1.The clinical forensic identification of deep vein thrombosis
Yuli LIU ; Yijie ZHAO ; Guohui ZHANG
Chinese Journal of Forensic Medicine 2016;31(4):424-426
Deep vein thrombosis is the common complication of trafifc injury , it is one of the dififculties of forensic identiifcation to identify disability grade and the relationship between injury and disease. When post-thrombotic syndrome happens, circulatory system and leg will be dysfunction, caused by the destruction of valve and venous wall and by the blockage of thrombus. The quality of life and working ability will be seriously affected. The impact of different types of deep vein thrombosis on human body was analysised after looking up relevant literatures, then it was discussed that the identiifcation of following questions including: the relationship between injury and disease, how to identify disability grade and it’s best time.
2.Analyses of toxicology results in the ifre and non-ifre related carbon monoxide poisoning
Xuesong LU ; Feng LI ; Yao ZHAO ; Han ZHANG
Chinese Journal of Forensic Medicine 2016;31(4):384-386
Objective Explore the individual difference in carbon monoxide (CO) poisoning and analyze whether females were be more resistant to CO poisoning.Methods We collected 207 cases of CO poisoning deaths, and then we analyzed the Carbonyl Hemoglobin (COHb) results sorted by different sources of CO.Results The source of CO had significant impact on the fatal COHb level, but gender was not significant in affecting COHb level. In addition, it is possible that children and elders were less resistant to COHb than adults.
3.Forensic clinical significance of detecting the serum levels of IL-6 and TNF-αin patients with intracranial haemorrhage
Yunxiu BAI ; He BAI ; Dan CHAI ; Guoduong JIN ; Yujia CUI ; Wei WANG
Chinese Journal of Forensic Medicine 2016;31(4):379-381
Objective To explore the clinical significance of detecting serum levels of IL-6 and TNF-α in patients with intracranial hemorrhage.Methods The serum levels of IL-6 and TNF-α in different period were detected in patients with intracranial hemorrhage, by enzyme-linked immunosorbent assays (ELISA), and compared with those of healthy subjects (the control group).ResultsThe serum levels of IL-6, TNF-α in the severe and slight patients of study group on 1st, 3rd, 5th and 7th were signiifcantly higher than those in the control group (all withP<0.05). The serum levels of IL-6, TNF-α in the severe patients of study group were signiifcantly higher than those in slight patients of study group (all withP<0.05) on 5th and 7th. The serum levels of IL-6 and TNF-α in dead cases on 5th, 7th days admission were significantly higher than those in survival cases (P<0.05). The serum levels of IL-6 was positively correlated with TNF-α (r=0.721,P<0.05).Conclusion The detection of dynamically serum levels of IL-6 and TNF-α is of great clinical value for assessing the disease development therapeutic efifcacy and prognosis of brain injury patient with intracranial hemorrhage.
4.Comparative study of DNA extration of bloodstain on the iflter paper with four methods of solid phase adsorption
Huajie BA ; Jun MA ; Yanan LIU ; Aihua ZHU ; Ziqing LIN
Chinese Journal of Forensic Medicine 2016;31(4):369-372
Objective To discuss the effect of DNA extraction of bloodstain on the filter paper with four methods of solid phase absorption.Methods 180 bloodstain samples on the iflter paper, each one contains 1 microlitre anticoagulation peripheral venous blood, divided into 4 groups with 45 samples, respectively. All samples were treated with four methods of solid phase absorption, i.e. DNA IQ? System, D-shield sensitive DNA Extraction Kit, High efficiency Silica Bead DNA Extraction Kit and Conventional silica bead method. The concentration of DNA and the results of STR typing of four groups were compared each other.Results The concentration of DNA was 3.764±1.790μg/mL and 3.634±1.112μg/mL by using D-shield sensitive DNA Extraction Kit and High efifciency Silica Bead DNA Extraction Kit, respectively. However, the concentration of DNA by using Conventional silica bead method group (3.350±1.250) was not signiifcantly different from each other (P<0.05), while the concentration of DNA extracted with above three methods were higher than by using DNA IQ? System (1.864±1.207)(P<0.001); Signiifcant differences of peak height existed between DNA IQ? System and other three methods (P<0.001); As the same time, the peak height of samples by using High efficiency Silica Bead DNA Extraction Kit and Conventional silica bead method were signiifcantly different from D-shield sensitive DNA Extraction Kit (P<0.01).Conclusions The DNA extracted in bloodstain on the iflter paper by using D-shield sensitive DNA Extraction Kit, High efifciency Silica Bead DNA Extraction Kit and Conventional silica bead method was more than DNA IQ? System. Meanwhile, the quality of DNA using High efifciency Silica Bead DNA Extraction Kit and Conventional silica bead method may be higher.
5.The progress of abuse detection for three piperazines compounds
Jing CHANG ; Hongxia HAO ; Qinggang GAO ; Xiaoping HOU ; Guanfeng CUI ; Yunfeng ZHANG
Chinese Journal of Forensic Medicine 2016;31(4):360-363
Piperazine drugs, N - benzyl piperazine (BZP), 1 - (3 - chlorobenzene) piperazine (mCPP), 1 - (3 - three lfuorinated methyl phenyl) piperazine (TFMPP) have excitation and hallucinogenic effects similar to MDMA. They have became the alternative LSDs, abuse of substitues in the world reported increased year by year. The paper reviewed three piperazines regulation, abuse, toxicology, detection methods, and hope to provide a reference for judicial departments to crack down on the crime of the drug uses.
6.Genetic polymorphism study of the Y chromosome in pedigree with the surname Kong and their forensic implications
Yanan SUN ; Yi LI ; Dian ZHAI ; Yuming XING ; Faming ZENG ; Mao LI ; Baowen CHENG
Chinese Journal of Forensic Medicine 2016;31(4):341-344
Objective Acquiring genetic information of Y-SNPs and Y-STRs genetic makers from samples with the surname of Kong, which is useful for exploring the correlation between surname and Y chromosome in forensic applications studies.Methods Two multiplex genotyping assays and SNaPshot assay were used to analyze 255 unrelated male blood samples who share the same surname Kong and 330 unrelated male blood samples obtained randomly. 17 Y-STRs were typed for the surname Kong population samples. The software Arlequin 3.5.1.2 and the program Network 4.6.1.1 were used for data statistical analysis.Results 13 haplogroups were observed. The highest haplogroup frequency in the two populations were O3a2c1a-M117 (21.57%, 14.85%). 196 haplotypes in Kong population deifned by 17 Y-STRs locus were obtained and the haplotype diversity was 0.9939. 14-12-25-28-19-15-12-19-12-11-12-22-12-11-14-10-19 is the typical haplotype. Median Joining algorithm and Mismatch Distribution were adopted to analyze the Y-STR haplotye under haplogroup O3-M122, and the result shows that there are two “central star” distribution. Conclusion Combined with Y-SNP and Y-STR analysis showed that the Kong population had experienced complicated exchanges and expansion or continued growth, which has more than one surname origin. Hence, its population genetic structure and historical differences have potential applications in forensic science.
7.Graphical modeling of ICF Core Set (Comprehensive Version for Stroke)
Meng YOU ; Zhuqing JIANG ; Xu WANG ; Shengli DI ; Fengqin ZHANG ; Zhaoming GUO ; Jian XIANG ; Lin CHANG ; Tiantong YANG
Chinese Journal of Forensic Medicine 2016;31(4):336-340
Objective It aims to investigate the relationships among the categories of Comprehensive Version for Stroke as described in the International Classiifcation of Functioning, Disability and Health (ICF) Core Set, and to provide new supports for Judicial Appraisal of functioning in stroke by ICF functioning mapping.Methods The variables of 59 categories of ICF assessment scale and the samples of 106 persons’ are selected and used in the least absolute shrinkage and selection operator (LASSO) for mining dependencies among those variables. The graphical modeling and analyzing with the software Gephi provides a visual map of the correlations among those classiifcations. Results 59 interconnected categories which organized into the functioning mapping. b340, b735, b175 and b152 are centrally positioned categories because of their high correlation.Conclusion Functioning mapping by graphical modeling can reveal complex relational structures embedded in functioning classiifcations, which provides the support for using ICF to appraisal stroke.
8.Study on standardization methods in forensic medicine
Chinese Journal of Forensic Medicine 2016;31(4):330-335
Through analysis of the existing problems of standardization in the field of forensic medicine, combined with standardized methods in the course of evolution, applying the theory and method of standard system and complex standardization, and points out that the forensic medicine ifeld standardization methods should be according to the demand, through building standard system to guide the standardization work and to ifll standard missing items, improve the existing standards, and actively adapt to the need of development of multi discipline cooperation in forensic medicine, and promote the establishment of standard-complex.
9.The historical and cultural conditions of Xi Yuan Ji Lu,Song Ci--To commemorate the830th anniversaryof the birth of Song Ci
Chinese Journal of Forensic Medicine 2016;31(4):429-432
This article from the angle of history, culture, geography, legal,and personal conditions to discuss the generating condition of Song Ci and his Xi Yuan Ji Lu. Early development of forensic examination in our country was the historical conditions of Song Ci and his Xi Yuan Ji Lu. Zhu Xi Neo Confucianism inlfuence was the cultural conditions of Song Ci and his Xi Yuan Ji Lu. The development of political culture in the Southern Song Dynasty was the geographical conditions of Song Ci and his Xi Yuan Ji Lu. The inspection system of the Southern Song Dynasty was the Legal condition of Song Ci and his Xi Yuan Ji Lu. Make the Individual columns to systematic comprehensive was the result of Song Ci individual effort and summary .Therefor, Song Ci and his Xi Yuan Ji Lu appeared in the Southern Song Dynasty,which was the result of historical necessity and cultural heritage to a certain stage , even more the crystallization of Chinese culture and the cultural heritage of Forensic Medicine.
10.Medicolegal Analysis Based on the Investigation of 120 Rape Cases
Shu MOUHONG ; Baoqiang LI ; Hao ZHANG
Chinese Journal of Forensic Medicine 2016;31(4):376-378
Usually, the rape cases should include the evidence of both violence and sexual intercourse. These important evidences often were consist of mechanical injury, use of drugs and biological materials. Due to the rape pathway and the client behavior, there were some different showings in the type, feature, position of damage and also the biological species, legacy parts, legacy object etc. They were easily losed If ignored the process of collecting the biological materials. In this article, we summarized the elevant information and technical details related the ifnding, harvesting and testing of damage, drugs, biological examples. Meanwhile, we concerned the occurrence time, environment, regional, criminal methods, and the professional characteristics of suspects and victims. Hopefully the paper can give a hand to police.