Objective To investigate the characteristics and clinical application value of anti-double stranded DNA antibody de-tected by Crithidia indirect immunofluorescence assay method and enzyme linked immunosorbent assay method.Methods Eighty-five patients with systemic lupus erythematosus,20 disease controls and 75 healthy controls were selected.The serum anti-double stranded DNA antibody was detected simultaneously by the methods of Crithidia indirect immunofluorescence assay and enzyme linked immunosorbent assay and their diagnostic efficacies for detection were compared.Results For each method the positive rate in the systemic lupus erythematosus group was significantly higher than that in the disease control group and healthy control group. The difference had statistical significance (P <0.05).The positive rates of Crithidia indirect immunofluorescence assay and enzyme linked immunosorbent assay in the systemic lupus erythematosus group were 72.94% and 88.24% respectively,and the positive predictive value of enzyme linked immunosorbent assay is lower(P <0.05).Meanwhile the anti-double stranded DNA antibody con-centrations detected by enzyme linked immunosorbent assay method showed statistically significant difference among the active sys-temic lupus erythematosus group,the stable systemic lupus erythematosus group and the control group (P <0.05 )and presented linear trend.Conclusion Using Crithidia indirect immunofluorescence assay method to detect anti-double stranded DNA antibody for the systemic lupus erythematosus group has high specificity and is helpful for the diagnosis of systemic lupus erythematosus. Enzyme linked immunosorbent assay can be used to detect anti-double stranded DNA antibody concentration quantitatively,which is linearly related with systemic lupus erythematosus activity and the method is of high sensitivity,which can effectively screen the pa-tients with systemic lupus erythematosus.

Hilar cholangiocarcinoma is the most common types of cholangiocarcinoma,it is difficult to perform operation owing to its anatomic characterstics.Currently,radical resection is the most important prognostic factors of hilar cholangiocarcinoma.In addition,lymph node metastasis and biomarkers such as CA19-9,hepatocyte growth factor receptor,interleukin-8,and so on,are also closely related to the prognosis of hilar cholangiocarcinoma.This paper will review the relevant researches of China and abroad to summarize important prognostic factors of hilar cholangiocarcinoma above-mentioned.

Education, Nursing, Continuing ; General Surgery ; education ; Humans ; Physicians

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

Objective To evaluate the nutrition intervention on advanced colorectal cancer chemotherapy patients quality of life. Methods 70 colorectal cancer patients (NRS 2002 score was more than or equal to 3 points) with chemotherapy from January 1st, 2013 to June 31th, 2014 were collected. The patients were randomly divided into intervention group (35 patients) and the control group (35 patients). The intervention group received personalized recipe which was developed by a team containing nurse specialist, chief psychiatrist, family members and patients themselves. The control group was treated with a normal diet guidance. The two groups of patients when they were treated after the second days of the first admission (before intervention), the end of the 6th courses of chemotherapy and the 12th courses of chemotherapy were evaluated by the European Organization For Research and Treatment Quality of Life Questionnaire-C30 (EORTC QLQ-C30). Nutritional indicators and the total life condition of the two groups at different time were compared. Results There were 5 cases off in the intervention group and the control group respectively. After the 12th courses of chemotherapy, the intervention group serum albumin (ALB) and the mean serum prealbumin (PAB) were improved 4.10 and 74.14; and the control group were-0.09 and-0.08, there were significant differences between the two groups (t=3.202, 3.623, P<0.05).The total quality of life of the intervention group was 25.87 points while the control group was 35.13 points, there was significant difference between the two groups (t=3.321, P<0.05). The intervention group got the total health status was (72.28 ± 20.97) points after the intervention, and before the intervention was (56.67 ± 14.08) points. Meanwhile the total health status was statistically significant (t=7.01, P<0.05). Conclusions There is a significant nutritional improvement in colorectal cancer patients with chemotherapy after nutritional therapy leading by nurse specialist, which is worthy of application.

[Objective]To investigate the effect of injectable xuebijing on macrophage ofimervertebral disc hernial tissue.[Method]The pigs were used as animal models after surgery in the intervertebral disc.Macrophage in herniated disc tissue were observed with the immunohistochemical method after it was treated by injectable xuebijing.[Result]Macrophage were found in 11 of 16 herniated disc tissue in the model group,were found in 7 of 16 herniated disc tissue in the low dose xuebijing group,and were found in 6 of 16 herniated disc tissue in the high dose xuebijing group,compared to the model group,all P

Objective: To observe the effects of Thermafil obturat ion technique.Methods: 60 premolars with pulp pathema or periapical dese ase were divided into two groups with 30 in each at random,The teeth were treate d and the root canals were obturated by Thermafil obturation technique and later al condensation technique respectively. Preoperative and postoperative radiograp hs of each tooth were taken. Obturation time, incidence of pain during obturatio n and quality of obturation were compared.Results: Overfi lling was found in 11 cases in Thermafil group,while 3 in lateral condensation group( P 0.05).The average obturation tim e per canal of Thermafil and lateral condensation technique was 36.6 s and 247 .4 s respectuively ( P

[Objective]To evaluate the clinical treatment results for diastematomyelia treated by operation. [Method]Data from patients with diastematomyelia admitted to our hospital from May 1978 to January 2006 were reviewed. According to the classification, there were single-tube in 25 and double-tube in 96 patients. One hundred and two patients with diastematomyelia were treated by operation and ninteen by conservative treatment. The clinical results were retrospectively analyzed according to scoring neural function and monitoring the peak P40 of posterior trivial nerve cortical somatosensory evoked potential (PTNCSEP) before and after treatment.[Result]The total operative effective rate of double-tube diastematomyelia amounted to 86% during a follow-up period of 6 months to 15 years,more significant in patients with pain.The scores of neural function and PTNCSEP were significantly improved. But patients with single-tube diastematomyelia had no obvious change after operation.[Conclusion]The spinal cord is damaged severely in double-tube diastematomyelia and gradually aggravated.Operation can get symptomatic relief in many patients and terminate spinal cord lesion. So operation should be performed as soon as the diagnosis is made. The spinal cord is damaged slightly in single-tube diastematomyelia and surgery is not indicated.

0.05).Compared with control group,contents of sialic acid in the high and moderate dose groups,epididymal coefficient and contents of carnitine in the high dose group reduced significantly(P

Objective To seek an effective method for the treatment of grade ⅢB tibial fractures.Method From Oct. 1985 to Oct.1999, 26 cases of grade ⅢB tibial fractures were treated by early soft tissue coverage(including 12 cases of free muscle flaps and 14 cases of local muscle flaps), early fracture fixation(including 21 cases of external fixation and 5 cases of non-reamed intramedullary nail) and early bone grafting(15 cases).Results The follow up duration were from 10 to 32 months with an average of 15.5 months. The success rates of free and local muscle flaps were respectively 91.7 %(11/12) and 92.9 %(13/14)?Infection occurred in 5 cases (3 cases of local superficial infection and 2 cases of osteomyelitis). The time for bone union were from 17 to 66 weeks with an average of 42 weeks. Conclusion "San-Zao"therapy,especially early soft tissue coverage and early fracture fixation is on effective management for grade ⅢB tibial fractures with the advantages of promoting wound healing and bone union and decreasing the possibility of infection.