Effect of dexmedetomidine pretreatment on contents of glutamic acid and γ-aminobutyric acid dur-ing focal cerebral ischemia-reperfusion in rats
10.3760∕cma.j.issn.0254-1416.2018.07.029
- VernacularTitle:右美托咪定预先给药对大鼠局灶性脑缺血再灌注时谷氨酸和γ-氨基丁酸含量的影响
- Author:
Sheng LIN
1
;
Guangli ZHOU
;
Zhijian FU
Author Information
1. 264001,烟台市烟台山医院麻醉科
- Keywords:
Dexmedetomidine;
Brain;
Reperfusion injury;
Glutamic acid;
gamma-Ami-nobutyric acid
- From:Chinese Journal of Anesthesiology
2018;38(7):886-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of dexmedetomidine pretreatment on contents of glu-tamic acid and γ-aminobutyric acid during focal cerebral ischemia-reperfusion ( I∕R) in rats. Methods Thirty-six clean-grade healthy male Wistar rats, aged 10 months, weighing 250-300 g, were divided into 3 groups using a random number table method: sham operation group (group S), I∕R group, and dexme-detomidine group (group D). Focal cerebral I∕R was induced by occlusion of the left middle cerebral artery for 30 min followed by reperfusion in anesthetized rats. The left middle cerebral artery was only isolated but not occluded in group S. Sterile normal saline 1 ml was intraperitoneally injected, and 30 min later the model of focal cerebral I∕R was established in group I∕R. Rats were sacrificed at the end of reperfusion, and brains were removed for determination of contents of glutamic acid and γ-aminobutyric acid in brain tis-sues and for examination of ultrastructure (with an electron microscope). Results Compared with group S, the content of glutamic acid was significantly increased, and the content of γ-aminobutyric acid was de-creased in I∕R and D groups (P＜0. 05). Compared with group I∕R, the content of glutamic acid was signif-icantly decreased, and the content of γ-aminobutyric acid was increased in group D (P＜0. 05). The dam-age to the ultrastructure of brain tissues was significantly attenuated in group D when compared with group I∕R. Conclusion Dexmedetomidine pretreatment can reduce focal cerebral I∕R injury through decreasing the content of glutamic acid and increasing the content of γ-aminobutyric acid in rats.
