Analysis of flavonoids in Coreopsis tinctoria by integrating 2D-TLC and HPLC-IT-TOF-MS.
10.19540/j.cnki.cjcmm.20190118.002
- Author:
Mi-Mi REN
1
;
Ying XIA
2
;
Zi-Wei FENG
1
;
Bing-Yu ZHUO
1
;
Sheng-Li WEI
1
;
Yuan ZHANG
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs of Ministry of Education Beijing 102488, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
2D-TLC;
Coreopsis tinctoria;
HPLC-IT-TOF-MS;
flavonoids
- MeSH:
Chalcones;
analysis;
Chromatography, High Pressure Liquid;
Coreopsis;
chemistry;
Flavonoids;
analysis;
Phytochemicals;
analysis;
Plant Extracts;
analysis;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2019;44(7):1403-1409
- CountryChina
- Language:Chinese
-
Abstract:
The qualitative analysis of flavonoids in Coreopsis tinctoria was carried out by a combination of 2 D-TLC and HPLC-IT-TOF-MS. The separation was conducted on 2 D-TLC and a Phenomenex Kinetex Evo C_(18) column(2.1 mm×100 mm, 2.6 μm) with methanol-0.05% aqueous formic acid by gradient elution. Electrospray ionization-(ESI) source was applied and operated in both positive and negative ionization modes. Eighteen flavonoids including three flavonoids, one flavonol, nine flavonones, one flavanonol and four chalcones, were putatively identified from the flavone-enriched fraction of C. tinctoria. 2 D-TLC could separate the flavonoids from C. tinctoria. HPLC-IT-TOF-MS was able to quickly and accurately analyze the flavonoids in C. tinctoria. The results would provide experimental information for the efficacy material basis clarification of C. tinctoria.
