Simultaneous determination of eleven amino acids in Gynostemma pentaphyllum by UFLC-QTRAP-MS/MS

VernacularTitle:UFLC-QTRAP-MS/MS法同时测定绞股蓝中11种氨基酸
Author: Yun MA ¹ ; Jing CAI ; Yuan-Jiao ZHANG ; Le-Jun JI ; Jian-Wei CHEN ; Xiang LI
Author Information

1. 南京中医药大学药学院
Keywords: Gynostemma pentaphyllum (Thunb.) Makino; underivatized amino acids; UFLC-QTRAP-MS/MS
From: Chinese Traditional Patent Medicine 2018;40(1):133-137
CountryChina
Language:Chinese
Abstract: AIM To establish a UFLC-QTRAP-MS/MS method for the simultaneous content determination of phenylalanine,isoleucine,methionine,valine,proline,tyrosine,aspartic acid,histidine,arginine,lysine and glutamic acid in Gynostemma pentaphyllum (Thunb.) Makino.METHODS The analysis of aqueous extract of G.pentaphyllum was performed on a Waters XBridge Amide column (2.1 mm × 100 mm,3.5 μm),with the mobile phase comprising of water-acetonitrile (containing 0.2％ formic acid) flowing at 0.6 mL/min in a gradient elution manner.RESULTS Eleven amino acids showed good linear relationships within their own ranges (r ＞0.998 9),whose average recoveries were 90.74％-103.05％ with the RSDs of 0.61％-5.00％.CONCLU-SION This accurate,stable and reproducible method can be used for the quality control of G.pentaphyllum.