Acupuncture Therapy ; instrumentation ; methods ; Adult ; Aged ; Female ; Hot Temperature ; Humans ; Male ; Middle Aged ; Needles ; Spondylosis ; therapy ; Treatment Outcome ; Young Adult

Anti-Infective Agents ; administration & dosage ; Clinical Competence ; Hematologic Diseases ; Hospitals, Special ; manpower ; Humans ; Medical Staff, Hospital

Objective To investigate the efficacy of conservative treatment with laparoscopy for patients with ectopic pregnancy.Methods From January 2000 to December 2005,76 patients with ectopic pregnancy received conservative treatment by laparoscopy in our hospital.The data of the patients were analyzed retrospectively.Results In this series,fenestration of the Fallopian tube was performed on 42 cases,salpingostomy on 21,fimbrial expression on 8,and segmental resection and end-to-end anastomosis on 5.Salpingography showed that the Fallopian tube was patent after the operation in all of the patients.The mean operation time was(58.5?19.4) minutes(ranged from 35 to 105).The serum level of ?-hCG decreased to a normal range in 3 weeks postoperation.Follow-up was available in 61 cases for 1 year,during which 35 of the cases(57.4%,35/61) had intrauterine pregnancy;2(3.3%) had spontaneous abortion,and 2(3.3%) showed ectopic pregnancy;the other 22(36.1%) had no pregnancy.Conclusions Conservative treatment by laparoscopy is safe and effective for ectopic pregnancy.To avoid repeated ectopic pregnancy,?-hCG monitoring after the operation is necessary.

Objective To observe and analyze the effect of neoadjuvant chemotherapy on locally advanced non-small cell lung cancer tumor markers and lymphocyte subsets of different pathological type .Methods A total number of 40 NSCLC patients which received neoadjuvant chemotherapy and 20 normal people were selected in our study .To compare the differences of CEA ,CA-125 , CYFRA21-1 and lymphocyte subset in NSCLC pantients with adenocarcinoma or squamous cell carcinoma and the the differences of CEA ,CA-125 ,CYFRA21-1 and lymphocyte subset in patients with different clinical effect .Results (1) The CEA ,CA-125 ,CY-FRA21-1 in pantients with adenocarcinoma or squamous cell carcinoma were both higher than normal people .The CEA level in pa-tients with adenocarcinoma was higher than squamous cell carcinoma ,while the CA-125 and CYFRA21-1 in squamous cell carcino-ma were higher than adenocarcinoma .The CD3+ ,CD4+ ,CD8+ ,CD4+ /CD8+ in pantients with adenocarcinoma or squamous cell carcinoma both had obvious differences with normal people .But there were no obvious differences between patients with adenocarci-noma and squamous cell carcinoma .(2)There were 18 cases of CR+ PR while 22 cases of SD+ PD .The CA-125 and CYFRA21-1 were decreased in patients of squamous cell carcinoma with clinical effective while the CEA and CYFRA 21-1 were decreased in ade-nocarcinoma with clinical effective .The CD3+ ,CD4+ ,CD4+ /CD8+ increased and CD8+ decreased in patients of squamous cell car-cinoma or adenocarcinoma with clinical effective .Conclusion The tumor marker and lymphocyte subsets values had obvious differ-ences in adenocarcinoma and squamous cell carcinoma .Both tumor marker and lymphocyte subsets were useful in diagnosis .

Acute Disease ; Combined Modality Therapy ; Humans ; Pancreatitis ; therapy ; Pancreatitis, Acute Necrotizing ; therapy

Objective To observe the therapeutic effect of Pinggan Yishen Ditan Decoction (Liver-Calming Kidney-Boosting Phlegm-Flushing Decoction) for hypertensive obesity and its possible mechanism.Methods Sixty cases of hypertensive obesity were randomly divided into Chinese medicine group (Chinese medicine was added on the basis of treatment in control group) and control group.The treatment course lasted for 8 weeks.Blood pressure (BP),body weight,waist circum-ference(WC),waist-to-hip ratio(WHR),metabolism parameters,fasting insulin(FINS) and Leptin were observed and compared before and after treatment.Results There was significant difference in SBP,DBP,BMI,WC,WHR,FPG,TC,TG,HDL-C,LDL-C,FINS,Leptin and ISI after treatment in Chinese medicine group (P

Objective To investigate the degenerative changes in the inner rat retina after photic injury. Methods After 24 hour-dark adaptation, sixty Lewis rats were exposed in a ventilated green plexiglass chamber that transmitted continuous green light between 480-520 nm with an intensity of 900～1 000 lx. After 24-hour exposure, the rats stayed in darkness and were sacrificed after 1 day, 3,7 or 14 days. The neurons in the inner retina were marked by immunohistochemical technique and observed by light and electronic microscope. Results The apoptotic photoreceptor cells were noted after photic injury. The degeneration and decreasing number of rod bipolar cells were found after 3 days; the edema of horizontal cells occurred after 1 day but ameliorated gradually; decreasing number of amacrine cells was found after 1 day; sustained edema of ganglion cells and prolifeeration of the M?ller cells were found after photic injury. Pyknotic and edematous neruronal degenerations of inner retina were found in ultrastructural study. Conclusion The neurons in the inner retina as well as M?ller cells are involved in the degeneration after photic injury. Different neurons manifest different patterns of degeneration.

Objective To study the changes in the expression of GABA and its receptors in the dorsal horn of rat spinal cord induced by hind paw formalin injection. Methods Twelve healthy male SD rats weighing 300-325 g were randomly divided into two groups: (A) control group (n=6) and (B) peripheral inflammatory hyperalgesia group in which 5% formalin 50?l was injected subcutaneously into the plantar region of right hind paw to produce persistent pain(n=6). The animals were killed 24h after formalin injection. L_(4-6) segment of the spinal cord was removed. The number of GABA immuno-reactive cells was examined by immuno-cytochemistry technique and the exprpession of GABA_(A?3) and GABA_(B1) receptor mRNA in the spinal dorsal horn were determined by in situ hybridization. Results In control group(A), GABA immuno-reactive cells and GABA_(A?3) and GABA_(B1) receptor mRNA were observed throughout the lumbar spinal dorsal horn. The density of GABA immuno-reactive cells and GABA_(B1) receptor mRNA was highest in the superficial laminae Ⅰ-Ⅲ; while the GABA_(A?3) receptor mRNA was evenly distributed in the spinal dorsal horn. In group B, 24h after formalin injection there was a significant increase in the GABA immuno-reactive cells in the ipsilateral spinal dorsal horn. The expression of GABA_(A?3) and GABA_(B1) receptor mRNA were significantly increased in bilateral dorsal horn compared to group A. The three parameters were positively correlated and their locations in the spinal dorsal horn were not significantly different from those in group A. Conchusion GABA and its receptors may play an important role in nociceptive modulation.

Objective To evaluate the biomechanical characteristics of the long U-shaped hollow pedicle screw system for percutaneous fixation of thoracolumbar fractures.Methods Models of vertebral compression fracture were created by flexion in 8 fresh cadaveric samples of thoracolumbar spine.The models were divided into 2 equal groups according to the fixation system applied.In group A conventional pedicle screws were used while in group B long U-shaped hollow pedicle screws were used.The yield compressive strength,axial compression stiffness,bending stiffness,torque and torsional stiffness of all the fixated spines in the 2 groups were tested by the bio-mechanical electrical measurement and compared as well.Results The conventional pedicle screws and long U-shaped hollow pedicle screws showed no significant differences regarding yield compressive strength (3450.02 ± 110.03 MPa vs.3430.04 ± 102.12 MPa),axial compressionstiffness (407.52 ± 33.02 N/mm vs.395.04 ± 26.46 N/mm),bending stiffness (305.14 ± 26.53 N · cm/Degvs.295.12±23.80 N · cm/Deg),torque (6.45±0.25 N · m vs.6.25±0.21 N · m) or torsional stiffness (0.54±0.04N·cm/Degvs.0.52±0.03N·cm/Deg) (P＞ 0.05).Conclusion The long U-shaped hollow pedicle screw system can satisfy the biomechanical requirements for fixation of thoracolumbar fractures and can function as well as the conventional pedicle screw system.

The myelodysplastic syndrome(MDS) comprise a heterogeneous group of clonal disorders.A unique aspect of MDS is the notion that both bone marrow failure and the propensity toward development of acute leukemia coexist. Origin of abnormal clone with MDS occurs at the level of multipotent progenitors or above it. Recently some kinds of MDS were observed the abnormal clone has been potentially originated from normal CD+34 CD-38 Thy-1 + hematopoietic stem cells. The abnormal clones with different chromosomal abnormality have different distribution. With the propotion of abnormal clonality increasing MDS has aggravated. Coherent result will provide new insight into the clinical diagnosis, treatment and prognosis of MDS by strengthening the research of MDS clonality.