The government of North Song dynasty collated Inner Canon of Huangdi for five times.They were Plain Questions collated in Tian Sheng,Jing You,Jia You Zhi Ping,Huangdi Zhenjing collated in Zhe Zong,and Inner Canon of Huangdi collated in Hui Zong.The edition collated by LIN Yi lasted for several years had been extensively used for hundred years.Inner Canon of Huangdi collated by the government was mostly used for medicine,but the edition collated in Hui Zong was used for cultivation of taoist.

PEI Xue-yi has 60 years,experience in treating pediatric diseases with traditional Chinese medicine,especially obtains good effect in children's nephrapiathy.Based on the regulation and characteristics of children's zang-fu,yin-yang,blood-qi and children's nephrapiathy,he makes different therapeutic plans in different phases.In clinical treatment,he emphasizes the lung,the spleen and the kidney and combines syndrome differentiation with disease diagnosis.Eliminaing pathogens,excessive damp-heat is used in acute phase and strengthening healthy qi,securing the spleen and kidney is used in recovery phase.Children's nephrapiathy is treated by three phases as the edema,protein urine and rescovery stage.He adopts the methods of dispersing lung qi and invigorating spleen for diuresis,clearing heat-damp,nourishing spleen and kidney,nourishing yin for protecting lower jiao.

Objective:To observe the clinical curative e ect of treating diarrhea-predominant irritable bowel syndrome(D-IBS) with Lichang Tang(Intestine-Regulating Decoction) and sertraline hydrochloride.Methods:72 patients with(D-IBS) was randomly divided into 2 groups:32 cases in the treatment group was given self-prescribed Lichang Tang(modi ed Tongxie Yaofang [Main recipe for painful diarrhea]+Jinlingzi San [Toosendan Powder]) and sertraline hydrochloride(50 mg,p.o.once in the early morning);35 cases in the control group were given Lichang Tang alone for oral administration.During administering these drugs,other drugs were discontinued in the 2 groups.Psychological counseling and dietary modi cation were used in all patients.The course of treatment lasted for 4 weeks.Results:The total e ective rates in the treatment group and the control group were 86.49% and 65.71% respectively,and there was signi cant di erence(P

Objective:To establish a method for determination of total flavonoids,eriodictyol-7-methylether and isosakuranetin in Artemisia halodendron seeds.Method:The content of total flavonoids was determined by ultraviolet spectrophotometry at 506 nm,and compared with eriodictyol-7-methylether as a reference substance;the content of eriodictyol-7-methylether and isosakuranetin was determined by RP-HPLC,the analytical column was YMC-Pack ODS-A column(5?m,4.6 mm?250 mm),the mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous(35:65) at a ow rate of 1.0 ml?min-1,and the detection wavelength was 288 nm.Results:The linear range of total avonoids was 0.1228--0.7368mg(r=0.9991) and the average recovery(n=6) was 100.5%(RSD=1.41%);the linear ranges of eriodictyol-7-methylether and isosakuranetin were 3.016--30.16?g/ml(r=0.9999)and 3.304--33.04?g/ml(r=0.9999),respectively;the average recoveries(n=6) were 98.9%(RSD=1.20%)and 96.8%(RSD=1.38%),respectively.Conclusion:The methods of UV and HPLC are simple,accurate with good reproducibility and suitable for determination of total avonoids,eriodictyol-7-methylether and isosakuranetin.

Objective:To establish the HPLC fingerprint of Prunella Species from different places and evaluate it by cluster analysis.Methods:The analysis method of HPLC ngerprint was established.Alltima C18(4.6mm?250mm,5?m) was utilized for separation.The mobile phase consisted of methanol(A) and 0.5% glacial acetic acid(B) with a ow-rate of 1.0ml/min.The detection wavelength was 280nm and the injection volume was 20?l.The column temperature was 30℃.The HPLC ngerprints of Prunella Species from three di erent places were determined and analyzed by SPSS.Results:There is a big di erence among ngerprints of Prunella from di erent places.Prunella from Hubei,Anhui,Jiangxi,Guizhou,Guangxi,Sichuan were divided into a class;Henan,Jiangsu,Zhejiang were divided into another class.Conclusion:the eatsblished ngerprint showed characteristics of Prunella Species from di erent places obviously,and the attribute of Prunella Species can be distinguished e ectively by cluster analysis method.

Objective:To observe the effect of Schisandra Chinensis Baill distilled by ethanol on energy metabolism in brain of repeated cerebral ischemia-reperfusion model mice.Method:The mice were randomly divided into six groups,respectively irrigating the groups of Nimodipine suspension,ginkgo leaf suspension,and large and small doses of Schisandra Chinensis Baill distilled by ethanol,sham group(blank) and the model group for the same volume of saline,once a day,15 days for delivery.Model mice of cerebral ischemia-reperfusion injury was established by bilateral common carotid artery blocking.48 hours after operation,the rats were decapitated,weight half of the brain and put 9 times saline into it,then the level of LD,vitality of LDH,AchE and ATP were measured.Results:Compared with model group,small doses of Schisandra Chinensis Baill distilled by ethanol could markedly reduce the content of LD of mice of repeated cerebral ischemia-reperfusion,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of LDH;large and small doses of Schisandra Chinensis Baill distilled by ethanol could both notably improve the vitality of AchE,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of Na+-K+-ATP、Ca2+-ATP.Conclusion:Schisandra Chinensis Baill distilled by ethanol could markedly improve the energy metabolism of the model of repeated cerebral ischemia-reperfusion of mice.

Objective:To establish the quality standard of sinapine thiocyanate in Qingrehuashiyangyin granules by HPLC.Methods:HPLC was performed on Phenomenex C18 Column(250mm?4.6mm,5?m) with acetonitrile-0.08mol/L KH2PO4(15:85) as mobile phase.Flow rate was 1.0ml/min and detecting wave length was 326nm.Column temperature at 30℃.Results:The good linear relationship was obtained in the range of 0.3976--1.988?g(r=0.9998),and the average recovery was 99.0%.Conclusion:This method is simple,fast and accurate for the quality control of Qingrehuashiyangyin granules.

Objective:To probe e ect of a Chinese compound recipe of nourishing yin and clearing heat and prednisone on nuclear protein of splenic lymphocytes in mice.Methods:MRL/LPR female lupus mice were divided into four groups respectively treated with isotonic Na chloride,traditional Chinese medicine,Western medicine and integrated Chinese and Western medicine,then nucleoproteins of splenic lymphocytes were extracted and detected by SELDI-TOF-MS method.Result:There were some proteins in control group which aggravated autoimmune course,and some proteins in Western medicine group which rivalried immunological activity;integrated Chinese and Western medicine and Western medicine showed similarly e ect on nucleoprotein of splenic lymphocytes.Conclusion:The recipe of nourishing yin and clearing heat and prednisone can treat the disease by uencing in the di erentiation of splenic lymphocytes and the expression of proteins.

Objective:To approach the in unce of wild chrysanthemum on some neuroendoerine factors in ventricular remodeling induced by abdominal aortic banding(AAB)in rats.Methods:Myocardial hypertrophy,ventricular remodeling model was induced by abdominal aortic banding(AAB)in rats.After 35 days' treatment,the systolic blood pressure(SBP),diastolic blood pressure(DBP) and mean arterial pressure(MAP) were measured.Then the ratios of LVW/BW(1eft ventricle weight/ body weight),HW/BW(heart weight/body weight)were Calculated.The Angiotensin II(Ang II) content in heart tissue and the Aldosterone(ALD),Tumor Necrosis Factor(TNF-?)concentration of blood plasma were determined by radioimmunoassay.Results:The experimental data demonstrated that wild chrysanthemum could decrease SBP,DBP,MAP and the cardiac indexes of LVW/BW and HW/BW,significantly reduce the content of Ang II,ALD and TNF-?,decrease the Hydroxyproline content(P

Objective:To observe the therapeutic effect and mechanism of Huzhangyuzhuo decoction on treating chronic pelvic pain syndrome(CPPS).Methods:46 male Wistar rats were randomly divided into control group(n=10) and experiment group(n=36).The CPPS rat model was induced by intraperitoneal injection of diphtheria-pertussis-tetanus vaccine and subcutaneous injection of puri ed prostate protein with FCA suspension.Rats in experiment group were subdivided into model group,Chinese medicine group and western medicine group(n=12).After 30 days of treatment,pathology changes of prostate were observed,and levels of IL-1?,COX-2,PGE2,?-EP in prostate homogenate were measured by ELISA.Results:Huzhangyuzhuo decoction greatly reduced the in ammation of prostate and relieved the structure damage.At the same time,Huzhangyuzhuo decoction can decrease the level of IL-1?,COX-2,PGE2 and increase level of ?-EP.Conclusions:Huzhangyuzhuo decoction might treat CPPS by regulating of cytokines in the prostate,depressing in ammation and relieving structure damage.