Study on the Extraction Process of Herbal Pair Puerarin-Berberine by Plackett-Burman Design and Box-Behnken Response Surface Methodology
- VernacularTitle:基于PB设计和BBD响应面法优化葛根-黄连药对提取工艺
- Author:
Cheng CHEN
1
;
Guoping LUO
;
Suomin FENG
;
Cunlao ZHANG
;
Mengru YAN
;
Yanru GUO
Author Information
1. 西安医学院药学院 西安710021
- Keywords:
Puerarin-berberine;
Extraction process;
Plackett-Burman design;
Box-Behnken response surface methodology
- From:
China Pharmacist
2018;21(2):228-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the alcohol extraction process of herbal pair puerarin-berberine.Methods:Based on the etha-nol reflux extraction,the extraction quantity of total flavonoids,total alkaloid,puerarin and berberine hydrochloride were used as the e-valuation index,and the independent variables included the drug particle,ratio of solid to liquid, ethanol concentration, reflux dura-tion and reflux times. Significance analysis was evaluated by Plackett-Burman design,and then the extraction process was optimized by Box-Behnken response surface methodology.Results:The optimal extraction conditions of drug pair puerarin-berberine were as follows:the drug particle was 80 mesh,the ratio of solid to liquid was 1:13,the ethanol concentration was 75%,the reflux time was 60 min, and the reflux times was 4. Under the above conditions, the extraction quantity of total flavonoids, alkaloid, puerarin and berberine hydrochloride was 120.34,56.99,109.63 and 39.26 mg ·ml-1, respectively.Conclusion: The extraction process of herbal pair puerarin-berberine is reasonable and feasible optimized by Plackett-Burman design and Box-Behnken response surface methodology.
