An association study on interaction of single nucleotide polymorphism of tryptophan hydroxylase 2 with negative life events on major depressive disorder
10.3760/cma.j.issn.1674-6554.2018.06.006
- VernacularTitle:色氨酸羟化酶2单核苷酸位点多态性和负性生活事件交互作用与抑郁症的关联研究
- Author:
Chunyang ZHANG
1
;
Xueyan ZHAO
;
Lin WANG
;
Xiuxian YANG
;
Xiaohui QIU
;
Jian ZHANG
;
Yanjie YANG
Author Information
1. 哈尔滨医科大学公共卫生学院医学心理学教研室
- Keywords:
Major depressive disorder;
Tryptophan hydroxylase 2;
Gene polymorphism;
Neg- ative life events;
Generalized multifactor dimensionality reduction
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(6):511-515
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the interaction between single nucleotide polymorphism of trypto-phan hydroxylase 2 (TPH2) (rs4570625,rs11178997,rs120074175) and negative life events and the asso-ciation with major depressive disorder (MDD) in a Chinese population.Methods Totally 300 cases of pa-tients with major depressive disorder and 300 healthy controls in northern China were enrolled and the ge-nomic DNA were extracted. PCR was used to detect the polymorphisms of rs4570625, rs11178997, rs120074175.Questionnaire survey was conducted on the case group and the control group.Chi-square test was used to compare the differences in the frequency distribution of alleles and genotype between two groups. The generalized multifactor dimensionality reduction ( GMDR) method was used to analyze the interaction between gene and environment.Binary logistic regression was used to verify the optimal model.Results After adjusting the factors of sex and age,the GMDR analysis showed rs4570625,rs11178997,rs120074175 and negative life events were the optimal model.In this model, the testing balanced accuracy was 0.7838 and cross-validation consistency value was 10/10.There was statistically significant effect on the risk of major de-pressive disorder ( P ＝ 0.001 ). Binary logistic regression analysis showed that individuals, who had rs11178997 A+ genotype (AA,AT),rs120074175 A+genotype (AA,AG) and negative life events,had sig-nificant OR values of 24.307(95%CI＝13.007-45.427) and 38.2502(95%CI＝1.148-69.181),showing a higher risk of depression.Conclusion The interaction between TPH2 gene (rs11178997,rs120074175) and negative life events plays an important role in depression.
