Fingersucking ; adverse effects ; Humans ; Malocclusion ; etiology ; therapy ; Malocclusion, Angle Class II ; therapy ; Malocclusion, Angle Class III ; therapy ; Orthodontic Brackets ; Orthodontics, Corrective ; methods ; Orthodontics, Interceptive ; methods ; Palatal Expansion Technique ; Time Factors ; Tongue Habits ; adverse effects ; Tooth Extraction ; Tooth, Impacted ; surgery

Humans ; Oral Surgical Procedures ; education ; methods ; Surgery, Oral ; education ; manpower ; trends

Animals ; Blood Platelets ; Cell Proliferation ; drug effects ; Dental Implants ; Fibrin ; chemistry ; history ; pharmacology ; therapeutic use ; Gingival Recession ; therapy ; History, 20th Century ; Humans ; Mesenchymal Stromal Cells ; cytology ; drug effects ; Osteoblasts ; cytology ; drug effects ; Sinus Floor Augmentation ; methods

Animals ; Collagenases ; metabolism ; Dental Bonding ; Dental Caries ; enzymology ; Dentin ; enzymology ; pathology ; Gelatinases ; metabolism ; Humans ; Matrix Metalloproteinase 14 ; metabolism ; Matrix Metalloproteinase 20 ; metabolism ; Matrix Metalloproteinase 3 ; metabolism ; Matrix Metalloproteinases ; metabolism ; Sclerosis

Dentists ; legislation & jurisprudence ; Discriminant Analysis ; Dissent and Disputes ; legislation & jurisprudence ; Expert Testimony ; legislation & jurisprudence ; Humans ; Liability, Legal ; Malpractice ; legislation & jurisprudence ; Quality Control

OBJECTIVETo evaluate the effect of Al2O3 particles sandblasting on the surface roughness, element composition and resin bond durability of zirconia ceramic.

METHODSSixty 2.5 mm thick computer aided design and computer aided manufacture (CAD/CAM) zirconia ceramic (Vita Inceram YZ) plates were fired, polished and cleaned. Half of polished ceramic plates was sandblasted with 50 µm alumina particles at 0.3 MPa for 20 s. The surface roughness of polished and sandblasted ceramic surface were measured by 3D-laser scanning microscope, and the surface element weight and atom ratio of the ceramic surface were measured by energy disperse spectroscopy (EDS). Then polished and sandblasted ceramic plates were randomized into six groups. In Group 1 and 2 the polished and sandblasted ceramic plates were bonded irrespectively with conventional resin cement (DUOLINK). In Group 3 and 4 the ceramic plates were bonded with resin cement containing MDP (Panavia F), In Group 5 and 6 the specimens were pretreated with silane coupler acitivated by MDP (Clearfil Ceramic Primer), then bond with Panavia F. The specimens of each test group were then divided into two subgroups, and to received shear test after 0 and 10 000 time thermal cycle. The data was analyzed by one-way ANOVA and independent t test.

RESULTSComparing with polishing, sandblasting reduced the oxygen atom and weight ratio of zirconia ceramic surface (P < 0.001), and increased the zirconium atom and weight ratio (P < 0.001), meanwhile increased the surface roughness (P < 0.001). The bond strength between ceramic plates and resin cement in all test groups decreased after thermocycling (P < 0.001). All specimen in test group 1 and 2 lost bond, and the bond strength of test group 3 and 5 [(0.59 ± 0.17), (0.89 ± 0.84) MPa] were significantly lower than that of test group 4 and 6 [(14.63 ± 3.03), (16.64 ± 1.90) MPa], and the bond strength of test group 6 were significanlty higher than that of test group 4.

CONCLUSIONSSandblasting improves durability of bond between zirconia ceramic and resin cement containing MDP, not only by increasing the roughness and area of ceramic surface, but also by changing its surface element composition to obtain more chemical bond.

Aluminum Oxide ; chemistry ; Ceramics ; chemistry ; Dental Bonding ; Dental Stress Analysis ; Materials Testing ; Microscopy, Electron, Scanning ; Resin Cements ; chemistry ; Shear Strength ; Surface Properties ; Zirconium ; chemistry

OBJECTIVETo investigate the effect of downregulation of N-cadherin expression on cell proliferation, cell cycle, cell apoptosis and cell migration in tongue squamous cell carcinoma cell line Tca8113 cells.

METHODSN-cadherin siRNA was transfected into tongue squamous cell carcinoma cell line Tca8113 cells and Tca8113 cells were divided into three groups: untreated group, control siRNA group and N-cadherin siRNA group. The cells were harvested 48 h after transfection with N-cadherin siRNA. Cell proliferation of Tca8113 cells was examined by cell counting kit (CCK)-8 after transfection with N-cadherin, and the effects of downregulation of N-cadherin on cell cycle and cell apoptosis of Tca8113 cells were investigated by flow cytometry. The effect of downregulation of N-cadherin expression on cell migration of Tca8113 cells was observed by Boyden chamber experiment, and further expression changes of gene-related cell proliferation, cell cycle and cell migration were detected by Western blotting.

RESULTSN-cadherin siRNA downregulated the N-cadherin expression and significantly inhibited cell proliferation of Tca8113 cells (P < 0.05). The results of cell cycle revealed that the percentage of G(0)/G(1) phase in N-cadherin group [(65.41 ± 0.92)%] was significantly higher than that in untreated group [(41.59 ± 1.43)%] or control siRNA group [(43.70 ± 2.08)%], and there was significant difference among the three groups (F = 216.839, P = 0.000). The percentage of cell apoptosis in N-cadherin group [(25.66 ± 1.36)%] was significantly higher than that in untreated group [(2.38 ± 0.14)%] or control siRNA group [(2.81 ± 0.12)%], and there was significant difference among the three groups (F = 850.364, P = 0.000). The cell number migrated into memebrane in N-cadherin group was significantly lower than that in untreated group and control siRNA group, and there was significant difference among the three groups (F = 140.858, P = 0.000). Further, compared with untreated group and control siRNA group, the expressions of matrix metalloproteinase (MMP)-2 and MMP-9 proteins were significantly downregulated and expression of p21 protein was significantly upregulated (P < 0.05).

CONCLUSIONSN-cadherin may play a role in occurrence and development of tongue squamous cell carcinoma.

Apoptosis ; Cadherins ; genetics ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Cycle ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Down-Regulation ; Humans ; Matrix Metalloproteinase 2 ; metabolism ; Matrix Metalloproteinase 9 ; metabolism ; Proto-Oncogene Proteins p21(ras) ; metabolism ; RNA Interference ; RNA, Small Interfering ; genetics ; Tongue Neoplasms ; metabolism ; pathology ; Transfection

OBJECTIVETo investigate the role of transcription factor special AT-rich binding protein 2 (SATB2) in the osteoblasts differentiation of bone marrow stromal cells (BMSC) in vitro.

METHODSRats bone marrow stromal cells were isolated by Percoll sedimentation and the cells were placed and allowed to attach for three times. After passages, expression plasmid pBABE-hygro-satb2 was constructed, then transfected into BMSC. BMSCs were inoculated in conditioned medium and osteogenic factors were detected by western blotting and reverse transcription polymerase chain reaction (RT-PCR).

RESULTSThe morphological observation of BMSC showed either spindle or polygonal pattern. The cellular phenotypic marker of the third passage was CD29 positive and CD34 negative. The growth curve possessed "S" pattern. The intensity of calfilication in BMSC was higher in SATB2 transfection group (IA value 125974 ± 241) than that in the control groups (IA value 178486 ± 406). Moreover, cell migration rate increased in SATB2 transfection group [width of scratch (0.72 ± 0.01) mm] compared with control group [width of scratch (0.83 ± 0.03) mm]. In addition, the mRNA expression of osteogenic factors runt-related transcription factor 2, Osterix, activating transcription factor 4, integrin-binding sialoprotein were upregulated.

CONCLUSIONSCells cultured with this method have general biological characteristics and osteogenic differentiation potential in vitro. SATB2 can promote osteoblasts differentiation of BMSC.

Activating Transcription Factor 4 ; metabolism ; Animals ; Bone Marrow Cells ; metabolism ; pathology ; Cell Differentiation ; Cell Movement ; Cells, Cultured ; Core Binding Factor Alpha 1 Subunit ; metabolism ; Integrin-Binding Sialoprotein ; metabolism ; Male ; Matrix Attachment Region Binding Proteins ; genetics ; metabolism ; Osteoblasts ; cytology ; Osteogenesis ; Plasmids ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stromal Cells ; metabolism ; pathology ; Thy-1 Antigens ; metabolism ; Transcription Factors ; genetics ; metabolism ; Transfection

OBJECTIVETo analyze the clinicopathologic features of salivary malignant pleomorphic adenoma (MPA) (the subtype of the malignant component was classified as non-specific adenocarcinoma).

METHODSThe clinical and pathological characteristics of 115 salivary gland tumors histologically diagnosed as MPA were analyzed.

RESULTSIn all the 65 MPA cases, there were 58 male and 7 female patients, and the mean age was 57 years (from 23 to 83). Sixty-one tumors were located in major salivary glands, and 4 in minor. Histologically the malignant components of 39 tumors were high-grade, 14 intermediate-grade, and 12 low-grade. Thirty-seven tumors were invasive carcinoma, 13 minimally invasive, and 15 non-invasive. The high-grade tumors had positive correlation with the invasive carcinomas (P < 0.05). The invasive carcinomas had positive correlation with TNM clinical stage (P < 0.05). The invasive carcinoma and the high-grade tumor had correlation with cervical lymph node metastasis (P < 0.05).

CONCLUSIONSNon-specific adenocarcinoma are the most common malignant subtype in MPA. The invasive and the high-grade types are more likely to metastasize to cervical lymph node.

Adenocarcinoma ; pathology ; Adenoma, Pleomorphic ; pathology ; Adult ; Aged ; Aged, 80 and over ; Female ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Staging ; Retrospective Studies ; Salivary Gland Neoplasms ; pathology ; Salivary Glands ; pathology ; Salivary Glands, Minor ; pathology ; Young Adult

OBJECTIVETo investigate the clinical natural course of temporomandibular joint (TMJ) intermittent closed lock (ICL) through 24 months follow-up.

METHODSSixty-eight patients with ICL were included, and 54 patients finished 24 months follow-up. The disease duration, frequency of joint lock and joint pain were recorded at the patient's first visit. Telephone interviews were taken for every month, and the frequency of joint lock and joint pain were recorded. According to the development of ICL, the patients were divided into 3 groups: symptom-worsened group, symptom-disappeared group, symptom-persisted group.

RESULTSThere were 16 patients (30%) whose symptoms worsened into closed lock (disk displacement without reduction), 32 patients (59%) whose symptoms persisted during the 24 months follow-up, and 6 patients' (11%) symptoms disappeared. In symptom-persisted group, the frequency of joint lock decreased in 11/32 (34%), increased in 4/32 (13%), did not change in 17/32 (53%). There was no significant difference in gender, age, frequency of joint lock and joint pain recorded at the first visit among these 3 groups (P > 0.05). The disease duration in the symptom-disappeared group was much shorter than the other 2 groups (P < 0.05).

CONCLUSIONSICL of TMJ was more likely to get worse into closed lock. There seemed no significant relation between the sequelaes of ICL and patients' gender, age, disease duration, frequency of joint lock and joint pain, and larger sample studies were necessary.

Adolescent ; Adult ; Child ; Disease Progression ; Facial Pain ; physiopathology ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Pain Measurement ; Remission, Spontaneous ; Temporomandibular Joint ; pathology ; Temporomandibular Joint Disc ; pathology ; Temporomandibular Joint Dysfunction Syndrome ; physiopathology ; Young Adult