Effects of icariin on acute lung injury in mice induced by activation of the complement alternative pathway
10.3969/j.issn.1001-1978.2018.07.019
- VernacularTitle:淫羊藿苷对补体旁路激活诱导的小鼠急性肺损伤的保护作用
- Author:
Jing GUO
1
;
Jiao LI
;
Lin ZHANG
;
Yi YANG
;
Fang-Juan ZHU
;
Min LI
;
Qian-Yun SUN
Author Information
1. 贵州医科大学省部共建药用植物功效与利用国家重点实验室
- Keywords:
icariin;
complement alternative pathway;
acute lung injury;
cobra venom factor;
NF-κB p65;
inflammation
- From:
Chinese Pharmacological Bulletin
2018;34(7):988-993
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effect and mechanism of icariin ( ICA ) on acute lung injury ( ALI) in mice induced by activation of the comple-ment alternative pathway. Methods 32 healthy KM mice were randomly divided into four groups: the nor-mal group, the model group, the PDTC group and the Icariin group, which received 7-day intragastric admin-istration respectively. Then cobra venom factor ( CVF) was used to activate specifically complement alternative pathway to induce acute lung injury in mice by intrave-nous injection. Myeloperoxidase ( MPO ) activity of lung homogenate, the cell count and the protein con- tent of bronchoalveolar lavage fluid ( BALF ) were measured. The concentration of IL-6, TNF-α, P-selec-tin and ICAM-1 in BALF and serum were determined by ELISA. The pathological change of lung tissue was observed by HE staining. The phosphorylation of NF-κB p65 in lung tissues was checked by immunohisto-chemistry. The effect of the transcriptional activity of NF-κB signal pathway in microvascular endothelial cells was measured by employing dual-luciferase re-porter assay system. Results ICA reduced MPO ac-tivity of lung homogenate, the cell count and the con-tent of IL-6, TNF-α, P-selectin in BALF obviously. The level of TNF-α, P-selectin and ICAM-1 in serum was decreased, the pulmonary inflammatory cell infil-tration was reduced, the phosphorylation of NF-κB p65 in lung was inhibited significantly and the transcrip-tional activity of NF-κB was also down-regulated. Con-clusion ICA can alleviate acute inflammatory re-sponse of ALI mice induced by activation of the com-plement alternative pathway. The mechanism may be highly related to the inhibition of inflammatory cell in-filtration in lung tissue, the down-regulation of phos-phorylation of NF-κB p65 and nuclear transcriptional activity.
