Objective To establish a special determination method of astragaloside Ⅳin Xiyangshen Jinqiangui Mixture by HLPC-ELSD. Methods HPLC-ELSD was performed on Inertsil C18 Column(5 ?m,4.6 mm?250 mm),acetonitrile-water(30 ∶70) served as mobile phase ,column temperature was 35 ℃,the flow rate was 1.0 mL?min-1,and an evaporating light-scattering detector (ELSD) was used as detector. Results For astragaloside Ⅳ,the linear range was 0.424 4～2.122 ?g and the average recovery was 101.77 %with corresponding RSD being 2.49 %(n=6). Conclusion The method is simple,sensitive ,reproducible,accurate and can be used for the quality control of Xiyangshen Jinqiangui Mixture.

In our experiment it was found thatinsulin was able to significantlyenhance the response of the thymo-cytes treated with nylon wool toConA in the presence of exogenousIL-1.It had been demonstrated thatinsulin was able to augment the pro-duction of IL-1 by macrophagesstimulated by LPS.These resultssuggest that the regulation of insulinon T cell activiation be associatedwith macrophages or IL-1 andinsulin play a regulating rolein other reactions mediated withIL-1.In addition,it was alsodemonstrated that the action of activeinsulin could be weakened if inac-tive insulin be not removed.

Objective: To study the effect of micronutrient fertilizer ratio on the growth and yield of aconite daughter root (the processed daughter root of Aconitum carmichaelii). Methods: Taking A. carmichaelii (Ranunculaceae) as test material, to study the growth and yield of aconite daughter root under the various ratio of Zn, B, Fe, and Mn by the uniform design. Results: To promote the growth and improve the yield of aconite daughter root, the optimum ratio of fertilizer Zn-B-Fe-Mn was 4:1:1:2. Under this ratio, the size of the tuber, the number of the fibrous roots, the length of fibrous roots, the volume of the sub-root, and the dry weight of the tuber, the fibrous roots, and the mother root were significantly or very significantly higher than those under the other fertilizer ratio, while the plant height and the dry weight of leaf were lower than those in the control treatment, with the weight of fibrous roots and the mother roots increased by 24.5% and 25.9% compared with those in the control treatment. The results also showed that there existed negative correlation between the plant height and the yield of per plant, while the dry weight of the fibrous roots and the mother roots showed significantly positive correlation with the yield of per plant. Conclusion: The above mentioned ratio of Zn-B-Fe-Mn as 4:1:1:2 is the best, since it could promote the growth and improve the yield of aconite daughter root more than the other treatment.

0.05). Compared with controlled central venous pressure (CVP) before and right after hepatectomy, CVP increased significantly (P

Objective To evaluate the clinical intubation of Bonfils intubation fiberscope in difficult airway of acromegaly patients.Methods Fifteen acromegaly patients who have one of the following criteria of preoperative airway assessment,Mallampati score ≥3,thyromental distance (≤6 cm),mouth opening(≤3.5 cm).After routine anesthetic induction the patients were intubated with Bonfils intubation fiberscope.Haemodynamic changes were observed and handling of the Bonfils intubation fiberscope was evaluated in terms of the ease of insertion into oropharynx,visualization of epiglottis,advancement into glottis aperture and slide down the tracheal tube.Intubating time and success rate were also recorded.Postoperatively,sore throat or hoarseness were followed up for all patients.Results After intubation systolic pressure and heart rate increased as compared with pre-intubation((P

The clinical data of 26 cases with pathologically confirmed primary gastrointestinal nonHodgkin's lymphoma (PGINHL) admitted to our hospital from January 2001 to December 2010 were retrospectively analyzed.Among 26 patients,12 cases occurred in stomach,9 cases in small intestine,4 cases in colon and 1 case in multiple sites.The most common symptoms were abdominal pain (73％),followed by gastrointestinal bleeding (23％) and abdominal mass (15％).The main pathological types were MALT lymphoma (35％),diffuse large B-cell lymphoma (35％),and enteropathy-type T-cell lymphoma ( 15％ ).There were 14 patients in stage Ⅰ,6 in stage Ⅱ,1 in stage Ⅲ and 5 in stage ⅣV.Radical surgery and/or chemotherapy was the main therapy for PGINHL.

Objective To observe the long-term efficacy of nasal endoscopy-guided high intensity focused ultrasound (HIFU ) in the treatment of allergic rhinitis .Methods 300 patients with allergic rhinitis who failed in medication were randomly divided into the treatment group(n=162) and the control group(n=138) .The patients in the treatment group were accepted the nasal endosco-py-guided HIFU therapy ,while the patients in the control group were subjected to the hypothermy plasma ablation .The two groups were followed up after operation ,and the visual analogue scale(VAS) was employed to evaluate the therapeutic efficacy at postoper-ative 3 ,6 ,12 ,24 months .The saccharin tests were performed at the beginning and ending of treatment to assess the nasal mucocili-ary function in the patients of the two groups who voluntarily accepted the functional test of nasal cilia .Results After 2-week treat-ment ,nasal congestion ,sneezing ,runny nose ,nasal itching and other symptoms in the two groups were significantly relieved with no obvious complication .2-year follow-up demonstrated that the total effective rate in the treatment group was 61 .4% ,which was markedly higher than 50 .7% in the control group(P=0 .017) .The average saccharin clearance time of the treatment group (n=49) and the control group(n=39) were(464 ± 152)s and(738 ± 149)s ,respectively ,and their difference was statistical significant (P=0 .026) .Conclusion The long-term efficacy of HIFU treatment in allergic rhinitis is superior to that of hypothermy plasma abla-tion ,possesses less impacts on the nasal mucociliary function ,and is worthy of clinical promotion .

Objective To investigate serum procalcitonin(PCT)and C-reactive protein(CRP)and clinical significance of changes in children with pneumonia.Methods 123 cases of childhood pneumonia included 48 cases of bacterial infection,mycoplasma infection in 36 cases,39 cases with viral infections,40 cases of physical healthy children were selected as the control group.They were measured in serum PCT and CRP content of the correlation between test results and analysis of clinical final diagnosis.Results The level of PCT in Bacterial pneumonia group, Mycoplasma pneumonia group and viral pneumonia group were (5.80 ±1.92)μg/L,(0.45 ±0.15 )μg/L and (0.32 ±0.17)μg/L,respectively,significantly higher than control group(0.19 ±0.10)μg/L(t =5.37,3.41,1.23, all P <0.05);the level of CRP in Bacterial pneumonia group,mycoplasma pneumonia group and viral pneumonia group were(45.34 ±13.35 )mg/L,(28.63 ±11.37)mg/L and (8.19 ±2.07)mg/L,respectively,significantly higher than control group(3.85 ±1.31)mg/L(t =5.37,3.41,1.23,all P <0.05);serum PCT sensitivity to detect pneumonia children,specificity and the positive predictive were 93.56%,82.35% and 69.25%,respectively,higher than serum CRP diagnosis of pneumonia sensitivity,specificity and positive predictive(χ2 =4.41,5.83 and 7.62, respectively,all P <0.05).Conclusion Serum PCT and CRP measurement can be used as an auxiliary to identify indicators of childhood pneumonia,the diagnosis of pneumonia in children has important clinical significance.

Through analysis of the general health status and resource allocation and utilization of health services needs of the state,the relationship between the two is discriminated,which suggests that we should strike a balance between the allocation of health resources and the health needs of the residents,to achieve the optimal allocation and utilization,to better serve the cause of development and the promotion of the health of the entire society,and improve health services and economic efficiency.

Objective To investigate the expression of VCAM-1 and E-selectin in vessel walls of the patients with thromboangiitis obliterans(TAO).Methods The vessel tissues were taken from 18 cases of amputation operation due to TAO and 16 cases of amputation operation due to non-vascular diseases.The expression of VCAM-1 and E-selectin were assessed by the immunohistochemical method.Results The positive expression rate of VCAM-1 in vessel wall of the patients with TAO was 77.8%(14/18),which of E-selectin was 72.2%(13/18);both of them were obviously higher than 6.3%(1/16) and 6.3%(1/16) in the control group.The positive rate of VCAM-1 and E-selectin had no correlation with the age,smoking amount,smoking time and drug use (P>0.05).Conclusion The expression of VCAM-1 and E-selectin have close correlation with the occurrence and development of TAO.Detecting the expression of VCAM-1 and E-selectin can serve as a diagnostic indicator of TAO.