Arctiin inhibits adipogenesis in 3T3-L1 cells and decreases adiposity and body weight in mice fed a high-fat diet.
- Author:
Byulchorong MIN
1
;
Heejin LEE
;
Ji Hye SONG
;
Myung Joo HAN
;
Jayong CHUNG
Author Information
- Publication Type:Original Article
- Keywords: Arctiin; adipogenesis; AMP kinase; 3T3-L1 cells; high-fat diet
- MeSH: 3T3-L1 Cells*; Adenylate Kinase; Adipocytes; Adipogenesis*; Adipose Tissue; Adiposity*; AMP-Activated Protein Kinases; Animals; Blotting, Western; Body Weight*; Diet; Diet, High-Fat*; Intra-Abdominal Fat; Lipoprotein Lipase; Mice*; Mice, Obese; Obesity; Phosphorylation; PPAR gamma; Sterol Regulatory Element Binding Protein 1; Triglycerides; Weights and Measures
- From:Nutrition Research and Practice 2014;8(6):655-661
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/OBJECTIVES: The purpose of this study was to examine the effects and associated mechanisms of arctiin, a lignan compound found in burdock, on adipogenesis in 3T3-L1 cells. Also, the effects of arctiin supplementation in obese mice fed a high-fat diet on adiposity were examined. MATERIALS/METHODS: 3T3-L1 cells were treated with arctiin (12.5 to 100 microM) during differentiation for 8 days. The accumulation of lipid droplets was determined by Oil Red O staining and intracellular triglyceride contents. The expressions of genes related to adipogenesis were measured by real-time RT-PCR and Western blot analyses. For in vivo study, C57BL/6J mice were first fed either a control diet (CON) or high-fat diet (HF) to induce obesity, and then fed CON, HF, or HF with 500 mg/kg BW arctiin (HF + AC) for four weeks. RESULTS: Arctiin treatment to 3T3-L1 pre-adipocytes markedly decreased adipogenesis in a dose-dependent manner. The arctiin treatment significantly decreased the protein levels of the key adipogenic regulators PPARgamma and C/EBPalpha, and also significantly inhibited the expression of SREBP-1c, fatty acid synthase, fatty acid-binding protein and lipoprotein lipase. Also, arctiin greatly increased the phosphorylation of AMP-activated protein kinase (AMPK) and its downstream target phosphorylated-acetyl CoA carboxylase. Furthermore, administration of arctiin significantly decreased the body weight in obese mice fed with the high-fat diet. The epididymal, perirenal or total visceral adipose tissue weights of mice were all significantly lower in the HF + AC than in the HF. Arctiin administration also decreased the sizes of lipid droplets in the epididymal adipose tissue. CONCLUSIONS: Arctiin inhibited adipogenesis in 3T3-L1 adipocytes through the inhibition of PPARgamma and C/EBPalpha and the activation of AMPK signaling pathways. These findings suggest that arctiin has a potential benefit in preventing obesity.
