Preventive Effects of Green Tea (Camellia Sinensis var. Assamica) on Diabetic Nephropathy.
10.3349/ymj.2012.53.1.138
- Author:
Min Yong KANG
1
;
Yong Hyun PARK
;
Bong Sub KIM
;
Su Yeon SEO
;
Byong Chang JEONG
;
Jung In KIM
;
Hyeon Hoe KIM
Author Information
1. Department of Urology, Seoul National University College of Medicine and Clinical Research Institute, Seoul, Korea. hhkim@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Camellia sinensis var. assamica;
diabetic nephropathy;
prevention
- MeSH:
Animals;
*Camellia sinensis/chemistry;
Cell Line;
Cell Survival/drug effects;
Diabetes Mellitus, Type 2/complications;
Diabetic Nephropathies/*drug therapy/*prevention & control;
Disease Models, Animal;
Dogs;
Kidney/cytology/*drug effects;
Male;
Mice;
Mice, Mutant Strains;
Plant Extracts/*pharmacology;
*Tea/chemistry
- From:Yonsei Medical Journal
2012;53(1):138-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study aimed to evaluate the preventive effects of Camellia sinensis var. assamica (CSVA) on diabetic nephropathy in in vitro and in vivo models. MATERIALS AND METHODS: MDCK cells were incubated with 1 mM of oxalate with or without different concentrations of CSVA, then MTT and malondialdehyde (MDA) assays were performed to investigate the preventive effects of CSVA on oxalate-induced cytotoxicity and oxidative stress. Thirty male db/db mice were divided into three groups. Group 1 were fed AIN-93G ad libitum; group 2 were fed AIN-93G mixed with 10% fermented CSVA ad libitum; group 3 were fed AIN-93G mixed with 10% non-fermented CSVA ad libitum. The mice were sacrificed 14 weeks later, and the serum glucose level, 24-hour urine chemistry, and morphological changes in the kidneys were examined. RESULTS: As CSVA concentrations increased, viable MDCK cells increased in concentration. MDA production decreased over time in the CSVA treated group. The creatinine clearance of group 3 was lower than those of groups 1 and 2. The amount of urine microalbumin and protein in group 1 were higher than those in groups 2 and 3. Also, more glomerulus basement membrane foot processes were preserved in groups 2 and 3. CONCLUSION: In conclusion, CSVA has beneficial preventive tendencies towards diabetic nephropathy in both in vitro and in vivo models.
