Spectrum-effect relationships on enriching blood activities of aerial parts of Angelica sinenis.
10.19540/j.cnki.cjcmm.20190118.007
- Author:
Jie-Li LIU
1
;
Xia MA
2
;
Ying ZHANG
2
;
Dong-Mei XING
2
;
Shu-Fang DENG
2
;
Yu-Jing SUN
1
;
Ya-Li WANG
3
Author Information
1. Gansu University of Chinese Medicine Lanzhou 730000, China Key Laboratory of Quality and Standard of Traditional Chinese Medicine in Gansu Province Lanzhou 730000, China.
2. Gansu University of Chinese Medicine Lanzhou 730000, China.
3. Gansu University of Chinese Medicine Lanzhou 730000, China Key Laboratory of Quality and Standard of Traditional Chinese Medicine in Gansu Province Lanzhou 730000, China the Research Insitute of Industrial Development of Angelica sinesis in Gansu Province Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
chromatography fingerprint;
enriching blood activities;
partial least squares regression;
spectrum-effect relationship;
the aerial parts of Angelica sinenis
- MeSH:
Angelica;
chemistry;
Animals;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
pharmacology;
Mass Spectrometry;
Medicine, Chinese Traditional;
Mice;
Plant Components, Aerial;
chemistry
- From:
China Journal of Chinese Materia Medica
2019;44(7):1416-1424
- CountryChina
- Language:Chinese
-
Abstract:
Ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to establish the chromatography fingerprint for aerial parts of Angelica sinenis(AAS) from 10 different places. Acetyl-phenyl-hydrazine(APH) was used to duplicate the mouse model of blood deficiency. Then partial least squares regression was used to investigate the spectrum-effect relationship between the relative contents and the data of enriching blood pharmacodynamics efficacy. The results showed that the three groups of high, medium and low doses of AAS had certain enriching blood activities(P<0.05), and the high dose group had the best effect(P<0.01). The contribution degree of the AAS to enriching blood activities of each common peaks were determined by PLS regression coefficient. Among them, 7 common peaks, including P17(unknown), P18(unknown), P19(unknown), P28(alisol B 23-acetate or its isomer), N5(luteolin), N11(1-caffeoylquinicacid,1-O-caffeoylquinic acid) and N14(unknown), contributed significantly to the effect of enriching blood activities. This paper dealed with the investigation on the spectrum-effect relationship between enriching blood activities and LC-MS chromatography fingerprint of AAS, and determination of the effective compositions of AAS with enriching blood activities. It provided theoretical foundation for the comprehensive development and utilization of AAS.
