Uptake Study on Apigenin in Caco-2 Cells
10.3969/j.issn.1005-5304.2018.02.017
- VernacularTitle:芹菜素在Caco-2细胞的摄取研究
- Author:
Shan GUO
1
;
Chun-Hong TIAN
;
Yuan HE
;
Xiao-Xia ZHANG
;
Li TIAN
Author Information
1. 新疆医科大学中医学院
- Keywords:
apigenin;
Caco-2 cells;
uptake;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(2):74-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the uptake property of apigenin in Caco-2 cells.Methods Caco-2 cell monolayer model was used to research the effects of apigenin on cell uptake in different times, concentrations, temperatures, pH values, and P-gp inhibitors. HPLC was used to determine the content of apigenin in cells.Results The linearity of apigenin concentration curve was found in good linear range of 0.33–80 μg/mL (r=0.9994). Uptake determination of apigenin in Caco-2 cells depended a certain of time, concentration and pH values, and P-gp inhibitors could not increase the apigenin uptake.Conclusion This method is simple and sensitive. P-gp is not involved in apigenin transport process, and uptake of apigenin in Caco-2 cells is mainly passive transported.
