Simultaneous determination of six constituents in Tuiyin Mixture by HPLC
10.3969/j.issn.1001-1528.2018.05.017
- VernacularTitle:HPLC法同时测定退银合剂中6种成分
- Author:
Jing LU
1
;
Xiao-Jing NIU
;
Xiao-Ying DUAN
Author Information
1. 河南中医药大学第一附属医院
- Keywords:
Tuiyin Mixture;
chlorogenic acid;
loganin;
paeoniflorin;
baicalin;
salvianolic acid B;
paeonol;
HPLC
- From:
Chinese Traditional Patent Medicine
2018;40(5):1093-1096
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,loganin,paeoniflorin,baicalin,salvianolic acid B and paeonol in Tuiyin Mixture (Moutan Cortex,Paeoniae Radix Rubra,Lonicerae japonicae Caulis,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic ZORBAX SB-Aq column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 235 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r≥0.999 7),whose average recoveries were 97.1％-100.6％ with the RSDs of 1.35％-2.28％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Tuiyin Mixture.
