Objective To test the protective immunity in mice induced by recombinant Schistosoma japonicum Sj14FABP through several adjuvant formulations. Methods The recombinant Schistosoma japonicum Sj14FABP was prepared by expression in E. coli as a GST fusion protein (rSj14/GST) and used to vaccinate outbred Kunming mice by using complete Freund's adjuvant (FCA)/incomplete Freund's adjuvant (FIA), Bacillus Calmette-Guerin (BCG) and the immunostimulating complex (ISCOM) as adjuvant respectively. Results The purified recombinant protein rSj14/GST was immunogenic in mice, and 34.3% and 36.0% worm reduction rates were obtained in outbred Kunming mice immunized intradermally with BCG adjuvant and immunized subcutaneously with ISCOM adjuvant respectively, compared with non-vaccinated control group. However, intramuscularly vaccination with rSj14/GST in FCA/FIA was not protective, although the high level of IgG antibody was induced. Conclusion Both BCG and ISCOM are suitable adjuvants for rSj14/GST.

Objective To describe rural people's perceptions of schistosomiasis and other dis eases, and to explore the relevance of these perceptions to disease control programs in rural areas of China. Methods A cross sectional study was conducted in Yingjiang Village, Anhui Province,China. Anthropological and epidemiological re search methods, including focus group discussions,in depth interviews, questionnaires and stool tests, were used. Results People identified various infectious diseases, such as schistosomiasis, hepatitis, hemorrhage fever, hookworm diseases, as cariasis and infection skin diseases. The knowledge of villagers regarding schistosomiasis and other diseases was not inconsiderable but not entirely accurate. Local illness taxonomy usually resulted from the concepts as to the causation of diseases. Local people thought there were about eight categories of factors that might cause diseases. These categories were worms, the unbalance of hot cold faetors, over work and starvation, "hot poison", dirty water and food, psychological factors, supernatural factors and pesticides. Conclusion Health education, improving sanitation and safe water supply may play much important roles in improving health of rural people.

The process of diagnosis,epidemiological survey and treatment of two cases of African imported quartan malaria is reported in this paper.

Objective To understand the trend and epidemiologic characteristics of malaria prevalence so as to provide the evidence for further control of the disease in Liaoning Province. Methods The data of registered cases of malaria in Liaoning Province from 2008 to 2013 were collected and analyzed with the descriptive epidemiological methods. Results A total of 202 ma-laria cases were reported from 2008 to 2013. There was no epidemic outbreak ,and the average annual incidence was 0.08/100 000. Totally 17 P. v. cases of local infection were reported,and 185 imported malaria cases were reported(P.v. 33 cases,P.f. 113 cases,P.v.&P.f. 13 cases,P.o. 3 cases,and unspecified 23 cases). The ratio of male to female was 5.91:1,the populations aged 31-45 years were the most(47.52%),and the main occupation was exported labors(46.04%). The 179 imported cases were infected in Africa and Southeast Asia,6 imported cases were infected in Anhui Province,Yunnan Province and Hongkong,and 16 local cases were infected in the border area of Dandong City. Conclusions Although the annual incidence of malaria in Liaon-ing Province is lower than 1/10 000,the incidence shows an increasing trend because the exported labors are increased. There-fore,the epidemic monitoring and health education should be strengthened,especially for the exported labors who have returned.

Objective To evaluate the clinical efficacy of sodium tanshinone IIA sulfonate(STS)in the treatment of ad-vanced schistosomiasis. Methods Fifty cases with advanced schistosomiasis admitted to the Touzao Township Hospital of Dong-tai City during the period from November 2012 to November 2013 were treated with STS for 10 days,and the internal diameter of the portal vein,levels of ALT,AST,γ-GT,PIIIP,CIV,HA and LN were measured and compared before and after the adminis-tration of STS. Results The mean levels of all serological parameters except HA were within the normal range before STS treat-ment,while the highest positive rate was detected inγ-GT(26.0%)and HA(54.0%). Following the STS treatment,the mean lev-els of all parameters and the positive rates reduced,with the greatest reduction observed inγ-GT(36.7%)and HA(37.8%);how-ever,the mean HA level was still higher than the normal range. The mean internal diameter of the portal vein reduced from(10.5± 1.7)mm before the STS treatment to(9.8±1.3)mm after the STS administration,with a significant diffrtence(P<0.05). Conclu-sion STS appears effective in the treatment of advanced schistosomiasis.

Objective To understand the status of elderly patients with schistosomiasis liver diseases combined with gallblad-der diseases,and explore the influencing factors. Methods A total of 280 elderly patients with schistosomiasis liver disease were divided into two groups,198 cases of chronic liver fibrosis and 82 cases of liver cirrhosis,and the results of their gallbladder ultra-sound and liver function examinations were analyzed statistically. Results Among the 280 cases,157 patients were combined with gallbladder diseases(56.1%),including gallbladder wall thickening(28.2%,79/280),cholecystolithiasis(13.6%,38/280),cholecystitis(11.1%,31/280),and gallbladder polyp(3.2%,9/280). The incidence rates of gallbladder wall thickening, cholecystitis and cholecystolithiasis in the schistosomiasis patients with cirrhosis were significantly higher than those in the schisto-somiasis patients with liver fibrosis(χ2=4.568,P<0.05). Conclusion The main influencing factors of schistosomiasis liver dis-ease combined with gallbladder diseases are the age,the course of the disease,liver cirrhosis and the portal hypertension degree.

Schistosomiasis is a neglected tropical disease that severely threatens human health and affects the socioeconomic development. The causative agent that parasitizes in humans mainly involves Schistosoma japonicum,S. mansoni,S. haematobi-um,S. intercalatum and S. mekongi. As the firstly identified schistosome,S. haematobium infection is found to strongly correlate with bladder cancer. This paper mainly reviews the discovery,morphology and life cycle of S. haematobium.

This paper reports a cecal hookworm patient whose main symptoms are dry cough,dyspnea and eosinophilia.

Objective To evaluate the effects of the schistosomiasis control mode(“Hubei mode”)that includes“compre-hensive measures in a whole endemic county”,“co-action of Health Ministry and Province”and“replacing cattle with machine”in Hubei Province. Methods The data of schistosomiasis control in Hubei Province from 2008 to 2012 were collected and a data-base including the annual schistosome infections of human and cattle,acute schistosome infection,outbreak of schistosomiasis en-demic,schistosome infected Oncomelania hupensis snails,and other prevention indicators was established and analyzed by using SPSS. Results Compared with 2008,in 2012,the infection rates declined by 64.91%and 88.63%in human and cattle respec-tively. The area with snails decreased from 5 423.85 hm2 in 2008 to zero. There were no acute schistosomiasis patients and out-break of schistosomiasis endemic. Conclusion The schistosomiasis control mode(“Hubei mode”)is effective significantly.

Objective To explore the risk factors of schistosomiasis cirrhosis esophageal varices hemorrhage. Methods A total of 113 cases of schistosomiasis cirrhosis esophageal varices hemorrhage and 128 schistosomiasis cirrhosis esophageal varices patients without hemorrhage were selected and their relevant factors were analyzed statistically. Results There were significant differences between the two groups in the prothrombin time,portal vein diameter,degree of esophageal varices,and varicose vein tumor-like lesions(P<0.01). Conclusion The risk factors of schistosomiasis cirrhosis esophageal varices hemorrhage are the prothrombin time,portal vein diameter,degree of esophageal varices,and varicose vein tumor-like lesions.