Objective To confirm the difference in the biological characteristics between V?24 natural killer T cells(NKT) and the cytokine-induced killer cell(CIK).Methods V?24 NKT cells and CIK cells were expanded from human peripheral blood mononuclear cells.Purified TCRV?24~+ NKT cells and CD3~+CD56~+CIK cells were obtained by using Dynal beads.The phenotype and the levels of cytokine and necrosis-related factors expression on the purified NKT cells and CIK cells were determined by flow cytometry.The cytotoxicity of the purified NKT cells and CIK cells were measured by means of DIOC18 staining and flow cytometry.Results The proportions of TCRV?24~+V?11~+NKT cells and CD3~+CD56~+CIK cells were elevated up to 90% after purification.The majority of V?24 NKT cells were CD4~+ and DN NKT cells.They demonstrated high levels of expression of TCRV?24,V?11,CD3 and CD161 and a low level of expression of CD56,but most purified CD3~+CD56~+CIK cells were CD8~+ T cells,with high levels of CD3 and CD56 expression,low level CD161 expression and little TCRV?24 and V?11expression.After antigen stimulation,the purified CD3~+CD56~+CIK cells showed higher levels of expression of IFN-?,TNF-?,Perforin,FasL and TRAIL,compared to the NKT cells.CD3~+CD56~+CIK cells secreted little IL-4,whereas,V?24~+NKT cells secreted high level of IL-4.In addition,the cytotoxic effect of the CD3~+CD56~+CIK cells on tumor cell lines K562,U937 and Jurkat were more intense than that of the NKT cells.Conclusion It is evident that TCRV?24~+ NKT cells and CD3~+CD56~+CIK cells differ absolutely in many ways and might play different roles in anti-tumor immunity and immune regulation.

One hundred unrelated healthy indi- viduals of the Mulao nationality were ty- ped for HLA-A, -B, -C, -DR and -DQ locus antigens. The distribution of HLA class I antigens is similar to that of Zhu- ang, Miao and Yao nationalities in the Guangxi Area and to that of the Han po- pulations in southern China. For example, A2, A9 and A11 are much more frequent in A locus, as well as B40, B13 and Bw46 in B locus and Cw3. Cw1 (including Cx46) and Cw7 in C locus. The antigen B17 sho- wing a lower frequency in Mulao natio- nality than in other populations in sou- thern China is quite especial. The distri- bution of DR and DQ antigens in HLA class II shows still more different between Mulao and Zhuang nationalities. It is not clear whether the origin of the two na- tionalities or other factors give rise to their difference, and it needs further study.

A study of the mutagenieity of SAGS preservative solution for red cells,performed by Ames method, was reported.Result of the study in- dicated that there was no evidence of the positive mutagenicity reaction of SAGS preservative solution for red cells at its 6 doses ranging from 0.02 to 100mg/ml,whether the rat liver microsomal enzymes(S9)were added as metabolic activators or not.That is, the solution was unable to inducethe mutation at gene loci in the strains tested by Ames Method.

The absorption of anti-A and anti-B in reagent sera containing non-ABO antibodies,performed by coupling the ABH substance to se- pharose 4B,was introduced.The sera containing both ABO antibodies and anti-D(11),-C(5),-E(5),-c(6), -e(4),-M(1),-N(1),-s(1),-K(1), -Kp~b(1),-Fy~a(1),-Js~b(1)absorbed by this method,were tested then by the ABO-incompatible cells lacking relevant non-ABO antigens,and the panel red cells(O).As a result,all the ABO antibodies were absorbed exclusively,but all the non-ABO antibodies were preserved fullyThe non-ABO antibodies can't give the false positive reaction and their titer can't decrease after repeated absorp- tion.The advantages of this method and the factors that have influeced on effectiveness of the absorption were discussed.

A case of B, -D- blood groups was found in a Chinese woman whose baby died of haemolytic disease of newborn, Her husband was AB, CCDee blood groups. The unexpected antibodies in her sera were studied. The sera reacted with the red cells of all common Rh blood groups except -D-, (Rh)~(mod) and (Rh)~(null). Both respective and serial absorption and elu- tion tests were done for the ccDEE, CCD ee and ccde red cells. The results show that there are two antibodies in her sera. The anti-e is separable and the anti -Hr_o can react with all of the common Rh blood groups. The results were the same to that reported by Allen and Corocoran in 1958.

Effect of both granulocyte transfusions and antibiotics on the treatment of acute agranulocytosis with severe infections in 9 of 15 patients were reported. However, all 6 patients with agranulocytosis ind- uced by various drugs survived from sev- ere infections. It revealed that granulocyte count of each transfusion was more sign- ificant for the therapeutic effect.

Objective To investigate the circadian rhythm of peripheral blood cell counts in Chinese healthy volunteers and nasopharyngeal carcinoma patients. Methods A total of 13 nasopharyngeal carcinoma patients and 14 healthy volunteers were enrolled in this study. Venous blood was sampled every 4 hours during a 24-hour period. EDTA anticoagulated whole-blood was tested for blood cell counts immediately by automated hematology analyzer. The plasma cortisol level was determined by Radioimmunoassay. Data were analyzed by Analysis of Variance for Repeated Measures using SPSS 13.0 software and by Cosinor Analysis using Cosinor Analysis Software. Results Both healthy volunteers and nasopharyngeal carcinoma patients displayed clear circadian rhythms in red blood cells, hemoglobin, leukocytes, lymphocytes, and possibly platelets. Those blood cell counts also showed a significant variation during 24h. Conclusion Peripheral blood cell counts display significant circadian rhythms in both healthy volunteers and advanced nasopharyngeal carcinoma patients, suggesting the possibility of performing the time-selective collection and transfusion of blood.

Objective To investigate the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.Methods RT-PCR was used to study the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells in vitro. Results After coculture with leukemic bone marrow stromal cells, the expressions of CRIF1 and Gadd45 mRNA in Jurkat cells were significantly higher than the control.Conclusion High mRNA levels of CRIF1 might be associated with the G0/G1 phage arrest in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.

Objective To study the clinical effectives of red blood cell transfusion in patients with chronic aplastic anemia (CAA). Methods 425 CAA patients, from January 1995 to August 2007, were reviewed, and the rate of ineffective transfusions and its relationship to patients' age, sex, level of Hb before transfusion, times and volume of previous transfusions were analyzed.Results Of the 425 patients, ineffective transfusions occurred in 39 patients (9.2%). Of the 1 416 units of red blood cells transfused, 249 units (17.6%) were ineffective. The ineffective transfusions were not related to patients' age, sex or levels of Hb before transfusion, but were related to the times and volume of previous transfusions.Condusion In CAA patients, the more the times and the larger the volume of previous transfusions, the higher the rate of ineffective transfusion would occur.

Objective To explore the conditions for construction of human-to-swine hematopoietic chimera and its specific immune tolerance.Methods The human cord blood CD34+ cells (5?105/Kg) were transplanted to neonatal swine through intraperitoneal injections. The experimental swine, injected with human SCF (50ng/Kg.3d) and EPO (100U/Kg. 3d) simultaneously, and swine without injection were defined as transplantation group 1 and group 2, respectively. The swine, injected with saline, were taken as the control group. Peripheral blood (PB) was collected on day 10, 20, 30, 40, 50, and 60 after injection. The human CD71+cells in the chimera PB were quantitated by FACS and the genus-antibodies of PB were detected by blood group gel card. Results The percentage of human CD71+ cells in PB of transplantation group 1 was significantly higher than that of group 2 (P