Pathological changes of Kupffer cells in number and ultrastructure were studied by means of colloidal carbon uptake, endogenous peroxidase cytochemistry, quantitative histology and ultrastructural stereologic morphometry at various time intervals (3,7,14,21,and 28 d) after common bile duct ligation. Kupffer cell phagocytic function and serum fibronectin opsonic activity were measured. The results show the morphological changes of Kupffer cells coinciding with their functional alterations. Alteration of Kupffer cell phagocytic function is mainly due to the changes of number and cellular activity of Kupffer cells.

Objective To explore the relationship between serum hs -CRP,IL -17 levels and senium type 2 diabetic macrovascular complications and its clinical significance.Methods The serum levels of hs -CRP and IL-17 were measured with immunoturbidimetry and ELISA respectively in 80 cases of type 2 diabetic patients with macrovascular complications,80 cases of the absence of any major vascular complications in patients with type 2 diabetes and 80 healthy subjects as the control.Results The serum hs -CRP and IL -17 levels of senium type 2 diabetic Patients with macrovascular complications were significantly higher than those of patients without macrovascular complication and healthy control group(t =19.18,23.14,all P <0.01);And serum hs -CRP and IL -17 levels of seniumtype 2 diabetic patients without macrovascular complications were also significantly higher than those of the healthy control group (t =23.64,19.97,all P <0.05 );While serum hs -CRP and IL -17 levels in different subgroup of type 2 diabetic patients with macrovascular complications were no significantly statistical significance(F =1.08,1.49,all P >0.05);In patients of type 2 diabetic Patients with macrovascular complications,hs -CRP was positively correlated with serum IL -17(r =0.712,P <0.05).Conclusion Hs -CRP and IL -17might be involved in the process of inflammatory response to diabetic macroangiopathy,and may have important role in the pathogenesis of senium type 2 diabetic patients with macrovascular complications.Detemination of hs -CRP and IL -17 levels in type 2 diabetic patients may be useful for early detecting diabetic macrovascular complications and serve as the index in monitoring the state of diabetic macrovascular complications.

Select 106 pregnant women with threatened preterm labor between 28 and 37 weeks of gestation. They have been treated continually by the preventing preterm labor therapy including antibiotics and magnesium sulfate. Cervicovaginal fetal fibronectin (fFN) and C-reactive protein (CRP) in serum were detected before treatment and after 3-day and 7-day treatment respectively. 100 normal pregnant women were included as control group. (1) The fFN and CRP had significant differences between study group and control group (P<0.05). (2) The fFN and CPR were different compared in the treatment period (P<0.05). Fetal fibronectin and CRP can be used can be used in therapeutic effect evaluation of threatened preterm labor.
C-Reactive Protein ; analysis ; Female ; Fibronectins ; blood ; Humans ; Infant, Newborn ; Obstetric Labor, Premature ; diagnosis ; prevention & control ; Pregnancy

Objective: To study the lung tissue TLR4 expression and airway hyperresponsiveness(AHR) in the mice infected by respiratory syncytial virus(RSV).Methods:16 clean,6 weeks old BALB/c mice were divided into two groups equally(RSV group and control).One week after RSV infection,AHR was detected by inhaling the increased concentration of methacholine,TLR4 mRNA expression was measured by RT-PCR methods,and the correlation between AHR and TLR4 expression was also analyzed.Results:An important difference was detected in two groups' airway resistance when the inhaled concentration of methacholine was 3.2g/L(P

Objective To study the treatment of postoperative pancreatic fistula. Methods The study (retrospectively) reviewed the management of 20 patients with pancreatic fistula. Fifteen patients were treated (conservatively), and 5 patients were treated surgically. Results All of the patients treated conservatively or by (surgery) were cured,and the results were satisfactory. Conclusions The majority of pancreatic fistulas can be cured with conservative management. If the fistula communicates with the main pancreatic duct or fails to heal after 5 months of conservative therapy, operation is necessary.Pancreatic fistulojejunostomy is a simple, safe and effective method.

Objective To study the clinical values of neuron-specific enolase(NSE) as serum tumor marker in lung cancer.Methods The serum NSE levels in 96 patients with lung cancer,60 patients with begnign pulmonary disease and 60 healthy controls were measured by using enzyme-linked immunosorbent assay.Results The levels of NSE in serum in patients with lung cancer was significantly higher than those in healthy subjects and patients with begnign lung disease(P0.05).The effective rate of chemotherapy were 87.10%,40.00% and 66.67%, 33.33% in patients with SCLC and NSCLC,respective for NSE positive and negative ones(P

Objective To discuss the clinical effect of sublingual misoprostol on planned delivery with full-term pregnancy and influence on outcomes of pregnancy and newborns.Methods Ninety-six puerpera with cervical bishop score ≤ 4 were randomly divided into the control group(n =48) and research group (n =48).The patients of the control group were given oxytocin therapy while the research group were given sublingual misoprostol.The promoting effect of cervical maturity,induced abortion effects,outcomes of pregnancy and newborns between the two groups were compared.Results The total effective rate of cervical maturity of research group was obviously higher than that of control group,the differences were statistically significant (91.7％ (44/48) vs 66.7％ (32/48),x2=7.834,P =0.015).The total effective rate of induced abortion effects of research group was obviously higher than that of control group,the differences were statistically significant (93.8％ (45/48) vs 54.2％ (26/48),x2=9.653,P=0.004).The postpartum hemorrhage((183.6±24.2) ml vs.(216.5±32.6) ml,t=6.748),total stageoflabor((7.6±1.3) h vs.(11.3±2.5) h,t=6.636) and cesarean section rate(31.3％ vs.47.9％,x2 =5.637) of research group was obviously lower than that of control group,the differences were statistically significant (P＜0.05).Two groups of newborns and the incidence of neonatal asphyxia,amniotic fluid pollution had no statistically significant difference(P＞0.05).Conclusion The clinical effect of sublingual misoprostol on.planned delivery with full-term pregnancy is obvious and causes little influence on outcomes of pregnancy and newborns.It is safe and effective to be applied on planned delivery and worth popularization and application.

Objective To investigate the correlation between CD40-1C/T gene polymorphism and serum soluble CD40L (sCD40L) expression in cerebral infarction.Methods According to the inclusion and exclusion criteria,select the acute large artery atherosclerosis in patients with cerebral infarction as the case group,select the same period without a history of stroke examination subjects as the control group.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology was used to detect CD40 gene polymorphism and sequencing,double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum sCD40L.Results A total of 209 patients with large artery atherosclerotic cerebral infarction (case group) and 87 subjects without history of stroke (control group) were included.The CC genotype (31.6％) and C allele frequency (53.8％) of the case group were significantly higher than that of the control group (17.2％,39.1％) (x2 =6.94,10.69,P ＜0.01).Case group serum sCD40L expression level was significantly higher than those in the control group [(3.97 ± 1.20) vs (2.69 ±0.88)] (t =10.19,P ＜0.001).Case group serum sCD40L expression level was different among CC,CT,and TT genotypes (F =19.22,P ＜0.001),serum sCD40L level (4.55± 1.16) of CC genotype in patients was significantly higher than that of CT (3.93 ± 1.17) and TT genotypes (3.27 ± 0.90),serum sCD40L level (3.93 ± 1.17) of CT genotype in patients was significantly higher than the TT genotype (3.27 ±0.90).The serum sCD40L expression level of the control group in the CC,CT,TT has no statistically significant differences among various genotypes [(2.91 ±0.79),(2.67 ±0.89),(2.61 ±0.91),F =0.619,P =0.541).Conclusions CD40-1C/T gene polymorphism is associated with serum sCD40L expression level in cerebral infarction,serum sCD40L level of the CC genotype was significantly higher.

Hepatitis B virus ( HBV) serological markers are common indicators for clinical diagnosis of hepatitis B.Qualitative anajysis of the markers is usually used in the diagnosis of HBV infection.while quantitative analysis is helpful to predict virological response of anti-virus therapy.Sometimes HBV serological markers will give unusual serological profiles,which make technicians and clinicians confused.This review discusses how to correc(t)ly report and explain the results of HBV scrological markers and how to apply the markers correctly for clinicians to analyse HBV infection status.

Modern medicine has entered the era of personalized medicine,the most mature of which is based on analysis of the specific gene polymorphism and tumor-related gene mutation and expression detection.Usually the test is related to the PCR technique such as real-time PCR,PCR-sequence,PCR-hybridization (on chip,membrane,latex particles),which leads to the gene amplification process.Now,some laboratories do or will to conduct such clinical tests.But most of them are non-classic clinical laboratories,which belong to the pathology,pharmacy or oncology department.There is a problem in front of us face that is how to standardize the clinical test on the key point of the test including design of the laboratory,collection and handling of the samples,quality control of testing procedure in the laboratory and report and results explanation for personalized testing and establish quality assurance system.