Content Determination of Total Flavones and Polysaccharides in Hedysari Radix in Different Producing Areas in Gansu Province
10.3969/j.issn.1005-5304.2018.02.018
- VernacularTitle:甘肃不同产地红芪中总黄酮及总多糖含量测定研究
- Author:
Xiu-Juan YANG
1
;
Zhi-Jun YANG
;
Peng-Xian NIU
;
Zi-Yu PAN
;
Jing SHAO
;
Shuo LI
Author Information
1. 甘肃中医药大学
- Keywords:
Hedysari Radix;
ultraviolet spectroscopy;
calycosin-7-glucoside;
glucose;
total flavones;
total polysaccharides
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(2):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for content determination of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province.Methods The contents of total flavones and polysaccharides in Hedysari Radix in different producing areas in Gansu Province were determined by ultraviolet spectroscopy with calycosin-7-glucoside and glucose as reference substance, and the wavelength was set at 260 nm and 484 nm.Results The contents were from 2.82 mg/g to 6.79 mg/g for total flavones and from 106.14 mg/g to 746.40 mg/g for total polysaccharides in Hedysari Radix in different producing areas in Gansu Province. The recoveries of total flavones and total polysaccharides were 97.96％ and 102.90％, respectively.Conclusion There was difference in contents of total flavones and polysaccharides of Hedysari Radix in different producing areas in Gansu Province, and the method of using ultraviolet spectroscopy is simple, reproducible, accurate and reliable, which can be preferably used as the method for content determination of total flavones and polysaccharides in Hedysari Radix.
