A comparison between 70-blood-donor and 70-health-control blood lipoids,blood composition,and hemorrheological indexes by paired analysis method basedon occupation,sex and age was made.Paired T-test showed that though severalindexes of the blood donors reduced remarkably,they were still within the normalphysiological limits,There were no abnormal findings through physical examinationand ECG,indication that according to the general physique and present diet condi-tions of Chinese population.It is safe to donate 300ml blood once at intervals of three months.

A safe and effective method for separating albumin from autoplasma by heatwas introduced.Immunological analysis,showed that such an albumin solutionhad the same identity as human serum albumin.Determination by acetate cellu-lose electrophoresis showed the purity of the albumin was over 90%.Determination by polyacrylamide gel electrophoresis showed that one of thealbumin monomers was over 97%.Detection by double radial immunodiffusionindicated that there were no any new antigens in it.The recovery of the albuminwas more than 80%.

A specific monoclonal antibody(McAb),which could identfy different conformations of protein C(PC) in the presence or absence of Ca24+ .was selected and coupled with Sepharose-4B gel to form an affinity chromatographic column. When fresh plasma passed through this column.its PC would he adsorbed exclusively,and the obtained effuent was PC-deficient plasma (PCDP). The residual PC in the PCDP gained was

In the paper, 32 lots of intravenous immunoglobulin (IVIG)were tested by an animal model,the hypotensive test of rats. A linear correlation was found between prekallikrein activator (PKA)activity and hypotensive reaction if the inflow of human IVIG was made rapid (r=0.96,n=23). The preliminary observations indicated that it would be satisfactory that the PKA activity was below 35IU/ ml and the size of reduction in blood pressure was not 15% higher than that of control animal's level.The rat model can provide a method for safe usage of the intravenous immunoglobulin and reliable detection of and control over the residual PKA activity in blood products.

In this paper,were reported 72 seracon-taining HLA-DR,DQ antibodies from 106HLA-ABC antibody positive placenta seraby absorption of packed platelets from 33HLA specificities defined donors.Amongthem 26 DR with 13 specificities and 2DQwere suitable for typing sera.The DQw1was the first instance reported in China.

The effect of preserving the platelet concentrate prepared from whole blood in domestic PVC plastic bags, using a new platelet-preserving addition——acidified glucose nutrient solution(AGN), in the condition of horizontal rotation at 22℃ was studied. The results of study showed that the platelet concentrate stored in the AGN solution for five days has its platelet counts, pH value, aggregability and hypotonic shock response (HSR) even more better than those of the control platelet concentrate preserved in the normal ACD solution. In addition, the platelet concentrate incubated with fresh plasma for two hours has its aggresability and hypotonic shock response obviously recovered. The observation of the platelet concentrate under transmission electron microscope indicated that the platelets preserved in AGN solution kept their morphology completed, without any pseudopodium. And so the effect of preserving the platelet concentrate with AGN solution in domestic PVC plastic bas is simiar to that obtained by foreign laboratories with CPD solution in the platelet preserving plastic bags of second generation.

A technique of solid-phase platelet im-munoserological assay was devised to selectsuitable platelet donors for patients withrefractory idiopathic thrombocytopenic pur- pura(RITP).The suitable platelets act-ed as carriers to bind with vinca-alkalo-ids,and then were gived to the patientswith RITP.The technique showed clini-cally significant.

A human myeloma cell line(IBTCHM8310) obtained from the bone marrow ofa patient who was diagnosed as multiplemyeloma has been repropagated to datefor 270 generations within 50 months.The cells recovered from cryopreserva-tion in liquid nitrogen for 17 times,grewand reproduced well.The growth curve shows that themultiplication of the cells were slow onthe first two days,then went straightly upand culminated on the 6th and 7th days.Chromosome analysis was performed on96 cells on metaphases of 164th generationand it was between 63-69.There was stilla prominent mode 65,66.Of each 1?10~5/mlcells suspension,0.5ml was injected in tra-peritoneally to nude mice,Significant tu-mor masses appeared 3 weeks after injec-tion.Pathological section showed that thecells were identical to those of their pa-rent line.

The FactorⅫ(FXII or a-Ⅻa)in Cohn's FIV was adsorbed by diato- mite,and then the FXⅡ(or ?-Ⅻa) was activated into ?-Ⅻa(Hageman factor fragment,PKA,MW31000)by the co-adsorbed kallikrein with sub- sequent releasing it toabuffer solu- tion.Finally,the product was purified by DEAE-Sephadex A_(50).The activity of prepared PKA was 8 963IU/ml,and the preparation can lead to signifi- cant reduction in blood pressure in rats.

Objective To analyse the necessity and feasibility of HCV RNA detection for donor screening.Methods Plasma from 50 donors was mixed into a minipool, the nucleic acid was extracted by NucliSens Extractor and amplified by NASBA, the amplification products were detected by ECL. A performance control and a positive control were coupled with the samples through storage, nucleic acid extraction, amplification and detection.Results Two specimens were found HCV RNA positive in the 10000 anti HCV negative samples.Conclusion HCV RNA screening of plasma minipools by NASBA may prevent hepatitis C virus infection.