VEGF-C Promotes Cervical Cancer Invasion via Regulation of microRNA-145/Smad Interacting Protein 1
- VernacularTitle:VEGF-C调节miR-145/SIP1增强宫颈癌细胞侵袭能力
- Author:
Yang CHENG
1
;
Zheng-Tian GU
Author Information
1. 广州市第一人民医院妇产科
- Keywords:
cervical cancer;
vascular endothelial growth factor C;
microRNA-145;
Smad interacting protein 1;
invasion
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2018;39(2):215-219
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the role of microRNA-145/Smad interacting protein 1(SIP 1)in VEGF-C-enhanced cervical cancer metastasis.[Methods]Cervical cancer cell line SiHa cells were cultured and treated with VEGF-C to observe its effect on the expression of miR-145 and SIP1. After transfection with specific SIP1 siRNA,the invasion number of cultured cells were calculated by transwell chamber assay.[Results]Treatment with VEGF-C(100 ng/mL)for 12 h,24 h and 48 h all reduced miR-145 expression,with the expression abundance of(82.4±6.4)% (P<0.05),(72.5±7.2)%(P<0.01),and(60.6±9.6)%(P<0.001),respectively,when compared to control.Meanwhile, the same treatment with VEGF-C also increased SIP1 protein expression,with the expression abundance of(142.4 ± 16.5)%(P<0.05),(183.6 ± 11.4)%(P<0.01)and(220.8 ± 15.7)%(P<0.001),respectively. The transfection of miR-145 mimic significantly impaired VEGF-C effect on SIP1 expression. Finally,VEGF-C promoted SiHa cell invasion,which was largely inhibited by the tranfection of SIP siRNA with the inhibitory rate of(56.6±10.3)%(P<0.01).[Conclusion]VEGF-C downregulates miR-145,thus increases SIP1 expression and promotes cervical cancer cell invasion,which may contributes to cervical cancer malignant progression.
