Mechanisms of tanshinone Ⅱ_A in reducing 4-HNE-induced hepatocyte damage by activating PPARα.
10.19540/j.cnki.cjcmm.20190305.002
- Author:
Qian-Yu QIAN
1
;
Na YING
1
;
Zhen YANG
1
;
Li ZHOU
1
;
Qing-Sheng LIU
2
;
Zi-Yi HU
1
;
Chun-Lei FAN
1
;
Song-Tao LI
1
;
Xiao-Bing DOU
1
Author Information
1. College of Life Science,Zhejiang Chinese Medical University Hangzhou 310053,China.
2. Department of Geratology,Hangzhou Hospital of Traditional Chinese Medicine Hangzhou 310007,China.
- Publication Type:Journal Article
- Keywords:
4-HNE;
PPARα;
PPRE;
tanshinone ⅡA
- MeSH:
Aldehyde Oxidoreductases;
metabolism;
Aldehydes;
Animals;
Cell Line;
Diterpenes, Abietane;
pharmacology;
Hepatocytes;
drug effects;
Lipid Peroxidation;
Mice;
Oxidative Stress;
PPAR alpha;
metabolism
- From:
China Journal of Chinese Materia Medica
2019;44(9):1862-1868
- CountryChina
- Language:Chinese
-
Abstract:
Tanshinone Ⅱ_A( Tan Ⅱ_A),the liposoluble constituents of Salvia miltiorrhiza,can not only ameliorate the lipidic metabolism and decrease the concentration of lipid peroxidation,but also resist oxidation damage,scavenge free radicals and control inflammation,with a protective effect on prognosis after liver function impairment. Therefore,the studies on the exact mechanism of Tan Ⅱ_A in protecting the liver can provide important theoretical and experimental basis for the prevention and treatment effect of Tan Ⅱ_A for liver injury. In the present study,the protective effects and mechanism of Tan Ⅱ_A on 4-hydroxynonenal( 4-HNE)-induced liver injury were investigated in vitro. Normal liver tissues NCTC 1469 cells were used to induce hepatocytes oxidative damages by 4-HNE treatment. The protective effect of Tan Ⅱ_A on hepatocytes oxidative damages was detected by release amount of lactate dehydrogenase( LDH) analysis and hoechst staining. The protein expression changes of peroxisome proliferator-activated receptor α( PPARα) and peroxisome proliferator response element( PPRE) were analyzed by Western blot analysis in NCTC 1469 cells before and after Tan Ⅱ_A treatment. The gene expression changes of fatty aldehyde dehydrogenase( FALDH) were analyzed by Real-time polymerase chain reaction( PCR) analysis. The results showed that 4-HNE increased the release amount of LDH,lowered the cell viability of NCTC 1469 cells,and Tan Ⅱ_A reversed 4-HNE-induced hepatocyte damage. Western blot analysis and RT-PCR analysis results showed that 4-HNE decreased the expression of PPARα and FALDH and increased the expression of 4-HNE. However,the expression of PPARα and FALDH were increased significantly and the expression of 4-HNE was decreased obviously after Tan Ⅱ_A treatment. This study confirmed that the curative effect of Tan Ⅱ_A was obvious on hepatocytes damage,and the mechanism may be associated with activating PPARα and FALDH expression as well as scavenging 4-HNE.
