Objective: To study the antiviral effect of polysaccharides from brown seaweed Lobophora variegata. Method: The crude polysaccharide was extracted with boiling water and precipitated with ethanol,and then fractionated with ion exchange chromatography. Its antiviral activity was tested by cytopathic effect (CPE) reduction assay and plaque reduction assay. The cytotoxicity of the water crude extract was determined by MTT method. Results: The crude water extract showed markedly antiviral activity against herpes simplex virus (HSV) type 1 and type 2 including ACV resistant strain and clinical strains with low EC50 values of 18.2 and 6.25 μg/ml respectively. It also showed very low cytotoxicity to Vero, HEp-2 and MDCK cells. Therefore, the crude extract possessed high selective index for antiviral effect. It also had some inhibitory effect on respiratory syncytial virus (RSV) but had no effect on influenza A virus. Two fractions were isolated from the water crude extract and they exhibited anti-HSV activity in the test of CPE reduction assay in Vero cells. Conclusion: Seaweed Lobophora variegata contains antiviral polysaccharides with high inhibitory effect on herpes simplex virus.

Objective: Lumiflavin fluorescence method was developed to detect the content of riboflavin in urine. Metheds: Based on decomposition of riboflavin into lumiflavin, fluorescence method was used to determine riboflavin in urine. Results and conclusion: This method was simple, fast, accurate and the relative SD was from 1.11% to 9.51%, and the recovery was from 92.8% to 100%.

Objective: To study the effect of photosynthetic bacteria (PSB) on serum lipids and antioxidant capacity. Methods: The rabbits were fed high lipid diet to induce hyperlipidemia. The effect of PSB on hyperlipidemia was studied with Spirulina platensis as the positive control. The content of serum TC, TG , HDL-C, SOD activity, and the content of GSH, LPO were determined. Results: PSB could significantly reduce the content of TC,TG , LDL-C in serum, increase H/L and decrease AI ratio. Meanwhile, PSB could improve antioxidant capacity, as greatly increase blood SOD activity, GSH in blood and liver, and reduce serum LPO. In comparison, PSB was better than Spirulina platensis. Conclusion: PSB can significantly regulate lipid metabolism and improve antioxidant capacity in hyperlipidemic rabbits.

Objective: The nutritional composition of the muscle of six kinds of cold-water fishes was analyzed . Methods: All fishes were collected and analysed from Bohai Experimental Farm, a base of Heilongjiang River Fisheres Research Institute by conventional biochemical methods. Results: The content of crude protein, lipid and ashes in muscle of six fishes was 75.49%-91.51%, 1.21%-16.80%, 4.93%-6.61% respectively. The total content of amino acids was 69.89 %-88.58%, of 8 essential amino acids 29.61%-38.23%, of 4 delicious amino acids 27.20%-34.92%. All data demonstrated that there is relatively high protein content, comprehensive amino acids and high content of essential and delicious amino acids. Conclusion: The six cold-water fishes belong to super cultured ones involved in high nutritional and economic value and worth to explore for food study and stipulation of germplasmic standard.

Objective: To investigate six mineral element contents of rice bran and milled rice from three color rice cultivars (white rice, red rice, black rice). Methods: Select 36 typical cultivars and determine Se, Zn, Fe, Ca, Mn, Cu contents in their rice bran and milled rice with AFS-2201 atomic fluorescent spectrometer and AA-680 atomic absorption spectrometer. Results: 1. The six mineral element contents of rice bran were 2.58, 3.35, 10.04, 4.58, 12.79 and 6.59 times as those of milled rice respectively. 2. The Ca content of black milled rice was significantly higher than that of white and red milled rice. The Mn content in rice bran of white rice was significantly higher than those of black and red rice. Other mineral element contents were not significantly different. 3. For rice bran and milled rice of different cultivars, the contents of each mineral element in three color rices varied greatly. Conclusions: 1. The content of six minerals in rice bran was 5.66 times on average as that in milled rice in all cultivars of three color rice. 2. Eight cultivars of the highest content of each mineral in rice bran and milled rice were recommended for commercial use and human consumption.

Objective: To study the antioxidative and antilipemic effect of the flavonoids purified from pomelo (Citrus grandis L.Osbeck) peel. Methods: The flavonoids were isolated and purified by NKA-12 macroporous adsorption resin and the polyamide column chromatography, and preparative TLC and HPLC. DPPH radical scavenging potential and inhibition of human LDL oxidation in vitro were determined. The effects TG , TC, HDL-C and LDL-C were investigated in mice with hypercholesterolemia induced by egg yolk. Results: Three kinds of flavonoids antioxidants named P11, P12 and P2, deduced as hesperidin, naringin and eriocitrin, were purified from pomelo peel. The radical scavenging potential of P2, P11 and P12 were 52.5%, 37.8% and 39.0% respectively (P