Inhibitory growth effect of honokiol and BMP7 in human colon cancer cells
10.3969/j.issn.1001-1978.2018.07.023
- VernacularTitle:和厚朴酚抑制结肠癌细胞增殖与骨形态发生蛋白7关系的研究
- Author:
Rong-Xing LIU
1
,
2
;
Pei HU
;
Yan MA
;
Wen-Yan REN
;
Lin-Yun ZHOU
;
Bai-Cheng HE
;
Wen-Juan SUN
Author Information
1. 重庆市生物化学与分子药理学重点实验室
2. 重庆医科大学药理学教研室
- Keywords:
honokiol;
colon cancer;
HCT116 cells;
anti-proliferation;
apoptosis;
BMP7
- From:
Chinese Pharmacological Bulletin
2018;34(7):1012-1019
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the anti-proliferation effect of honokial on human colon cancer cells HCT116 and the possible mechanism. Methods Crystal stai-ning and flow cytometry assay were introduced to test the proliferation inhibitory and cell cycle arrested effect of honokial on HCT116 cells. Western blot was used to analyse the expression of PCNA and induction effect of apoptosis. Western blot and PCR assay were conducted to assess the change of BMP7. Via exogenous adenovi-ruses of BMP7 and its antibody combined with honoki-al,crystal violet staining and Western blot were used to analyse the effect on HCT116 cells. Results Honoki-al inhibited the growth of HCT116 in a time-and con- centration-dependent way,induced apoptosis and arres-ted at G2 phase; Western blot assay showed honokial up-regulated the level of PCNA and BMP7,and down-regulated the expression of Bcl-2; Western blot result also showed that exogenous adenoviruses of BMP7 en-hanced the effect of honokial on proliferation inhibition and apoptosis, while BMP7 antibody reversed such effects of honokial. Conclusion Honokial can inhibit the proliferation and induce apoptosis on HCT116 cells,which may be mediated by the up-regulation of BMP7.
