinfection with 0.5% iodophors sanitizers tampon. The observation group applied 0.5% Iodophors hygro-gauze on the wound, the control group used vaseline oil yarn. 24 hours after sample inspection, again samples were sent for bacteria analysis, bacterial colonies number, pathogenic bacteria growth and local situation of wound were observed and analyzed. Results The first results upon disinfection showed that bacterial colonies number, pathogenic bacteria growth between the two groups had no difference. After 24 hours sampling showed that the colony count and pathogenic bacteria growth of the observation group was obviously less than the control group. Local wound infection of the observation group was lighter than the control group. Conclusions 0.5% Iodophors hydro-gauze applied for tracheotomy wound dressing can effectively kill various pathogenic microorganism, prevent secondary infection, and also can prevent and treat wound infection.