Protective effects of naringenin against hypoxic-ischemic brain damage in neonatal rats through suppression of NOD2/RIP2/NF-κB pathway
10.3969/j.issn.1001-1978.2018.03.023
- VernacularTitle:基于NOD2/RIP2/NF-κB信号通路研究柚皮素对新生大鼠缺血缺氧性脑损伤的保护作用
- Author:
Zhu-Ting FENG
1
;
Zhan-Hui FENG
;
Shi-Ping WANG
;
Yan-Ling JIN
;
Mei XIAO
;
Lan YE
Author Information
1. 贵州医科大学基础医学院药理学教研室 贵州贵阳 550025
- Keywords:
naringenin;
hypoxic-ischemic brain dam-age;
inflammation;
NOD2;
RIP2;
NF-κB
- From:
Chinese Pharmacological Bulletin
2018;34(3):418-422
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effects of naringin on hypoxic ischemic brain injury in neonatal rats and its mechanism. Methods Ninety-six healthy 7-day neonatal SD rats were randomly divided into sham operation group, hypoxic-ischemic brain damage group (HIBD group),HIBD with low-dose naringenin group(50 mg·kg-1, NG-L) and HIBD with high-dose naringenin group(100 mg·kg-1,NG-H). Neu-rological deficit, HE staining and brain water content were measured 48h after operation. Immunoblotting was used to detect the expressions of NOD2,RIP2 and NF-κB. Enzyme linked immunosorbent assay(ELISA) method was adopted to detect TNF-α and IL-1β ex-pression. Results Compared with HIBD group, the neurological deficit score decreased, the pathological damage was reduced, and the water content of brain tissues markedly decreased by naringenin(50,100 mg ·kg-1) treatment(P<0.05). Western blot revealed the down-regulation of NOD2,RIP2 and NF-κB by na-ringenin (50,100mg·kg-1) treatment (P<0.05). The content of TNF-α and IL-1β in brain tissues was lower than that of HIBD group (P <0.05). Conclu-sion Naringenin is likely to exert a protective role in neonatal rats of hypoxic ischemic brain injury perhaps through decreasing the expression of NOD2, RIP2 and NF-κB,and reducing the secretion of TNF-α and IL-1β.
