Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas.
- Author:
Hyung Seo PARK
1
;
Se Hoon KIM
;
Hyoung Jin PARK
;
Mee Young LEE
;
Young Hee HAN
Author Information
1. Department of Physiology, College of Medicine, Konyang University, Nonsan, Chungnam-do, Korea. hspark@konyang.ac.kr
- Publication Type:Original Article
- Keywords:
Pancreatic secretion;
Progesterone;
Estradiol-17beta;
Secretin;
CCK;
Acetylcholine
- MeSH:
Acetylcholine;
Amylases;
Animals;
Cholecystokinin;
Estradiol;
Gonads*;
Infusions, Intra-Arterial;
Masks;
Pancreas*;
Progesterone;
Rats*;
Secretin;
Steroids*
- From:The Korean Journal of Physiology and Pharmacology
2003;7(4):217-222
- CountryRepublic of Korea
- Language:English
-
Abstract:
To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-17beta on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-17beta did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin, cholecystokinin (CCK), and acetylcholine markedly stimulated pancreatic fluid and amylase secretion. Progesterone initially enhanced secretin-induced amylase secretion, but this stimulatory response declined thereafter to basal value. Moreover, secretin-induced fluid secretion was not affected by infusion of progesterone. Therefore, initial increase of secretion-induced amylase secretion by progesterone seems to be a non-specific action by washout effect of secretin. Estradiol-17beta failed to change the secretin-induced fluid and amylase secretion. Both progesterone and estradiol-17beta did not exert any influence on CCK-induced fluid and amylase secretion. Acetylcholine-induced exocrine secretion of isolated perfused pancreas also was not affected by intra-arterial infusion of progesterone or estradiol-17beta. It is concluded from the above results that progesterone could enhance the spontaneous pancreatic fluid and amylase secretion of isolated perfused rat pancreas through non-genomic short- term action, and that these effects could be masked by more potent stimulants such as secretin, CCK, and acetylcholine.
