Rapamycin protect puromycin amino nucleoside induced podocyte injury by up-regulating autophagy
10.3969/j.issn.1000-484X.2018.02.006
- VernacularTitle:雷帕霉素激活自噬改善嘌呤霉素氨基核苷诱导的足细胞损伤
- Author:
Hui-Fang ZHAN
1
;
Wen-Juan LIU
;
Juan JIN
;
Jian-Guang GONG
;
Li ZHAO
;
Qiang HE
Author Information
1. 浙江大学校医院紫金港校区
- Keywords:
Rapamycin;
Puromycin amino nucleoside;
Podocyte;
Autophagy;
mTOR signaling pathway
- From:
Chinese Journal of Immunology
2018;34(2):188-191,198
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effect of rapamycin and the role of autophagy on podocyte injury induced by pu-romycin amino nucleoside.Methods:The mice podocytes cell 5(MPC5)were randomly divided into four groups:Control group,PAN group(50 μg/ml PAN);rapamycin group(RAP group,100 ng/ml,200 ng/ml,300 ng/ml rapamycin)and PAN+rapamycin group (PAN+RAP group,treated with rapamycin before PAN).The apoptosis of podocytes were detected by Annexin V/PI.Transmission electronic microscopy was used to observe the formation of autophagosome.The expression of autophagic markers,LC3,p62,and 4EBP1,P70S6K,mTOR were detected by Western blot.Results: The podocyte apoptotic rate was significantly decreased when the podocytes were treated with rapamycin compared with PAN group.Further,more autophagosomes appeared in cytoplasm of podocytes in RAP and PAN+RAP group compared with PAN group.And the expression of LC3Ⅱ was significantly increased in rapamycin treated podocytes,whereas the expression of p62 was decreased markedly.Phosphorylation of mTOR,4EBP1 and P70S6K was decreased rapamycin treated groups.Conclusion: Podocyte autophagy was inhibited by PAN, and the apoptosis of podocytes was promoted.Rapamycin protect puromycin amino nucleoside induced podocyte injury by upregulating autophagy and it may be concerned with mTOR 4EBP1 and P70S6K signaling pathway.
