Environmental Monitoring ; methods ; Environmental Pollutants ; analysis ; Microchip Analytical Procedures

Consumer Product Safety ; Drinking Water ; standards ; Equipment Safety ; Food Safety ; Public Health ; instrumentation ; methods ; Safety Management ; Water Supply

The enzymological characterizations of site-mutagenized rat recombinant DNA polymerase?, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes were increased. Km values for the substrate (dTTP)of the wild enzyme, RQ182 and RQ183 were determined as 38.5, 34.5 and 111.1 ?mol/L, respectively,and the Km values for the primer (oligo(dT)) were 1.28, 1.96 and 6.58 ?g/ml, respectively. The results showed that the affinities of RQ183 to the substrate and the primer were decreased dramatically. It is suggested that Arg182 and Arg183 were involved in the active site function of DNA polymerase ?, in binding to DNA template, in recognizing of primer, and in binding to and catalyzing of substrates of the enzyme.

Objective:To prepare amphotericin B(AmB)-polybutylcyanoacrylate(PBCA) nanoparticle and evaluate its characteristics,so as to screen for the optimal preparation technique.Methods: AmB was loaded to blank PBCA nanoparticles by incubation to prepare AmB-PBCA nanoparticles.The surface of the nanoparticles was modified with polysorbate 80.The AmB concentration of AmB-PBCA-NP solution was calculated with the standard curve of AmB at D_(405),and the stability of solution was assessed.The diameter distribution of the particles was determined with Coulter Laser granulometer.The colloid solution of AmB-PBCA-NP was centrifuged and the supernatant was harvested to determine the concentration of AmB.The encapsulation rate,drug loading rate,and in vitro drug release were measured.The optimized preparation recipe was screened using diameter,drug loading rate,and encapsulation rate of the particles as indices.Results: The prepared AmB-PBCA nanoparticle had a regular spherical or elliptic surface,with a mean diameter of(69.01?28.56) nm.The curve of standard AmB was linear within the range of 1.12-5.60 ?g/ml: D_(405)=0.163 4c+0.006 6(r=0.999 3).The average recovery of AmB-PBCA-NP was(99.93%,) showing the solution of AmB-PBCA-NP was stable within 12 h.The nanoparticles showed a sustained drug release in vitro within 24 h.The optimized recipe was: DextranT-70 stabilizer without sodium deoxycholate,with a mean encapsulation rate of 56.10% and a drug loading rate of 82%.Conclusion: The current method for preparing AmB-PBCA nanoparticles is simple and can meet the requirements of pharmaceutics.

Objective:To explore the clinical effect of the emergency surgical treatment of tracheobronchial injury.Methods:11 operative patients with tracheobronchial injury were studied and analyzed.Results:8 patients were emergcntly operated with thoracotomy through tracheobronchial repair.2 cases suffered left inferior pulmonary atelectasis after operation and they healed with sucking sputum through tracheal cannula and positive-pressure ventilation.Right subphrenic abscess occurred in 1 case,and the patient healed with exploratory laparotomy and drainage.There were no death and no bronchopleural fistula or bronchostenosis after operation.Conclution:The emergency tracheobronchial prosthesis through thoracotomy is an important treatment for tracheobronchial injury,which is safe and accompanied with less complications.

The prevalence of asthma in pregnant women appears to be increasing.Pregnant women with uncontrolled asthma may have an increased risk of adverse perinatal outcomes.Optimal management of asthma during pregnancy includes avoiding or controlling asthma triggers,individualized pharmacologic therapy,objective monitoring of lung function,and patient education.Inhaled corticosteroids are the preferred treatment for the management of all levels of persistent asthma during pregnancy.It is safer for pregnant women with asthma to be treated with asthma medications than to have asthma symptoms and exacerbations.The goal of asthma management during pregnancy is to optimize maternal and fetal health.

Objective:To compare the safety and efficacy of premixed insulin aspart 30 (BIAsp30) with premixed human insulin 30R (BHI30)used in twice-daily injection regimen in patients with type 2 diabetes. Methods:Sixty-eight patients with inadequate glycemia control by oral drug were randomly divided into two groups using twice-daily insulin for 12 weeks comparison of BIAsp30 and BHI30. We compared HbA1c,seven-point blood glucose and frequency of hypoglycemia in two groups. Results:Blood glucose after breakfast and after dinner were significantly lower in BIAsp30 group than in BHI30 group(P0.05),meanwhile no serious hypoglycemia happened in BIAsp30 group. Conclusion:Post-prandial glycemia control was significantly improved,without increasing the risk of hypoglycemia,when people with type 2 diabetes were treated in twice-daily injection regimen with BIAsp30,as compared with BHI30.

Objective:In order to observe the effect of avandia combined with insulin to treat patients with type 2 diabetes mellitus(T2DM). Methods:Seventy two patients whose blood sugar was not controlled well by hypodermic were randomized devided into two groups. Group A was administered insulin alone,Group B was administered insulin and avandia. Fast plasma glucose(FPG),blood sugar 2 hours following meals,HbA1c,plasm insulin and exogenetic insulin doses,etc. Results:FPG,blood sugar 2 hours following meals,HbA1c,plasm insulin level and exogenetic insulin dose were all decreased. Conclusion:Using avandia combined with insulin to treat patients with T2DM can lower blood sugar level of patients and improve insulin resistance. [

Objective:To observe the effects of Lipo PGE1 in Treatment of Diabetic nephropathy(DN).Methods:60 patients with clinical albuminuria were divided randomly into the treatment group and control group,receiving regular treatment.In addition,the cases of treatment group received treatment of PGE1 10?g daily for two weeks.The index of Scr and UAER were observed before and after treatment.Results:The Scr and UAER in treatment group were more decreased than those in control group.Conclusion:Lipo PGE1 is effective in treating Diabetic nephropathy.

When the patients with chronic renal failure need drug therapy,we must select the appropriate drugs and adjust the drug delivery methods and dosage according to the renal function of these patients,at the same time we have to consider the factors such as age,sex,weight,combined medication,damage of other organs and so on,the therapeutic drug concentration monitoring should be paid attention to.For patients with blood purification,if the drug mainly removed by the kidneys,adjustments of drug administration must be based on the molecular weight of drug,volume of distribution,protein binding rate,mode of dialysis and hemodialyzer.We must ensure the drug efficacy and reduce the adverse reactions of drugs at the same time in the patients of renal insufficiency.