Objective:To clone and sequence of 16S~23S r RNA intergenic spacer region of Proteus mirabilis and Acinetobacter lowffii for further identification of these two bacteria by means of molecular probe.Methods: The PCR universal clinical common pathogenic bacteria primers for 16S and 23S rRNA's conserved sequences were designed.Then the genomes of these two bacteria were amplified,T Vector clones were constructed with PMD-18 T Vector ad PCR products.The vectors were sequenced and the sequence was submitted to Genbank.Results: T Vector clones were constructed with products amplified by using universal primers;according to Blast analyses,the results of sequencing were determined as ISR sequences of the two kinds of bacteria.And the reports to Genbank were accepted.Conclusion: Genbank accepted the cloning and sequencing results of Proteus mirabilis and Acinetobacter lwoffii as new sequences.And these sequences can be used to identify the two bacteria by universal primers.

Endocrine therapy is one of the important treatments of adjuvant therapy among patients with positive hormone receptor breast cancer.In the endocrine therapy setting,5 years of tamoxifen therapy has been the standard treatment regimen for premenopausal patients.In recent years,this standard has been challenged.The results of many large randomized clinical trials have made a new choice for adjuvant endocrine therapy in premenopausal patients.In this paper,we review the present situation of endocrine therapy in premenopausal patients with positive hormone receptor breast cancer.

Innovations of English class teaching methods are considered crucial in the improvement of English class effectiveness.Many approaches have been practiced towards that goal.The essay mainly discusses the issue from the new RICH perspective, intending to spark new thinking about innovations of English teaching methods.

Objective To compare tension-free repair and traditional hernia repair of inguinal hernia in the elderly patients. Methods During the period from 2000 to 2001,84 aged patients of scheduled for repair of inguinal hernia were randomized to tension-free repair or traditional hernia repair. The patients were followed up for two years. Results Tension-free repair of inguinal hernia was a simple procedure causing less trauma and operative time, fewer complications and recurrence. Conclusion Tension-free repair of inguinal hernia is superior to traditional hernia repair in the elderly patients.

Objective : To revise the general self-efficacy scale. Methods : 3094 subjects from 7 different cities in China finished the general self-efficacy scale. Results : The result of Item Analysis showed that 19 of the scale met psychometric criteria. The scale's internal consistency was 0.81, split reliability 0.81. It had acceptable discriminant validity: subjects with higher scale scores also had significantly higher scores in every items than those with lower scale scores, and this scale could effectively differentiate the high and low level managers, it also could effectively differentiate the managers groug and workers group. Exploratory factor analyses indicated that the scale had one dominant factor. Conclusion : The general self-efficacy scale has sound psychometric properties for further research.

OBJECTIVE:To establish a simple method for drug bioequivalence test based on Excel function.METHODS:The statistical tests including ANOVA,two one-side test and 90% confidence interval were carried out using Excel statistical function.RESULTS:Drug bioequivalence test can be performed based on Excel,with results in line with those stated in Biopharmaceutical and Pharmacokinetic.Moreover,due to Excel's repeated calculation function,for retest,three kinds of test results were available rapidly only by replacing the data with others remained for calculation.CONCLUSION:Excel function can be used to calculate drug bioequivalence.

ion:Objective To study the effect of enteral nutrition on cardio-pulmonary function in elderly patients with chronic obstructive pulmonary disease (COPD) .Methods Fifty-eight elderly COPD patients were randomly divided into treatment group (enteral nutrition group ,n=29) and control group (free diet group ,n=29) .Their nutrition parameters (BMI ,serum total protein ,al-bumin and prealbumin) ,LVEF and pulmonary function (FEV1 ,FVC ,FEV1/FVC) were assayed before and after treatment .Results The BMI ,serum levels of total protein ,albumin and prealbu-min were significantly higher in treatment group after treatment than before treatment ( P<0.05) .However ,no significant difference was observed in these parameters in control group after treatment .The LVEF ,FVC ,FEV1 and FEV1/FVC were significantly higher in treatment group than in control group after treatment (P< 0 .05) .Conclusion Enteral nutrition in combination with conventional treatment can improve the nutrition and cardio-pulmonary function in elderly COPD patients ,thus shortening their hospital stay time .

With the emergence of new endocrine drugs and the development of research in resistance mechanisms,significant progress has been achieved in the endocrine treatment of breast cancer in recent years. Fulvestrant,the combination of endocrine drugs and the combination of targeted therapy with endocrine therapy will be effective treatments for postmenopausal women with hormone receptor positive in advanced breast cancer.

Despite solitary pulmonary nodule (SPN) is a common medical problem in the clinic,the managements can be quite complex.The aetiological agent is largely unknown.Making a definitive diagnosis for SPNs before the operation has been a clinical challenge.A major problem for clinicians and radiologists were to identify the nature of benign and malignant SPNs at an early stage,and let the patients receive therapies timely and effectively.At present,many clinical features and detect methods of SPNs have been applied for differentiating the nodules,but these are not fully to distinguish it.Recently,some progressions had brought qualitative improvements to the diagnosis and treatment of SPNs.Therefore,in this paper,the research status and progresses of SPNs were reviewed.

Aim To establish a gefitinib-resistant NSCLC cell line, and observe its pharmacological properties. Methods HCC827 cells were treated with hepatocyte growth factor( HGF) and increasing concen-tration of gefitinib to induce cell resistance. MTT assay was used to test cell viability and chemosensitivity. Clone formation and Edu staining were used to verify the inhibitory effect of gefitinib on cell growth. qRT-PCR and Western blot were used to assay the expres-sion of c-Met. Results The use of HGF greatly short-ened the induction time of HCC827GR resistant cells. gefitinib could significantly suppress cell viability, col-ony formation and cell proliferation of HCC827 , but had a weaker inhibitory effect on HCC827GR cells. HCC827GR overexpressed c-Met protein, and was highly sensitive to c-Met inhibitors. Conclusion The gefitinib-resistant HCC827 GR cells were induced suc-cessfully and efficiently. The growth of HCC827GR is dependent on the activity of c-Met protein, and it can be used as a phenotypic screening model of c-Met in-hibitors.