Objective To identify the differentially expressed genes and functional enrichment pathways using bioin?formatics technology in mice who were acutely administered with Morphine. Methods First, we downloaded microarray da?tasets of mice which were acutely administered with Morphine from Gene Expression Omnibus, and assess the quality control parameters of the microarray datasets using AffyQCReport 1.42.0 package by R programming language 3.1.0 software. Subse?quently, the differentially expressed genes of the microarray datasets were identified using the linear models for microarray data of R language. Finally, functional pathways of the differentially expressed genes were enriched based on expression anal?ysis and systematic explorer. Results The microarray datasets showed preferable uniformity and intension similarity. A to?tal of 481 differentially expressed genes including Gm11627, Zfand4, Zbtb16, Pkp2 and Plin4 were identified. While 8 func?tional enrichment pathways, including pathways in cancer, melanogenesis and mitogen-activated protein kinase signaling were revealed. Conclusion The differentially expressed genes and functional pathways were the underlying biomarkers of mice who were acutely administered with Morphine.

Objective To investigate the value and limitations of ultrasound determination-evaluated brachial artery endothelial function in elderly diabetic patients. Methods High-resolution two-dimensional ultrasound was used to determine brachial artery(BA) flow-mediated vasodilation(FMD) during reactive hyperemia. Endothelial function was evaluated by FMD in 67 elderly patients with type 2 diabetes. Results In diabetes versus normal control group,the BA diameter and flow velocity showed no significant difference (P＞0.05) at basal state,but showed significant decreases (P＜0.05) after reactive hyperemia. Single-factor correlation analysis showed that FMD was in negative correlation with disease course, TG, ISI and age (r=-0.2357,-0.2215 and -0.3256, -0.2415,(all P＜0.05～0.01),and showed no correlation of FMD with BMI, TC, FBG, HbA1c, FIns. Conclusions FMD has a value in evaluating the flow-mediated endothelial dysfunction in older T2DM patients, while our analysis of the results should consider the patient's age, gender, race, disease course, blood lipids and other factors.

ObjectiveTo study the change of serum interferon- γ(IFN- γ ) and interleukin-4(IL-4)level in children suffered from cough variant asthma after montelukast combined with loratadine therapy.MethodsForty-six patients of children cough variant asthma were recruited and accepted montelukast combined with loratadine therapy for 8 weeks.The changes of serum IFN-γ and IL-4 before and after therapy were compared.ResultsCompared with before therapy,the level of serum IFN- γwas decreased [ (59.94 ± 9.43) ng/L vs.(39.94 ± 9.07) ng/L ] and IL-4 was increased[ ( 48.78 ± 22.23 ) ng/L vs.(60.78 ±16.48) ng/L] after therapy,there were significant differences (P ＜0.05).ConclusionMontelukast combined with loratadine in the treatment of children cough variant asthma can improve the balance of IFN- γ/IL-4,which deserve further study.

Objective To study the correlation of perceived social support and job burnout with subjective well-being of male nurses.Methods 122 nurses were selected from three large hospitals of Yantai and Weihai region,including 61 male and 61 female nurses,perceived social support scale,job burnout scale and subjective well-being scale were used for investigation.The correlation of perceived social support and job burnout with subjective well-being of male nurses was analyzed.Results Male nurse' occupation self-efficacy score as well as perceived social support score were significantly lower than those of female nurses.The three dimensions of job burnout of male nurses were negatively correlated with subjective well-being and perceived social support,perceived social support and subjective well-being.Perceived social support was positively correlated with subjective well-being.Job burnout and perceived social support could predict subjective well-being.Conclusions The social support and job burnout have a large impact on subjective well-being of male nurses.

The stem cell is a class of primitive cells with self?renewal,and multiple pluripotent potential capacities,which can differentiate into multiple specialized cells and generate human organs and tissues. Stem cell therapy is an interventional treatment that introduces new stem cells to damaged tissues,which facilitates the regeneration of trauma,disease and ageing?in?duced damaged human tissues to repair or replace the damaged tissues,thereby achieving the goal of disease treatment. It has been proved that the stem cell therapy is effective in the treatment of multiple human diseases,including cancer,diabetes,neu?rological disorders,autoimmune diseases,cardiovascular disorders and blood diseases. This review summarizes the advances in the research of stem cell therapy for human parasitic infections.

Objective To discuss the efficacy of ambroxol hydrochloride inhalation in treatment of infantile pneumonia.Methods Totally 80 pneumonia infants were selected and randomly divided into two groups.The control group (39 cases) were given anti-infection,cough-relieved,and other treatment.The observation group (41 cases) were given ambroxol hydrochloride inhalation on the basis of control group treatment.The efficacy of ambroxol hydrochloride inhalation in treatment of infantile pneumonia was evaluated by efficacy,clinical symptoms disappearing time,red blood cell hematocrit,blood viscosity,and adverse reaction during treatment.Results After treatment,the effective rate of observation group was higher than that of control group (P ＜ 0.05).There were 38 infants in observation group and 28 infants in control group with cough and dyspnea alleviated and phlegm reduced.After atomizing inhalation of ambroxol hydrochloride,the cough,pulmonary rales disappearing time and hospitalization time were significantly shortened (P ＜ 0.05).After treatment,the red blood cell hematocrit and blood viscosity of observation group was lower than that of control group (P ＜ 0.05).During treatment,there was no statistical significance on adverse reaction between two groups.Conclusion Ambroxol hydrochloride inhalation has a good curative effect on infantile pneumonia.It could improve the symptoms of pneumonia and reduce blood viscosity with good safety,which is worthy of clinical use.

Objective To study the effect of different insulin administration methods on glucose control of gestational diabetes mellitus ( GDM) .Methods Sixty-four cases with GDM were randomly divided into observation group and control group.The observation group received continuous subcutaneous insulin infusion insulin pump therapy, the control group received multiple subcutaneous insulin therapy.Then,the glucose control effect,SAS and SDS score were compared between two groups.Results After 4 weeks,the FBG,HbA1c,2h PG of observation group were significantly lower than the control group[(5.89 ±0.66)mmol/L vs (7.13 ±0.74)mmol/L,(7.14 ±0.82)% vs (8.92 ±0.95)%,(8.87 ±0.92)mmol/L vs (10.13 ±1.32)mmol/L](t=7.074,4.430,8.024,all P<0.05). The glucose fluctuations,insulin amount,hypoglycemia incidence of the observation group were significantly lower than the control group,blood sugar standard time was shorter than the control group[(5.64 ±0.65)mmol/L vs (8.44 ± 0.92)mmol/L,(0.52 ±0.06)U· kg-1· d-1 vs (0.73 ±0.09)U· kg-1· d-1,6.25% vs 28.13%,(4.76 ± 0.55)d vs (7.41 ±0.94)d,t/χ2 =14.061,10.983,5.379,13.764,all P<0.05)];SAS score,SDS score of the observation group were significantly lower than the control group[(34.95 ±4.2) points vs (40.23 ±3.98) points, (35.12 ±3.95)points vs (38.36 ±3.88)points,t=5.162,3.310,all P<0.05)].Conclusion Continuous subcu-taneous insulin infusion insulin pump therapy is helpful to control glucose levels,reduce blood sugar fluctuations and mitigate negative sentiment during treatment,it is an ideal insulin administration method for the treatment of GDM.

Objective To assess the value and complications of restaging laparotomy in women with presumed early ovarian cancer who have undergone inadequate initial staging procedures. Methods Between 1986 and 1996, 42 patients underwent restaging laparotomy in Cancer Hospital, Peking Union Medical College after receiving inadequate initial surgical procedure for presumed early ovarian cancer. Presumed stages from initial surgery include stage Ⅰa in 28 cases; Ⅰb in 1 case; Ⅰc in 12 cases; Ⅱa in 1 case. Histological distributions were as follows: epithelial cancer in 26 cases; malignant germ cell tumor in 9 cases; granulose cell tumor in 7 cases. Results Twelve patients (28.6%) had disease upstaged and eight (19.0%) had stage Ⅲ disease confirmed by restaging laparotomy. Positive findings at restaging laparotomy were mainly in omentum (16.7%) and pericolic gutters (33.3%). Peritoneal cytology was positive in 25.0 percent of patients. Three and five year survival rates for 30 patients with negative findings were 96.7% and 86.7%, respectively. Complications of restaging laparotomy included hemorrhage blood loss (50～ 1 100 ml, average 280 ml) and lymphocyst (7.1%). Conclusions Patients with presumed early ovarian cancer who had undergone inadequate staging procedures should undergo restaging laparotomy. Patients with negative findings have an excellent prognosis. Complications of restaging laparotomy were minor.

Objective To explore the effects of interest-induced and psychological intervention in pediatric operating room nursing.Methods Totally 150 cases of children undergoing surgery in our hospital were recruited and randomly divided into the experimental group(n=75)and the control group(n=75).The experimental group received routine care as well as interest-induced and psychological intervention in anesthesia induction room.The control group only received routine care.The children's anxiety degree,anesthesia induction cooperation and parent satisfaction were measured.Results The scores of anesthesia induction cooperation and anxiety in the experimental group were significantly lower than those in the control group (P＜0.05 for both),and parent satisfaction in the experimental group was significantly higher than that in the control group(P＜0.05).Conclusion The interest-induced and psychological intervention in pediatric operating room nursing can effectively alleviate preoperative anxiety,promote anesthesia induction cooperation and increase parent satisfaction.

Objective To investigate the expression and clinical value of platelet membrane glycoproteins CD 62p ,CD 63 in patients with type 2 diabetes mellitus (DM).Methods The levels of CD 62p ,CD 63 were measured by FCM in 64 cases of type 2 DM (31 cases with microangiopathy,33 cases without microangiopathy) and 30 normal individuals.Serial measurements were carried out in 16 patients before and after treatment.Results The levels of CD 62p ,CD 63 in type 2 DM were significantly higher than those in controls (P