Development and application of in-situ microscopy in on-line monitoring of cell biomass.

Author: Yuanshan WANG ¹ ; Wenhui HAO ¹ ; Zheming WU ¹ ; Kun NIU ¹ ; Meihua GONG ¹
Author Information

1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Publication Type:Journal Article
Keywords: biomass; fermentation process; in-situ microscope (ISM); on-line monitor
MeSH: Biomass; Bioreactors; Biotechnology; Fermentation; Microscopy
From:Chinese Journal of Biotechnology 2019;35(9):1607-1618
CountryChina
Language:Chinese
Abstract: With the rapid development of modern biotechnology, fermentation process is increasingly important in industrial production. To guarantee the stability of products, fermentation process should be elaborately monitored and controlled. Biomass is an important parameter for on-line monitoring in bioprocesses because biomass can reflect cell growth in a bioreactor directly. In-situ microscope, a non-invasive and image-analysis based technology, can real-time monitor cells in biological process. This review summarizes the development and application of in-situ microscopy in biomass monitoring.