More and more therapeutic monoclonal antibodies(mAbs)are widely used for treating various illnesses such as cancer and autoimmune diseases due to the specific and efficient properties of mAbs.All of the currently licensed therapeutic mAbs are of the IgG class.Fc region of IgG has a N-glycan which is structural heterogeneity.The glycan on Fc region has many significant roles on the structure,the effector functions and pharmacokinetics of mAbs.The glycan of Fc region can be changed through genome editing and optimization of cell culture condition.This review presents an overview of the role of the various glycans in the effector functions of therapeutic mAbs and the impact of various parameters on glycosylation pattern of therapeutic mAbs.

Objective To compare the effect of traditional open surgery and laparoscopic repair of gastric perforation on postoperative pulmonary function.Methods Fifty patients were divided into two groups according to therapeutic method.Group A underwent traditional open gastric perforation repair(n=25).Group B underwent laparoscopic repair of gastric perforation(n=25).The pulmonary functions were examined at 1 day,3 days and 7 days postoperatively.Results All patients had different degrees of postoperative pulmonary function decline.After operation,the forced vital capacity(FVC)and forced expiratory volume in 1 second(FEV1)and vital capacity(VC)in group A were significantly lower than those in preoperation(P<0.05).The patients in laparoscopic operation group decreased significantly only on the first day postoperative.The postoperative pulmonary function of patients undergoing open surgery was significantly lower than that of patients undergoing laparoscopic surgery(P<0.05).Conclusion Laparoscopic repair of gastric perforation has less damage to the lung function than traditional open gastric perforation repair.Laparoscopic surgery plays an important role in the recovery of postoperative pulmonary function.

Objective To analyze the curative effect and adverse reaction of lansoprazole combined with droperidol thiadiazole melitracen treatment in patients with functional dyspepsia.Methods From December 2013 to December 2015 in our hospital and accepted treatment of 90 patients with functional digestive adverse as the research object,according to the random number table were divided into the control group and the observation group,45 cases in each group.The control group by oral administration of lansoprazole enteric coated capsules,the observation group taking oral lansoprazole and droperidol thiadiazole melitracen,course lasted for 4 weeks,efficacy and adverse occurrence was compared between two groups of patients.Results After 4 weeks of treatment,the observation group with symptoms score GSRS(postprandial fullness discomfort,early satiety,abdominal pain,epigastric burning sensation were lower than the control group with postprandial fullness discomfort early satiety,abdominal pain,epigastric burning sensation,and the difference was statistically significant(P<0.05); the patients in the observation group after 4 weeks of treatment,the total effective rate(86.7％)was higher than the control group(66.7％)significantly,and difference was statistically significant(P<0.05); symptoms of adverse reactions of two groups of patients in the process of drug use were mild and did not give special treatment,in a few days after the basic relief.Conclusion Lansoprazole combined with fluorine Pai Sai tons of Murray Song Xin can effectively eliminate or reduce the symptoms of functional dyspepsia patients clinical and no serious adverse reactions,it is safe in clinical application.

Objective To investigate the clinical efficacy of irbesartan hydrochloride and its effect on serum levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in patients with essential hypertension.Methods From 86 patients with essential hypertension who were treated in our hospital from July 2014 to September 2016 were randomly divided into experimental group and control group.43 patients in the control group were treated with irbesartan tablets.Group 43 patients were treated with irbesartan hydrochlorothiazide tablets,and the clinical efficacy,blood pressure and serum TNF-α,IL-6 factor and the incidence of adverse reactions were compared between the two groups.Results The effective rate was 93.02％in experimental group the total effective rate control group was 76.74％,the total effective rate of the experimental group was significantly higher than the control group,the difference was statistically significant(P<0.05).Before and after treatment,the systolic and diastolic blood pressure were higher in the experimental group and the control group,and there was no significant difference between the two groups.After eight weeks of treatment,Systolic and diastolic blood pressure were decreased,and the degree of decline in the experimental group was more obvious,the difference between the two groups was significant,statistically significant(P<0.05).There was no significant difference in serum TNF-α and IL-6 between the experimental group and the control group before treatment.After eight weeks of treatment,the serum TNF-α,IL-6 factor decreased,and the degree of decline in the experimental group was more obvious,the difference between the two groups was significant,statistically significant(P<0.05).The incidence of adverse reactions was 2.33％in 43 patients,43 cases of ankle edema in the control group,3 cases of dizziness and headache,2 cases of mild uric acid and two cases of adverse reaction.The incidence of adverse reactions was 16.28％,The incidence of adverse reactions in the experimental group was significantly lower than the control group,the difference was statistically significant(P<0.05).Conclusion The treatment of essential hypertension with irbesartan hydrochloride is obvious,and the serum TNF-α and IL-6 are decreased and the safety is high.

Objective To compare and analyze the clinical effect and adverse reaction of oral azithromycin,oral and intravenous erythromycin on the treatment of mycoplasma infection in respiratory tract.Methods In our hospital in January 2015～March 2016 year,clinical datas of 110 cases with mycoplasma infection in respiratory tract in children were analyzed retrospectively.The total cases were randomly divided into the oral azithromycin treatment group(38 cases),oral erythromycin treatment group(36 cases)and erythromycin intravenous group(36 cases).The therapeutic effects and the adverse reactions were evaluated in the three groups.ResultsThe effective rate in the oral azithromycin group was significantly higher than that of the two other groups.The time of fever fading,rales and cough disappearance and the hospitalized day in the the oral azithromycin group were significantly shorter than that in the other two groups.Serum IL-8,TNF-alpha and hs-CRP level and the adverse reaction rate in the the oral azithromycin was significantly lower than that in the other two groups.The differences of all above the indexes were statistically significant(all P<0.05),the above index in the oral erythromycin group and erythromycin intravenous group was no statistical significance.Conclusion Compared with different medication route of erythromycin,it can get better results which oral azithromycin was used on the treatment of children with respiratory tract mycoplasma infection,the adverse reactions rate is lower,is a safe and effective treatment.

Objective To analyze clopidogrel combined with aspirin therapy of acute cerebral infarction the safety and efficacy of micro brain hemorrhage patients.Methods Select from May 2015 and May 2015 months in the hospital for diagnosis and treatment of 76 cases of acute cerebral infarction combined micro cerebral hemorrhage patient study,observation group in the conventional treatment at the same time giving oral aspirin and clopidogrel,the control group in the conventional treatment at the same time,only to give oral aspirin.To observe the nerve function defect National institutes of Health stroke scale score(National Institute of Health stroke scale,NIHSS)and daily life activities ability pap index,determination of patients' blood plasma viscosity,whole blood low shear viscosity,AA induction and the platelet maximum aggregation rate induced by ADP(MARAA,MARADP),observation records during the treatment patients with adverse reactions,were analyzed.Results Compared with before treatment,the observation group and control group after treatment for 2 weeks,NIHSS score,score and blood related indexes were significantly improved,statistically significant difference(P< 0.05).Compared with control group,the observation group after treatment the NIHSS score,pap score and blood related indexes were significantly improved,total effective rate increased significantly,the differences were statistically significant(P< 0.05).Two groups of adverse drug reactions occurred situation is no statistical difference.Conclusion Clopidogrel in combination with aspirin can effective treatment in patients with acute cerebral infarction combined micro brain hemorrhage,and security.

Objective To evaluate the efficacy and safety of levonorgestrel combined with danazol capsules in the treatment of endometriosis patients.Methods Ninety patients with endometriosis were randomLy divided into experimental group and control group(45 cases).The control group was treated with danazol capsules.The experimental group was treated with levonorgestrel on the basis of the control group.The clinical efficacy and serum CA125,CA199,ovarian function,and ovarian function were observed before and one month and three months after treatment.Adverse reaction rate.Results After three months of treatment,the clinical symptoms of the experimental group were lower than the control group(P<0.05); serum CA125,CA199 levels decreased,compared with the control group,the test group decreased significantly(P<0.05); ovarian function recovery Compared with the control group,the difference was statistically significant(P<0.05).two group compared with the incidence of adverse reactions,the difference was not statistically significant.Conclusion Levonorgestrel danazol capsules treatment of endometriosis Bitter patients than the use of danazol capsules better treatment,higher safety,worthy of clinical application to promote.

Objective To analyzethe expression level of peripheral blood mononuclear cell Toll like receptor 4(TLR4)in patients with acute coronary syndrome(ACS)undergoing elective percutaneous coronary intervention(PCI),and intensive atorvastatin on Toll like receptor 4 in clinical treatment.Methods 100 cases of patients with ACS were selected in our hospital from May 2014 to May 2016 were randomly divided into control group and experimental group,control group were given atorvastatin treatment after admission,20mg/d PCI postoperative continuous,after one month still maintain dose of 20mg/d.The experimental group were given atorvastatin treatment after admission,40mg/d,PCI postoperative continuous,after one month reduced to 20mg/d.After admission and a course of treatment,the expression level of peripheral blood mononuclear cells TLR4 was detected by flow cytometry and the Gensini scoring system on coronary lesion degree.At the same time,50 healthy subjects were selected as normal group.Results Compared with healthy subjects,the peripheral blood mononuclear cells TLR4 level in ACS patients has been significantly increased,and the level in patients with unstable angina pectoris is far lower than the level increased in patients with acute myocardial infarction,triple branch lesions groups were significantly higher than them in single and double branch lesion groups,the difference between the three obviously,with statistical significance(P<0.01); According to stepwise multiple linear regression and othercorrelation analysis showed that ACS category and the severity of coronary artery stenosis,coronary artery stenosis score were positively associated with the levels of TLR4(P<0.05); After treatment for one month,the levels of TLR4,the expression of low density lipoprotein cholesterol and total cholesterol levels were significantly lower,high density lipoprotein Cholesterol levels were significantly increased,the differences were statistically significant(P<0.01); compared with the normal group,the experimental group peripheral blood mononuclear cells of patients with Toll like receptor 4 expression level,total cholesterol,low density lipoprotein cholesterol level in the experimental group is higher than the normal group,high density lipoprotein cholesterol increased significantly,comparison between groups the differences were statistically significant(P<0.01).Conclusion The degree of lesions and the occurrence of acute coronary syndrome in patients with peripheral blood mononuclear cells of Toll like receptor 4 expression level increase has certain effect,increase the dosage of atorvastatin can significantly reduce the ACS after PCI in patients with peripheral blood mononuclear cells of patients with Toll4 like receptor expression.

Objective To investigate the preventive effect of octreotide combined with netilmicin sulfate on postoperative infection in suppurative appendicitis and its effect on helper T cell 17(Th17)/regulatory T cells(Treg)imbalance.Methods 148 patients with acute suppurative appendicitis in Xiangshan Hospital of Traditional Chinese Medicine from August 2014 to May 2016 were randomly divided into observation group(n=74)and control group(n=74).All were underwent laparoscopic appendectomy surgery,the control group took netilmicin sulfate combined with ornidazole for anti-infection after operative,while the observation group added octreotide acetate subcutaneously.The levels of serum interleukin(IL)-4,IL-6,IL-10,IL-17,IL-23,transforming growth factor β(TGF-β),interferon γ(IFN-γ)and Th17,Treg cell ratio and expression levels of related transcription factorbefore and after treatment were detected,Th17/Treg change were analyzed.Results The levels of IL-4,IL-6,IL-10,IL-17,TGF-β were decreased,IFN-γ were increased after treatment(P<0.01),the improvement of those indicators in the observation group were supior than control group(P<0.05).Th17,Treg cell ratio and expression levels of related transcription factor orphan nuclear receptorγt(RORγt),Forkhead box protein P3(Foxp3)were decrease,Th17/Treg was reduced after treatment(P<0.01),those indexes in observation group were lower than those in control group(P<0.05).The wound infection,intra-abdominal infections and other adverse events had no significant difference in two groups.Conclusions Octreotide combined with netilmicin sulfate can prevent postoperative infection in suppurative appendicitis,regulate Th17/Treg imbalance,restore immune function,and no significant adverse reactions.

Objective To explore the the effect of Omeprazole combined with operation on treatment of rupture of small intestine in closed abdominal injury.Methods From June 2014 to December 2014,64 cases in Xinchang county people's hospitalwith rupture of small intestine in closed abdominal were divided into the treatment group and the control group,32 cases in each group.The control group were treat with surgery,the treatment group were given surgery combined with Omeprazole.The total effective rate,quality of life,the recovery time of bowel sound and the echaust time in the two groups were compared.Results The total effective rate(90.6％,29/32)in the treatment group was significantly higher than that in thecontrol group(75.0％,24/32),the difference is significant(P< 0.05).The life index in the treatment group is significantly better than that in the control group(P<0.05).The recovery time of bowel sound in the treatment group(15.3±2.6)h,is shorter than that in the control group(23.8±6.8)h; the exhaust time(23.6±2.2)h in the treatment group is lower than that in the control group(32.3±4.6)h,the differencesis statistically significant(P< 0.05).Conclusion It can significantly improve the effect and postoperative indexes which omeprazole combined with surgical treatment on the treatment of rupture of small intestine in closed abdominal injury,Itshould be widely promoted.