Catechin remits the inflammation in allergic asthma mice by suppressing NF-κB-TSLP signal pathway
10.3969/j.issn.1001-1978.2018.02.013
- VernacularTitle:儿茶素抑制NF-κB-TSLP通路缓解过敏性哮喘小鼠炎症反应
- Author:
Zeng-Feng PAN
1
;
Yuan ZHOU
;
Yan RUAN
;
Xia LUO
;
Ya-Jie YAN
;
Lian ZHOU
Author Information
1. 广州中医药大学中药学院
- Keywords:
catechin;
allergic asthma;
thymic stromal lymphopoietin;
Th2 inflammatory response;
anti-inflammation;
NF-κB
- From:
Chinese Pharmacological Bulletin
2018;34(2):207-212
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the mechanism of catechin in inhibiting Th2-driven inflammation responses of allergic asthma mice.Methods Female BALB/c mice were chosen to establish an allergic asthma model by sensitized and excited with OVA.Then,the administered group was intervened with catechin by series of dose (75,150,300 mg · kg-1).The proportion of peripheral leukocyte cells was analysed,and pathological changes in lung tissues were observed by HE dyeing.The levels of TSLP,IL-5,IL-13 in lung tissues and OVA specific IgE in mouse serum were measured using ELISA kit.NF-κB p65,IκB protein expression levels in lung tissues were investigated using Western blot,and NF-κB p65 nuclear translocation was detected with immunofluorescent technique.Results Catechin could not only effectively reduce the proportion of peripheral leukocyte cells,but relieve the levels of TSLP,Th2 inflammatory factor IL-5 and IL-13,and the activation of NF-κB signal pathway in lung tissues of allergic asthma mice.Conclusions Catechin can effectively relieve Th2 inflammation in allergic asthma mice induced by allergen OVA.The possible mechanism may be that it could reduce expression of TSLP by inhibiting the NF-κB signal.
