Effect of capsaicin on cardiac dysfunction in diabetic rats: an in vitro experiment
10.3760∕cma.j.issn.0254-1416.2017.11.009
- VernacularTitle:辣椒素对糖尿病大鼠心脏功能障碍的影响:离体实验
- Author:
Ying MENG
1
;
Tuping LI
;
Zheng GUO
Author Information
1. 山西医科大学麻醉学系
- Keywords:
Capsaicin;
Diabetes mellitus;
Heart function tests
- From:Chinese Journal of Anesthesiology
2017;37(11):1314-1317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of capsaicin on cardiac dysfunction in diabetic rats u-sing an in vitro experiment. Methods Healthy male Sprague-Dawley rats, weighing 240-260 g, in which type 1 diabetes mellitus was induced by intraperitoneal streptozotocin 50 mg∕kg, were studied. Eighteen di-abetic rats were selected at 8 weeks after successful establishment of the model and divided into 3 groups (n=6 each)using a random number table: diabetes mellitus group(DM group), capsaicin group(CAP group)and capsaicin plus capsazepine group(CPZ group). Another 8 rats with normal blood glucose served as control group(C group). Rat hearts were quickly removed under deep anesthesia and retrogradely perfused with an oxygen-saturated K-H solution(at 37℃)using a Langendorff apparatus. Cardiac function was maintained stable for 10 min. The hearts were continuously perfused with K-H solution for 30 min in C and DM groups. The hearts were perfused with K-H solution for 20 min, and capsaicin(1.4×10-9g∕L)was then infused for 10 min via the branch of aortic cannula using micro pump in CAP group. The hearts were perfused with K-H solution for 10 min, and capsaicin receptor-transient receptor potential vanilloid type 1 antagonist capsazepine(1.4×10-7g∕L)was then infused for 10 min followed by infusion of capsaicin(1.4× 10-9g∕L)at 0.5 ml∕min for 10 min via the branch of aortic cannula using a micro-pump in CPZ group. Left ventricular systolic pressure(LVSP), left ventricular developed pressure(LVDP), left ventricular end-di-astolic pressure(LVEDP), heart rate, the maximum rate of increase or decrease in left ventricular pres-sure(±dp∕dtmax)were recorded at 10, 20 and 30 min of continuous infusion(T1-3). Results There was no significant difference in LVEDP and ± dp∕dtmaxat each time point among the four groups(P>0.05). Compared with C group, LVSP and LVDP were significantly decreased at T1-3in DM and CPZ groups and at T1-2in CAP group, and heart rate was significantly decreased at T1-3in DM, CPZ and CAP groups(P<0.05). Compared with DM group, LVSP and LVDP were significantly increased at T3in CAP group(P<0.05), and no significant change was found in LVSP or LVDP in CPZ group(P>0.05). LVSP and LVDP were significantly lower at T3in CPZ group than in CAP group(P<0.05). Conclusion Capsaicin can mitigate cardiac dysfunction in diabetic rats, and the mechanism is related to activating transient receptor potential vanilloid type 1.
