The temporal profile of astrocytes and Jak-STAT signal pathway after spinal ischemia and reperfusion injury in rabbits
10.3760/cma.j.issn.1001-4497.2018.02.011
- VernacularTitle:星形胶质细胞与JAK-STAT通路在兔脊髓缺血再灌注损伤后变化规律的研究
- Author:
Yunlu WANG
1
;
Lei TIAN
;
Shiyao LIU
;
Zhigao MA
;
Siyu HOU
;
Yanwei YANG
;
Huixian LI
;
Mu JIN
;
Xiuhua DONG
;
Jiakai LU
;
Weiping CHENG
Author Information
1. 100029,首都医科大学附属北京安贞医院 北京市心肺血管疾病研究所麻醉科
- Keywords:
Spinal cord;
Injury,ischemia-reperfusion;
Astrocytes;
GDNF;
BDNF;
Jak-STAT signaling pathway
- From:Chinese Journal of Thoracic and Cardiovascular Surgery
2018;34(2):104-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the evolution of astrocytes,GDNF,BDNF and Jak-STAT signal pathway after spinal cord ischemia-reperfusion injury in rabbits.Methods Spinal cord ischemia was induced by means of balloon occlusion of the infrarenal aorta for 22 minutes in 54 male New Zealand white rabbits.We assigned rabbits to 9 groups (n =6),one sham group,eight operation groups.The operation process in the sham group was the same as the operation group except the ischemia reperfusion of the spinal cord.At 0 h,1 h,2 h,3 h,8 h,24 h,48 h and 72 h after reperfusion,animals were sarcrificed and the spinal cord was removed for histologic,immunohistochemical study and western blotting.Results Normal neurons were decreased with the extension of reperfusion time.Levels of GFAP increased at 3 h and reached a peak at 48 h after reperfusion.GDNF was increased reaching two peaks after injury,the first peak was at 3 h,the second was at 72 h.BDNF level was increased and peaked at 24 h after reperfusion.The expression of p-STAT3 showed a biphasic pattern which peaked at 1h and 48 h.GFAP,GDNF,BDNF were rare and the level of p-STAT3 could be neglected in sham group.Conclusion Spinal cord ischemia-reperfusion injury could induce the activation of astrocytes,the expression of GDNF,BDNF and the activation of JakSTAT signal pathway.They showed different expression rules in this study.
