OBJECTIVETo observe the effect of Qingre Yangyin Recipe (QRYYR) on sex hormones and insulin resistance (IR) in polycystic ovary syndrome (PCOS) patients.

METHODSTotally 90 PCOS patients were randomly assigned to the Chinese herbs group,the Western medicine group, the combined group, 30 in each group. Patients in the Chinese herbs group took QRYYR, one dose per day in two portions, once in the morning and once in the evening. Patients in the Western medicine group took Metformin 500 mg, twice per day for 3 consecutive months. Patients in the combined group took QRYYR and Metformin (the same as the former said two groups) in the 1st month, and took QRYYR for the following two months. Fasting blood glucose (FPG) and postprandial 2 h blood glucose (2 h GLU) were determined using hexokinase method before and after treatment. Fasting insulin (FINS), postprandial 2 h insulin (2 h INS), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), progesterone (P), prolactin (PRL), and testosterone (T) were detected using chemiluminescent method. Leptin and adiponectin (APN) were determined using ELISA. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. Body weight and height were measured once before treatment and once after treatment to calculate body mass index (BMI). The total two-phase basal body temperature (BBT) actually obtained within 3 months was statistically collected to calculate the two-phase BBT rate. Scores for Chinese medical syndromes were compared between the two groups before and after treatment.

RESULTSCompared with before treatment in the same group, BMI, FINS, 2 h INS, HOMA-IR, leptin, LH, PRL, T, and scores for Chinese medical syndromes obviously decreased, and APN levels increased (P < 0.05). FPG and 2 h FPG obviously decreased in the Western medicine group and the combined group (P < 0.05). E2 levels obviously decreased in the combined group with statistical difference (P < 0.05). Compared with the Chinese herbs group, the difference of BMI between pre-treatment and post-treatment was more in the combined group (P < 0.05). The difference of FPG,2 h GLU, 2 h INS, HOMA-IR, and APN between pre-treatment and post-treatment was more in the Chinese herbs group and the combined group (P < 0.05). Compared with the Western medicine group, the difference of PRL, T, and scores for Chinese medical syndromes was more in the Western medicine group and the combined group (P < 0.05); the difference of E2 and LH was even more in the combined group (P < 0.05). Compared with the combined group, the biphasic rate was obviously lowered in the Western medicine group (P < 0.05).

CONCLUSIONSQRYYR could improve IR but with weaker power to that of Metformin. It also could decrease serum levels of LH, T, PRL, and scores for Chinese medical syndromes, with superior effect to that of Metformin. The effect in the combined group was better.

Adiponectin ; Blood Glucose ; Body Mass Index ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Estradiol ; Female ; Follicle Stimulating Hormone ; Gonadal Steroid Hormones ; Humans ; Insulin ; Insulin Resistance ; Leptin ; Luteinizing Hormone ; Metformin ; Polycystic Ovary Syndrome ; drug therapy ; metabolism ; Progesterone ; Prolactin ; Testosterone

Objective]To observe and understand the effect and mechanism of QingReYangYin Recipe(QRYYR) on the ovary hormone level and mRNA expressions of PI3K and PKB in adipose tissue around womb and ovary in rat model of polycystic ovary syndrome(PCOS).[Methods]Rat PCOS model was established by Poretsky method and divided randomly into four groups: model group, western medicine group,low dose QRYYR group and high dose QRYYR group. Normal rats were used as normal group. All rats were orally administrated for 14 days and were weighed every day.After 14 days treatment, serum sample of each rat was obtained for analysis of estradiol(E2),progesterone(P), testosterone(T) contents. Each ovary was collected and weighed for measurement of the ovarian index. The fat pad around womb and ovary was obtained for analyzing the mRNA expressions of PI3K and PKB by PCR. [Results]Compared with the model group, the serum E2 and P levels in QRYYR group were significantly higher but T level was significantly lower(P<0.05) than the model group. The levels of E2, P and T in high dose QRYYR group had no difference from that in the western medicine group and the normal group(P>0.05).The ovarian index in the QRYYR groups was significantly decreased as compared with the model group(P<0.05),and that in the high dose QRYYR groups had no difference with the normal group(P>0.05).The mRNA expressions of PI3K and PKB were significantly increased in the QRYYR groups as compared with the model group(P<0.05),and the expressions in high dose QRYYR group, western medicine group and the normal group had no significant difference from each other(P>0.05). [Conclusion] QRYYR could significantly increase the content of E2 and P in the rat serum, significantly decrease the content of T and the ovarian index. Meanwhile, it could significantly increase the mRNA expressions of PI3K and PKB in adipose tissue. It could improve the ovarian function and decrease the local insulin resistance. The effect of the high dosage group on PCOS was superior or similar to Metformin.

Congenital Abnormalities ; etiology ; Deficiency Diseases ; complications ; Female ; Humans ; Maternal Nutritional Physiological Phenomena ; Pregnancy

Adult ; Burns, Inhalation ; diagnostic imaging ; Female ; Humans ; Male ; Middle Aged ; Radiographic Image Enhancement ; methods ; Radiography, Thoracic ; methods

Pneumonia is the primary cause of death in children under five-years old.About 2 million children younger than 5 years die of pneumonia each year worldwide,accounting for 19％ of the total number of deaths.According to the survey in 2008,7％ to 13％ of the children with pneumonia were severe pneumonia.Currently,the early diagnosis and treatment of severe pneumonia is often difficult.In order to improve the early diagnosis an prognosis cognition level of severe pneumonia,here is to make a review on the recent literatures on the biomarkers like brain natriuretic peptide,D-Dimer,C-reactive protein,lactate,zinc,procalcitoin,prealbumin,platelet count,endothelin-1 and high mobility group box 1 in children with severe pneumonia.

Basic English is the cornerstone that built the bridge between the world and the national treasure,the Traditional Chinese Medicine (TCM).With the increasing internationalization of the TCM,higher requirement is put forward by the society to the students as well as English teachers.In this paper,from the angle of students,author analyzes some problems in English teaching in the college of TCM.Meanwhile,author concludes some experiences from these years of teaching practices for solving those problems.

Objective:To study the feasibility of urinary iodine level at different times instead morningurine. Methods:We selected the urinaryiodine ofchildren aged 8~10 with stratified random sample method,collected the urine samples in the morning and at 10:00、12:30、4 pm and compared the difference of the urinary iodine level at different time. Results:There were significant differences between urinary iodine level in different time,different time interact with different age on the urinary iodine level,but do not with different sex by ANOVA with repeated measurement.There were no significant differences between morning Urinary iodine and at loan. Conclusion:There was the differences of urinary iodine between different ages,different time.It was feasible that use urinary iodine level by ANOVA with repeated measurement.T hter were NO significant differences between morning urinary iodine and at 10am.at 10am to replace the level of morning.

Children abdominal distention or bloating is one of the most common gut symptoms.It can be caused not only by gastroenterological diseases,but also by the illnesses beyond the gut.We discussed children abdominal distention here about causes,diagnostic clues,differential diagnosis,and treatment recommendations.All clinicians should carefully evaluate and cautiously treat abdominal distention.

Objective To investigate the factors associated with the academic achievement of the students with hearing impairment.Methods 110 undergraduates with hearing impairment were investigated with self-edited questionnaire, and their scores of Gaokao (University Entrance Examinations) were collected as the dependent variable. The correlation between the factors listed in the questionnaire and the scores of Gaokao was analysed. Results The personal factors of students (learning attitudes, motivation, learning experiences, psychological and emotional factors, etc.), the factors of teachers (development of teaching objectives, characters, professional knowledge, etc.), and the social economic factor of family, the action of parents for the education and the early intervention correlated with their academic achievement, while the gender and the degree of hearing loss did not. Conclusion The academic achievement mainly associate with the personal and environmental factors, such as the learning motivation, the early intervention, the teachers, and the parents, but not the body function, in the hearing impaired students.

The study aims to identify related substances in orlistat pharmaceutical substance from different production technologies. Advanced technologies including UPLC-QTOF-MS and UPLC-QTrap-MS were used and the structures or molecules of 10 kinds of related substances in orlistat were identified. The experimental data were valuable to the quality control and development of orlistat.
Anti-Obesity Agents ; analysis ; chemistry ; Chromatography, High Pressure Liquid ; Drug Contamination ; Lactones ; analysis ; chemistry ; Quality Control ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry