Sal B attenuates rat hepatocytes injury induced by hypoxia/reoxygenation via modulation of SIRT1/NF-κB/p53 pathway
10.3969/j.issn.1001-1978.2018.05.018
- VernacularTitle:丹酚酸B通过调控SIRT1/NF-κB/p53通路减轻缺氧/复氧诱导的大鼠肝细胞损伤
- Author:
Lei WAN
1
;
Qing-Song CHEN
;
Zhuang ZHOU
;
Xiang-Yu ZHOU
;
Dao-Feng ZHENG
;
Zhong-Jun WU
Author Information
1. 重庆医科大学附属第一医院肝胆外科
- Keywords:
salvianolic acid B;
SIRT1;
NF-κB p65;
p53;
hypoxia/reoxygenation;
BRL-3A
- From:
Chinese Pharmacological Bulletin
2018;34(5):680-685
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of salvianolic acid B(Sal B) on the attenuation of rat hepatocyte in-jury induced by hypoxia/reoxygenation(H/R) and its possible molecular mechanism. Methods Rat hepato-cytes BRL-3A were cultured in vitro. H/R injury mod-el was established and then BRL-3A cells were pretrea-ted with Sal B. The viability of cells was measured by CCK-8 assay;the expression of ALT and AST was de-tected by microplate assay; the levels of TNF-α and IL-1β were determined by ELISA; the apoptosis was detected by flow cytometry;the protein and mRNA lev-els of SIRT1, NF-κB p65, p53, Bax and Bcl-2 were measured by Western blot and qPCR. Results H/R intervention decreased the viability and increased the apoptosis of cells;the production of ALT, AST, TNF-α and IL-1β was elevated;the protein and mRNA lev-els of SIRT1, Bcl-2 were reduced, but the levels of NF-κB p65, p53 and Bax increased. After pretreated with Sal B, the viability of cells increased while the apoptosis decreased; the expression of ALT, AST, TNF-α and IL-1β was inhibited;moreover,the protein and mRNA levels of SIRT1,Bcl-2 were enhanced,and the levels of NF-κB p65, p53 and Bax decreased sig-nificantly. Conclusion Sal B may attenuate rat hepa-tocyte injury induced by H/R via the SIRT1/NF-κB/p53 pathway.
