Chemical constituents from Pogonatherum crinitum and their in vitro anti-HBV activities
10.3969/j.issn.1001-1528.2018.02.023
- VernacularTitle:金丝草化学成分及其体外抗HBV活性
- Author:
Xiao-Xu YUAN
1
;
Hong-Bo LI
;
Zhi-Feng YIN
;
Hui-Na WANG
;
Gui-Qin ZHAO
Author Information
1. 承德医学院中药研究所
- Keywords:
Pogonatherum crinitum (Thunb.) Kunth;
chemical constituents;
isolation and identification;
in vitro anti-HBV activities
- From:
Chinese Traditional Patent Medicine
2018;40(2):363-368
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Pogonatherum crinitum (Thunb.) Kunthand and their in vitro anti-HBV activities.METHODS The ethyl acetate and n-butanol fractions of 70％ ethanol extract from P.crinitum were isolated and purified by AB-8 macroporous resin,silica column and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-HBV activities of the isolated compounds were tested in the model of HepG2.2.15 cells.RESULTS Eight compounds were isolated and identified as 6,8,4'-trihydroxy-7,3'-dimethoxyisoflavone (1),quercetin-7,4'-dimethylester-5-O-β-D-glucopyranoside (2),8-(1-(3,4-dihydroxyphenyl) ethyl) quercetin (3),chrysoeiml-7-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside (4),kaempferol-3-O-(2",3"-di-O-E-p-cou-maroyl)-α-L-rhamnopyranoside (5),1,3,7-trihydroxyxanthone-2-C-β-D-glucopyra-noside (6),luteolin-6-C-β-D-boivinopyranoside (7),luteolin-6-C-β-D-glucopyranoside (8).Compounds 3,5,7,8 had certain inhibitory effects on HBsAg with the IC50 values of 96.6,127.1,72.4 and 54.5 μg/mL.Compounds 7,8 had certain inhibitory effects on HBeAg with the IC50 values of 81.0,61.3 μg/mL.CONCLUSION Compounds 1-6 are isolated from genus Pogonatherum for the first time,compounds 3、5、7、8 have certain in vitro anti-HBV activities.
