Micro-CT evaluation of a novel periodontal ligament simulation technique for dental experimental models
- Author:
Fawzia AlZahrani
1
Author Information
1. Adelaide Dental School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia, 5005 Australia.
- Collective Name:Lindsay Richards
- Publication Type:Journal Article
- Keywords:
Experimental model;
micro-CT;
periodontal ligament;
rubber;
simulation
- From:Archives of Orofacial Sciences
2018;13(2):93-103
- CountryMalaysia
- Language:English
-
Abstract:
A reliable technique for simulation of the periodontal ligament (PDL) is required for in vitro studies. This
paper proposes a simple technique to construct a tooth-PDL-bone experimental model with a reproducible PDL
layer of a uniform width. In a preliminary study, two PDL simulation techniques were compared; transitional wax
technique: wax layer was used to create a space for the PDL simulating material (light-body silicon) and direct
rubber application technique: PDL simulating material (rubber die-spacer) was painted directly on the root surface.
In both techniques, teeth were mounted in acrylic resin to simulate the supporting bone. The tooth-PDL-bone
models were scanned using SkyScan 1076 micro-CT scanner and PDL-layer width was measured at selected sites
on the roots using CTAn software. Based on the results of the preliminary study, 10 experimental models were
constructed using the direct rubber application technique to confirm the reproducibility and consistency of the PDL
layer width using micro-CT. Intra-class correlation coefficients (ICC) were calculated to assess the reproducibility.
The transitional wax technique showed significantly greater variability in the PDL layer width when compared with
the direct rubber application technique (F=66.0, p<0.001). The direct rubber application technique showed excellent
reproducibility (ICC= 0.94; 95% confidence interval: 0.86, 0.98).
- Full text:1.2018my0160.pdf
