Simultaneous Determination of the Content of Berberine Hydrochloride,Baicalin and Osthole in Jinchan Zhiyang Capsules by HPLC
10.6039/j.issn.1001-0408.2018.12.09
- VernacularTitle:HPLC法同时测定金蝉止痒胶囊中盐酸小檗碱、黄芩苷和蛇床子素的含量
- Author:
Chuanjun HUANG
1
;
Yong MEI
;
Li YANG
;
Lei LUO
;
Bocheng ZENG
;
Tao LONG
;
Kaichao YUAN
;
Zhiwen QIAO
;
Xiaoxue CHEN
Author Information
1. 湖北省中医院药事部
- Keywords:
Jinchan zhiyang capsules;
HPLC;
Berberine hydrochloride;
Baicalin;
Osthole;
Content determination
- From:
China Pharmacy
2018;29(12):1621-1624
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for simultaneous determination of the content of berberine hydrochloride, baicalin and osthole in Jinchan zhiyang capsules. METHODS:HPLC method was adopted. The determination was performed in Hypersil BDS C18 column with mobile phase consisted of methanol-acetonitrile-0.1%phosphoric acid-three triethylamine(50∶30∶19∶1, V/V/V/V) at the flow rate of 1.0 mL/min. The detection wavelengths were set at 265 nm (berberine hydrochloride),280 nm (baicalin)and 322 nm(osthole). The column temperature was set at 30 ℃,and sample size was 10 μL. RESULTS:The linear range of berberine hydrochloride,baicalin and osthole were 80-800 ng(r＝0.999 8),60-600 ng(r＝0.999 9),60-600 ng(r＝0.999 6),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The limits of quantitation were 80,60,60 ng,respectively,and the limits of detection were 24,20,20 ng,respectively. The recovery rates were 97.4%-98.3%(RSD＝0.33%,n＝6),98.4%-99.6%(RSD＝0.42%,n＝6)and 96.9%-99.0%(RSD＝0.92%,n＝6),respectively. RSDs of durability tests were all lower than 1.2%. CONCLUSIONS:The method is simple, accurate, precise, stable, reproducible and durable. It can be used for simultaneous determination of berberine hydrochloride,baicalin and osthole in Jinchan zhiyang capsules.
